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| − | <h1>Under Construction</h1>
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| − | <!--<IMG SRC="https://static.igem.org/mediawiki/2015/e/ea/Viable_count_E.coli_DH5alpha_checking_quality_team_Birkbeck_iGEM_2015.jpg">-->
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| − | <!--<p>The growth kinetics of E. coli DH5α had to be established if experiments involving infection with λ-bacteriophage and characterizing a potential signal in living cells. It was important for the study to also quantify the number of viable cells with relation to the optical density of the culture.</p>
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| − | <center><IMG SRC="https://static.igem.org/mediawiki/2015/5/58/Growth_Curve_50_mL_600_nm_team_birkbeck_iGEM_2015.jpg"></center>
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| − | <p><b><u>Fig. 1: Growth Curve of <i>E. coli</i> DH5α Strains Following Culture Optical Density of 600 nm</u></b>.</p>
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| − | <br>
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| − | <!--<p>Growth kinetics was initially studied using 50 mL cultures. Fig. 1 shows the growth kinetics of E. coli DH5α & derivative strains containing plasmids from the <a href="https://2015.igem.org/Team:Birkbeck/InterLab_Study">InterLab</a> study. The growth curve shows that the <i>E. coli</i> strain that contains the <i>gfp</i> expression device P1 grows at a slower rate than the other strains investigated. At 220 minutes the <i>E. coli</i> DH5α P1 strain has a significantly lower OD<sup>600</sub> than the <i>E. coli</i> DH5α (P=0.023). <i>E. coli</i> DH5α remains very highly significantly higher in OD600 than E. coli DH5α with the P1-gfp expression device (P=<0.001). The only difference between the E. coli DH5α & E. coli DH5α positive control device is observed at 280 minutes into the growth curve (P=0.016) where the positive control has a higher OD<sub>600</sub>. The multiple comparison table showing P values can be viewed in <a href="https://2015.igem.org/Team:Birkbeck/Results/Table_S1"></a><b>Table S1</b></p>-->
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| − | <center><img src="https://static.igem.org/mediawiki/2015/a/a8/Growth_Curve_%28395_nm%29_50_mL_Cultures_Team_birkbeck_iGEM_2015.jpg "></center>
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| − | <p><b><u>Fig. 2: Growth Curve of <i>E. coli</i> DH5α Strains Following Culture Optical Density of 395 nm</u></b>.</p>
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| − | <br>
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| − | <!--<p>In order to investigate if there could be a point in the <i>E. coli</i> DH5α growth curve in which a signal from the GFP could be detected by absorbance, the growth curves were also conducted using the major absorption peak of GFP (wavelength 395 nm) (REF!). The growth curve data for the culture optical density is displayed in Fig. 2.</P>-->
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| − | <!--Multiple Comparison table link for OD 395 nm (table S2); https://2015.igem.org/Team:Birkbeck/Results/Table_S2. issue sorted, this page is good to go!.--> | + | <head> |
| − | <!--For sorting out code to make it look decent on our wiki; https://2015.igem.org/Team:Birkbeck/Results/adjusting_table_code ; use this link. its a spoof web page.-->
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| − | <center><img src="https://static.igem.org/mediawiki/2015/d/d7/Viable_count_1_hour_%28dh5_alpha%29_team_birkbeck_iGEM_2015.jpg"></center>
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| − | <p><b><u>Fig. 3: Viable Count of <i>E. coli</i> DH5α After 60 mins</u></b>.</p>
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| − | <!--Start of descriptive stats table-->
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| − | <table class="Default PivotTable" data-lookName="Default" data-rowLabelWidth="1" data-colLabelHeight="1" data-NumRows="2" data-NumCols="5" data-rowLabels="Corner" data-decimal-char="." data-col-labels="N;Minimum;Maximum;Mean;Std. Deviation" data-row-labels="Viable.Count;Valid N (listwise)" >
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| − | <caption class="title" style="width:522px">Descriptive Statistics of 1 hour Viable Count.<span class="details"></span></caption>
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| − | <td class="columnLabels dataAreaLeft vCC role3">N</td>
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| − | <td class="columnLabels vCC role3">Minimum</td>
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| − | <td class="columnLabels vCC role3">Maximum</td>
