Difference between revisions of "Team:TCU Taiwan/Modeling"

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<tr><td align="center"><h1><span style="font-family:Arial Black;"><font size="6"><font-weight: 700;>Macro Dilution</font></span></h1></td></tr>
 
<tr><td align="center"><h1><span style="font-family:Arial Black;"><font size="6"><font-weight: 700;>Macro Dilution</font></span></h1></td></tr>
 
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We had tested the disinfection ability of our AMPs and proved our system is really worked. We performed macro-dilution experiments. First, we prepared 1 ml LB medium of <I>S. aureus</I> concentration of OD 0.01 at 600 nm. Than we added 1 ml sample into tube and measured the OD at 600 nm once in two hours. We had analyzed the data and made it into chart.
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We had tested the disinfection ability of our AMPs and proved our system is really worked.First, we prepared 1 ml LB medium of <I>S. aureus</I> concentration of OD 0.01 at 600 nm. Than we added 1 ml sample into tube and measured the OD at 600 nm once in two hours. We had analyzed the data and made it into chart.
 
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     <td width="90%" align="center"><img src="https://static.igem.org/mediawiki/2015/a/a7/2015tcutaiwanThe_peptides_efficiency_of_disinfection.JPG" align=center width="60%"  title="Result 1"></td>
 
     <td width="90%" align="center"><img src="https://static.igem.org/mediawiki/2015/a/a7/2015tcutaiwanThe_peptides_efficiency_of_disinfection.JPG" align=center width="60%"  title="Result 1"></td>
 
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Conclusions: As the chart we can see that both Signiferin and Epinecidin-1 have ability to kill <I>S. aureus</I>. But the effect is not obvious we speculated the concentration is not enough to see the best efficiency. We will concentrate the peptides solution to do further experiments.
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Conclusions: As the chart we can see that both Signiferin and Epinecidin-1 have ability to kill <I>S. aureus</I>. But the effect is not obvious. We speculated the concentration is not enough to see the best efficiency. We will concentrate the peptides solution to do further experiments.
 
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Revision as of 21:50, 18 September 2015

About our modeling

Macro Dilution

We had tested the disinfection ability of our AMPs and proved our system is really worked.First, we prepared 1 ml LB medium of S. aureus concentration of OD 0.01 at 600 nm. Than we added 1 ml sample into tube and measured the OD at 600 nm once in two hours. We had analyzed the data and made it into chart.

Conclusions: As the chart we can see that both Signiferin and Epinecidin-1 have ability to kill S. aureus. But the effect is not obvious. We speculated the concentration is not enough to see the best efficiency. We will concentrate the peptides solution to do further experiments.

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