Day 1 Wet lab 22/06/15

Made LB plates

Made LB broth

TIPS autoclaved

Day 2 Wet lab 23/06/15

Set up Top10 overnight, VS45 overnight, US348 overnight

Meeting with advisors to discuss progress

Day 3 Wet lab 24/06/15

Filter sterilise the buffer

250ml no antibiotic broth, put top10 cells in

Incubate until optical density is 0.6

Miniprep PSB1C3 plasmid

Day 4 Wet lab 25/06/15

Transformation

Prepare SBC media

Digest of PSB1c3 from MS348

Day 5 Wet lab 26/06/15

Calculated competent cell efficiency

Agarose gel formation

Gel electrophoresis

Day 6 Wet lab 29/06/15

Transforming linear PSB1C3

Day 7 Wet lab 30/06/15

Miniprep of PSB1A3 plasmid

Gel electrophoresis of a large quantity of PSB1C3

Meeting with advisors to discuss progress

Day 8 Wet lab 01/07/15

Gel electrophoresis of the purified DNA extracted

Transformation of PSB1A3 circular plasmid to VS45 cells (competent)

Digest of all PSB1c3 plasmids

Day 9 Wet lab 02/07/15

Competent cells transformation efficiency

Mini-prep of 1MS340 to get PSB1c3 plasmid

Gel extraction of big kb digest

Quantification of the digest. Added sample buffer to each digest

Day 10 Wet lab 03/07/15

Transformation efficiency