Difference between revisions of "Team:Chalmers-Gothenburg/Project"

 
(10 intermediate revisions by 3 users not shown)
Line 1: Line 1:
{{Template_All_Teams}}
+
{{Chalmers-Gothenburg}}
  
<!-- Declare that you are going to use html code instead of wiki code -->
+
<h2> Project </h2>
<html>
+
 
+
<!-- Start of CSS-->
+
<style type="text/css">
+
 
+
/* PAGE LAYOUT */
+
 
+
/* Change Background color*/
+
body {
+
background-color: #fff;
+
}
+
 
+
/* Creates a container that will wrap all of the content inside your wiki pages. */
+
#mainContainer { 
+
width: 978px;
+
overflow:hidden;
+
float:left;
+
margin-left:20px;
+
margin-bottom: 10px;
+
background-color: #fff;
+
border-bottom: 14px solid #565656;
+
border-right: 2px solid #565656;
+
border-left: 2px solid #565656;
+
border-top: 2px solid #565656;
+
font-family: "Trebuchet MS", Helvetica, sans-serif;
+
}
+
 
+
/* Creates the container for the menu */
+
#menuContainer  {
+
float:left;
+
width: 134px;
+
padding: 20px 0px;
+
border-top: 14px solid #565656;
+
background-color: #E8E8E9;
+
}
+
 
+
/* Creates the container for the content */
+
#contentContainer {
+
padding-top:20px;
+
padding-right:10px;
+
margin-bottom: 20px;
+
width: 814px;
+
padding-left: 20px;
+
float: left;
+
background-color: #fff;
+
border-top: 14px solid #565656;
+
font-family: "Trebuchet MS", Helvetica, sans-serif;
+
}
+
 
+
/*Set up height place holder for the banner*/
+
#bannerContainer {
+
height:200px;
+
margin:auto;
+
text-align:center;
+
color: #24B694;
+
}
+
 
+
/*VISUAL STYLE (TABLES, FONTS, LINKS, IMAGES, UL) */
+
 
+
/*Change the styling of text for everything inside main container*/
+
#mainContainer p {
+
font-size: 13px;
+
color: #000000;
+
}
+
+
/*This changes the color and font family */
+
#contentContainer h1, h2, h3, h4, h5, h6 {
+
color: #565656;
+
border-bottom: none;
+
font-weight: bold;
+
font-family: "Trebuchet MS", Helvetica, sans-serif;
+
margin-top:10px;
+
}
+
 
+
/*Style of the links - links are different inside the menu */
+
#contentContainer a {
+
font-weight: bold;
+
color: #23b593;
+
}
+
 
+
  /* Styling links on hover- links are different inside the menu */
+
#contentContainer a:hover {
+
color: #59bf92;
+
}
+
 
+
/*Change the styling of tables */
+
        #contentContainer table {
+
    border: 1px solid #565656;
+
    border-collapse: collapse;
+
    width: 90%
+
    margin: auto;
+
    margin-bottom: 15px;
+
    margin-top: 15px;
+
    margin-right: 10px;
+
    margin-left: 10px;
+
    }
+
 
+
/*Change the styling of table cells*/
+
    #contentContainer  td {
+
    padding: 10px;
+
    border: 1px solid #565656;
+
    border-collapse: collapse;
+
    vertical-align: text-top;
+
    }
+
 
+
/*Change the styling of table headers */
+
    #contentContainer th {
+
    background-color: #E8E8E9;
+
    padding: 10px;
+
    border: 1px solid #565656;
+
    border-collapse: collapse;
+
    vertical-align: text-top;
+
    }
+
 
+
 
+
 
+
/*MENU STYLING */
+
 
+
/*Styling for the links in the menu */
+
#menuContainer a {
+
color: #565656;
+
text-decoration:none;
+
font-weight: bold;
+
}
+
 
+
/* Sets the style for lists inside menuContainer  */
+
#menuContainer ul {
+
list-style: none;
+
margin-left:0px;
+
}
+
 
+
/*Styles the list items to become menu buttons */
+
#menuContainer ul li {
+
text-align: center;
+
display: block;
+
width: 100%;
+
height:30px;
+
padding-top:10px;
+
}
+
 
+
/*For the menu buttons, changes the color when hovering*/
+
#menuContainer li:hover {
+
color:  #FFF;
+
background-color: #24B694;
+
}
+
 
+
/*Submenus are not displayed as default*/
+
#menuContainer li ul {
+
display: none;
+
padding-top:15px;
+
margin-left: -19px;
+
}
+
 
+
/*Submenus are displayed when hovering the menu button */
+
#menuContainer li:hover ul {
+
/*display: inline-block; */
+
display: block;
+
position: absolute;
+
float:right;
+
margin-left: 134px;
+
margin-top:-42px;
+
}
+
 