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| − | <td class="columnLabels vCC role3">Mean</td>
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| − | <td class="columnLabels vCC role3">Std. Deviation</td>
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| − | </tr>
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| − | <td class="rowLabels dataAreaTop role3">Viable.Count</td>
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| − | <td class=" e dataAreaTop dataAreaLeft vCC">9</td>
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| − | <td class=" e dataAreaTop vCC">1600000</td>
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| − | <td class=" e dataAreaTop vCC">3750000</td>
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| − | <td class=" e dataAreaTop vCC">2566666.67</td>
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| − | <td class=" e dataAreaTop vCC">627495.020</td>
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| − | </tr>
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| − | <tr>
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| − | <td class="rowLabels hCR role3">Valid N (listwise)</td>
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| − | <td class=" o dataAreaLeft hCR vCC">9</td>
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| − | <td class=" o hCR vCC"> </td>
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| − | <td class=" o hCR vCC"> </td>
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| − | <td class=" o hCR vCC"> </td>
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| − | </tr>
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| − | </tbody>
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| − | </table>
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| − | </div></body>
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| − | <!--End of descriptive Stats table-->
| + | |
| − | <p><b><u>Table 1: Descriptive Statistics of 60 minutes Viable Count</u></b>.</p>
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| − | <center><img src="https://static.igem.org/mediawiki/2015/e/e6/Viable_count_of_DH5_alpha_after_175_mins_team_birkbeck_iGEM_2015.jpg"></center>
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| − | <p><b><u>Fig. 4: Viable Count of <i>E. coli</i> DH5α After 175 mins</u></b>.</p>
| + | |
| − | <br>
| + | |
| − | <!--Begining of the descriptive statistics table for 175 minutes of growth-->
| + | |
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| − | <!--End of the descriptive statistics table for 175 minutes of growth--> | + | <div class="accordion"> |
| − | <center><IMG SRC="https://static.igem.org/mediawiki/2015/8/8a/Growth_curve_601_nm_96-microtitre_Team_birkbeck_iGEM_2015_data.jpg "></center>
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| − | <p><b><u>Fig. 5: Growth Curves of Different Strains of <i>E. coli</i> DH5α Following Culture Optical Density at 601 nm</u></b>.</p>
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| − | <br>
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| − | <center><IMG SRC="https://static.igem.org/mediawiki/2015/d/d0/Growth_curve_501_nm_microtitre_data_Team_birkbeck_iGEM_2015.jpg"></center>
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| − | <p><b><u>Fig. 6: Growth Curves of Different Strains of <i>E. coli</i> DH5α Following Culture Optical Density at 501 nm</u></b>.</p>
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| − | <br>
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| − | <center><img src="https://static.igem.org/mediawiki/2015/4/4a/Growth_curve_475_nm_microtitre_team_birkbeck_iGEM_2015.jpg"></center>
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| − | <p><b><u>Fig. 7: Growth Curves of Different Strains of <i>E. coli</i> DH5α Following Culture Optical Density at 475 nm</u></b>.</p>
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| − | <br>
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| − | <center><img src="https://static.igem.org/mediawiki/2015/8/8b/Growth_curve_395_microtitre_team_birkbeck_iGEM_2015.jpg "></center>
| + | |
| − | <p><b><u>Fig. 8: Growth Curves of Different Strains of <i>E. coli</i> DH5α Following Culture Optical Density at 395 nm</u></b>.</p>
| + | |
| − | <br>
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| − | <center><img src="https://static.igem.org/mediawiki/2015/9/9a/Fluorescence_growth_curve_of_multiple_strains_of_E._coli_DH5_alpha_team_Birkbeck_iGEM_2015.jpg "></center> | + | <center> |
| − | <p><b><u>Fig. 9: Growth Curves of Different Strains of <i>E. coli</i> DH5α Following Culture Fluorescence</u></b>.</p> | + | <h3> |
