Difference between revisions of "Team:Birkbeck/Results"

 
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<h1>Under Construction</h1>
 
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<p><b><u>Fig. 1: Growth Curve of <i>E. coli</i> DH5α Strains Following Culture Optical Density of 600 nm</u></b>.</p>
 
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<p><b><u>Fig. 2: Growth Curve of <i>E. coli</i> DH5α Strains Following Culture Optical Density of 395 nm</u></b>.</p>
 
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<center><img src="https://static.igem.org/mediawiki/2015/d/d7/Viable_count_1_hour_%28dh5_alpha%29_team_birkbeck_iGEM_2015.jpg"></center>
 
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<p><b><u>Fig. 3: Viable Count of <i>E. coli</i> DH5α After 60 mins</u></b>.</p>
 
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<p><b><u>Fig. 4: Viable Count of <i>E. coli</i> DH5α After 175 mins</u></b>.</p>
 
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<center><IMG SRC="https://static.igem.org/mediawiki/2015/8/8a/Growth_curve_601_nm_96-microtitre_Team_birkbeck_iGEM_2015_data.jpg "></center>
 
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<p><b><u>Fig. 5: Growth Curves of Different Strains of <i>E. coli</i> DH5α Following Culture Optical Density at 601 nm</u></b>.</p>
 
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<p><b><u>Fig. 6: Growth Curves of Different Strains of <i>E. coli</i> DH5α Following Culture Optical Density at 501 nm</u></b>.</p>
 
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<center><img src="https://static.igem.org/mediawiki/2015/4/4a/Growth_curve_475_nm_microtitre_team_birkbeck_iGEM_2015.jpg"></center>
 
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<p><b><u>Fig. 7: Growth Curves of Different Strains of <i>E. coli</i> DH5α Following Culture Optical Density at 475 nm</u></b>.</p>
 
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<center><img src="https://static.igem.org/mediawiki/2015/8/8b/Growth_curve_395_microtitre_team_birkbeck_iGEM_2015.jpg "></center>
 
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<p><b><u>Fig. 8: Growth Curves of Different Strains of <i>E. coli</i> DH5α Following Culture Optical Density at 395 nm</u></b>.</p>
 
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<center><img src="https://static.igem.org/mediawiki/2015/9/9a/Fluorescence_growth_curve_of_multiple_strains_of_E._coli_DH5_alpha_team_Birkbeck_iGEM_2015.jpg "></center>
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<p><b><u>Fig. 9: Growth Curves of Different Strains of <i>E. coli</i> DH5α Following Culture Fluorescence</u></b>.</p>
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<h2> Project Results</h2>
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<li> Clearly and objectively describe the results of your work.</li>
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<li> Future plans for the project </li>
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<h2><b>Results</b></h2>
  
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<h4> Project Achievements </h4>
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                <a href="#">BioBrick Cloning Results
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                    <p>Click here to see the results from testing the constructs ORF-314, stf gene, tfa construct, P(Cat)-TetR construct, TetR-controlled tfa circuit and cI-Cro circuit.
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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    REPLACE WITH PICTURE TO REPRESENT THIS SECTION
  
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<p>To confirm the presence and correct size of the BioBricks we are submitting, all samples were run on an agarose gel. The constructs tested were:
 
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<li>A list of linked bullet points of the successful results during your project</li>
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<li>ORF-314</li>
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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<li>the stf gene</li>  
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<li>the tfa construct</li>  
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<li>the P(Cat)-TetR construct</li>
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<li>the TetR-controlled tfa circuit</li>
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<li>the cI-Cro circuit</li></ul></p>
  
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<p>All samples were initially digested with two of the four standard iGEM restriction enzymes (<i>XbaI</i>, <i>EcoRI</i>, <i>SpeI</i> and <i>PstI</i>) and expected fragment sizes calculated (Fig 1a and 2a). Success of the cloning was then determined by comparing actual band sizes to the predicted values (Fig 1b and 2b).
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<p>a.&nbsp;<IMG SRC="https://static.igem.org/mediawiki/2015/e/e8/Birkbeck_150916_LP_gel1_pred.png" height="600px" width="300px">
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<p><b>Fig 1.</b> Comparison of predicted (a) and actual (b) band sizes on gel 1.</p>
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<p>a.&nbsp;<IMG SRC="https://static.igem.org/mediawiki/2015/6/60/Birkbeck_150916_LP_gel2_pred.png" height="600px" width="300px">
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<p><b>Fig 2.</b> Comparison of predicted (a) and actual (b) band sizes on gel 2.</p>
  
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<h4>Inspiration</h4>
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<p>See how other teams presented their results.</p>
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            <br><hr><br>
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
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<a href="https://2015.igem.org/Team:Birkbeck/Conclusion"><img width="400px" height="auto" src="https://static.igem.org/mediawiki/2015/7/7d/Birkbeck_phage_infection2.png" border="0"/></a>
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</ul>
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<p>To find concluding remarks about our project</p>
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<!--button for Research-->
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<a href="https://2015.igem.org/Team:Birkbeck/Research"><img width="400px" height="auto" src="https://static.igem.org/mediawiki/2015/c/c7/Rachel-elliot.jpg" border="0"/></a>
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<p>Back to main Research page</p>
  
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Latest revision as of 22:42, 18 September 2015

jQuery UI Accordion - Collapse content

Results

BioBrick Cloning Results

Click here to see the results from testing the constructs ORF-314, stf gene, tfa construct, P(Cat)-TetR construct, TetR-controlled tfa circuit and cI-Cro circuit.


To confirm the presence and correct size of the BioBricks we are submitting, all samples were run on an agarose gel. The constructs tested were:

  • ORF-314
  • the stf gene
  • the tfa construct
  • the P(Cat)-TetR construct
  • the TetR-controlled tfa circuit
  • the cI-Cro circuit

All samples were initially digested with two of the four standard iGEM restriction enzymes (XbaI, EcoRI, SpeI and PstI) and expected fragment sizes calculated (Fig 1a and 2a). Success of the cloning was then determined by comparing actual band sizes to the predicted values (Fig 1b and 2b).


a.  b. 

Fig 1. Comparison of predicted (a) and actual (b) band sizes on gel 1.


a.  b. 

Fig 2. Comparison of predicted (a) and actual (b) band sizes on gel 2.




To find concluding remarks about our project

Back to main Research page