Difference between revisions of "Team:Aachen/Lab/Methanol/Biobricks"
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− | Subsequently, we designed and ordered linear gBlocks with additional extensions up- and downstream the coding sequence. Each of the 58 bp upstream extensions contain an overlap to [http://parts.igem.org/Part: | + | Subsequently, we designed and ordered linear gBlocks with additional extensions up- and downstream the coding sequence. Each of the 58 bp upstream extensions contain an overlap to [http://parts.igem.org/Part:pSB1C3 pSB1C3], the BioBrick prefix and the [http://parts.igem.org/Part:BBa_B0034 BBa_B0034] ribosome binding site plus the BioBrick scar TAGTAC. Downstream the CDS, we added an 41 bp extension with the suffix and another overlapping region to [http://parts.igem.org/Part:pSB1C3 pSB1C3]. |
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! BioBrick !! construct !! sequencing confirmed plasmid !! confirmed cryo | ! BioBrick !! construct !! sequencing confirmed plasmid !! confirmed cryo | ||
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− | | [http://parts.igem.org/Part:BBa_K1585210 BBa_K1585210] || RBS. | + | | [http://parts.igem.org/Part:BBa_K1585210 BBa_K1585210] || RBS.mdh in [http://parts.igem.org/Part:pSB1C3 pSB1C3] || #9PBD# || #WNFY# |
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− | | [http://parts.igem.org/Part:BBa_K1585211 BBa_K1585211] || RBS. | + | | [http://parts.igem.org/Part:BBa_K1585211 BBa_K1585211] || RBS.hps in [http://parts.igem.org/Part:pSB1C3 pSB1C3] || #H4M9# || #863A# |
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− | | [http://parts.igem.org/Part:BBa_K1585212 BBa_K1585212] || RBS. | + | | [http://parts.igem.org/Part:BBa_K1585212 BBa_K1585212] || RBS.phi in [http://parts.igem.org/Part:pSB1C3 pSB1C3] || #ENKK# || #AE8V# |
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− | | [http://parts.igem.org/Part:BBa_K1585213 BBa_K1585213] || RBS. | + | | [http://parts.igem.org/Part:BBa_K1585213 BBa_K1585213] || RBS.xpk in [http://parts.igem.org/Part:pSB1C3 pSB1C3] || #KFEY# || #8WY9# |
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Latest revision as of 23:29, 18 September 2015