| − | <br> | + | <a href="#">BioBrick Cloning Results |
| − | <!-- | + | </h3> |
| − | <h2> Project Results</h2>
| + | <p> Click here to see the results from testing the constructs ORF-314, stf gene, tfa construct, P(Cat)-TetR construct, TetR-controlled tfa circuit and cI-Cro circuit. |
| | + | </p> |
| | + | <br> |
| | + | <!-- ############################################################################# |
| | | | |
| − | <p>Here you can describe the results of your project and your future plans. </p>
| + | REPLACE WITH PICTURE TO REPRESENT THIS SECTION |
| | | | |
| − | <h5>What should this page contain?</h5>
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| − | <ul>
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| − | <li> Clearly and objectively describe the results of your work.</li>
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| − | <li> Future plans for the project </li>
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| − | <li> Considerations for replicating the experiments </li>
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| − | </ul>
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| | + | <img width="800" height="auto" src="https://static.igem.org/mediawiki/2015/f/f9/20150914_122201.jpg" border="0"/> |
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| | | | |
| | + | #############################################################################--> |
| | + | </a> |
| | + | </h3> |
| | + | |
| | + | </center> |
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| | + | <div> |
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| − | <h4> Project Achievements </h4>
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| − |
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| − | <p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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| | + | <div style="font-size: 16px;"> |
| | + | <p>To confirm the presence and correct size of the BioBricks we are submitting, all samples were run on an agarose gel. The constructs tested were: |
| | <ul> | | <ul> |
| − | <li>A list of linked bullet points of the successful results during your project</li> | + | <li>ORF-314</li> |
| − | <li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li> | + | <li>the stf gene</li> |
| − | </ul> | + | <li>the tfa construct</li> |
| | + | <li>the P(Cat)-TetR construct</li> |
| | + | <li>the TetR-controlled tfa circuit</li> |
| | + | <li>the cI-Cro circuit</li></ul></p> |
| | | | |
| | + | <p>All samples were initially digested with two of the four standard iGEM restriction enzymes (<i>XbaI</i>, <i>EcoRI</i>, <i>SpeI</i> and <i>PstI</i>) and expected fragment sizes calculated (Fig 1a and 2a). Success of the cloning was then determined by comparing actual band sizes to the predicted values (Fig 1b and 2b). |
| | + | </div> |
| | + | <br> |
| | | | |
| | + | <p>a. <IMG SRC="https://static.igem.org/mediawiki/2015/e/e8/Birkbeck_150916_LP_gel1_pred.png" height="600px" width="300px"> |
| | + | b. <IMG SRC="https://static.igem.org/mediawiki/2015/1/18/Birkbeck_150916_LP_gel1.jpeg" height="600px" width="350px"></p> |
| | + | <p><b>Fig 1.</b> Comparison of predicted (a) and actual (b) band sizes on gel 1.</p> |
| | + | <br> |
| | + | <p>a. <IMG SRC="https://static.igem.org/mediawiki/2015/6/60/Birkbeck_150916_LP_gel2_pred.png" height="600px" width="300px"> |
| | + | b. <IMG SRC="https://static.igem.org/mediawiki/2015/f/f9/Birkbeck_150916_LP_gel2.jpeg" height="600px" width="350px"></p> |
| | + | <p><b>Fig 2.</b> Comparison of predicted (a) and actual (b) band sizes on gel 2.</p> |
| | | | |
| − | <h4>Inspiration</h4>
| + | </div> |
| − | <p>See how other teams presented their results.</p> | + | <br><hr><br> |
| − | <ul> | + | </div> |
| − | < li><a href="https:// 2014.igem.org/Team: TU_Darmstadt/ Results/Pathway"> 2014 TU Darmstadt < /a></li> | + | <br> |
| − | <li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
| + | <!--button for Conclusion--> |
| − | < li><a href="https:// 2014.igem.org/Team: Paris_Bettencourt/ Results"> 2014 Paris Bettencourt </ a></ li> | + | <a href="https://2015.igem.org/Team:Birkbeck/Conclusion"><img width="400px" height="auto" src="https://static.igem.org/mediawiki/2015/7/7d/Birkbeck_phage_infection2.png" border="0"/></a> |
| − | </ul> | + | <p>To find concluding remarks about our project</p> |
| | + | <!--button for Research--> |
| | + | <a href="https://2015.igem.org/Team:Birkbeck/Research"><img width="400px" height="auto" src="https://static.igem.org/mediawiki/2015/c/c7/Rachel-elliot.jpg" border="0"/></a> |
| | + | <p>Back to main Research page</p> |
| | | | |
| | + | </div> |
| | </div> | | </div> |
| | </html> | | </html> |
b. 
b. 