+
/*Style the submenu buttons*/
+
#menuContainer li ul li{
+
background-color: #59BF92;
+
padding-left:20px;
+
padding-right:20px;
+
height:30px;
+
padding-top:10px;
+
margin-top:-2px;
+
color: #565656;
+
width: 150px;
+
}
+
 
+
/*CLASSES */
+
 
+
/*Clear class for all the pages, adds spacing too*/
+
.clear{
+
clear:both;
+
height: 10px;
+
}
+
 
+
 
+
/* highlight box for special messages */
+
    .highlightBox {
+
        width:500px;
+
        margin:auto;
+
        background-color: #E8E8E9;
+
margin-bottom: 15px;
+
margin-top: 15px;
+
padding: 15px;
+
padding-top: 5px;
+
    }
+
 
+
 
+
</style>
+
<!-- End of CSS -->
+
 
+
 
+
<!-- Start of the template html elements. -->
+
<div id ="mainContainer"><!--The closing tag for mainContainer should be placed at the bottom of each content page.-->
+
 
+
 
+
<div id="bannerContainer">
+
<br><br>
+
<h2> Add a banner to your wiki! </h2>
+
+
<p>You can make the image 980px  by  200px</p>
+
<p> Remember to call the file: "<i>Team_Chalmers-Gothenburg_banner.jpg</i>" </p>
+
</div>
+
 
+
<!-- Start of menu -->
+
<div id="menuContainer">
+
 
+
<!-- This list is your menu, every list item is a menu button and nested listed become submenu buttons -->
+
<ul>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg"><li>HOME</li></a>
+
 
+
 
+
 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Team"><li>TEAM
+
<ul> 
+
 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Students"><li>Students</li></a>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Supervisors"><li>Supervisors</li></a> 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Attributions"><li>Attributions</li></a>   
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Gallery"><li>Gallery</li></a>
+
+
</ul>
+
 
+
</li></a>
+
 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Project"><li>PROJECT
+
<ul> 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Theory"><li>Theory</li></a> 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Constructs"><li>Constructs</li></a>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Protocols"><li>Protocols</li></a>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/OtherApplications"><li>Other applications</li></a>
+
</ul>
+
</li></a>
+
 
+
 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Achievements"><li>ACHIEVEMENTS
+
<ul> 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Project Results"><li>Project Results</li></a>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/BioBricks"><li>BioBricks</li></a> 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Medals"><li>Medals</li></a> 
+
+
</ul>
+
</li></a>
+
 
+
 
+
<a href="#"><li>MODELING
+
            <ul>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Detection"><li>Detection</li></a>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Safety Switch"><li>Safety Switch</li></a> 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Simulation"><li>Simulation</li></a> 
+
+
</ul>
+
</li></a>
+
+
 
+
 
+
 
+
+
 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Practices"><li>HUMAN PRACTICES
+
<ul>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/iGEMCommunity"><li>iGEM Community</li></a>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/NextGeneration"><li>Next Generation</li></a> 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Society"><li>Society</li></a> 
+
+
</ul></a></li>
+
 
+
 
+
 
+
 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Notebook"><li>NOTEBOOK
+
<ul>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Schedule"><li>Schedule</li></a>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Attributions"><li>Attributions</li></a>   
+
+
</ul>
+
</li></a>
+
     
+
+
 
+
 
+
 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Safety"><li>SAFETY
+
<ul>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/LabSafety"><li>Lab Safety</li></a>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Ethics"><li>Ethics</li></a> 
+
+
</ul>
+
 
+
</li></a>
+
 
+
+
 
+
 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Entrepreneurship"><li>THANKS
+
 
+
<ul>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Sponsors"><li>Sponsors</li></a>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Acknowledgements"><li>Acknowledgements</li></a> 
+
+
</ul>
+
 
+
</li></a>
+
 
+
</ul>
+
</div>
+
<!-- End of menu  -->
+
 
+
<!-- Start of content -->
+
<div id="contentContainer"> <!--The closing tag for contentContainer should be placed at the bottom of each content page.-->
+
</html>
+
 
+
 
+
<h1> Project </h1>
+
  
 
<p>Contaminations – a problem you definitely want to get rid of as quickly as possible. It can destroy your product and lower the efficiency of the process, which in the end may lead to higher expenses. We have created a great system for both finding and combating contaminations in bioreactors, automatically! Our intention was to deal with the hard process in finding unwanted cells in bioreactors and our solution is an innovative system that is both time and material efficient. With our project you no longer need to worry about long analytic times and if your product is pure enough, it will all be taken care of directly in the process! Do you know what the best part is? The system is really cool and can be used for so much more!</p>
 
<p>Contaminations – a problem you definitely want to get rid of as quickly as possible. It can destroy your product and lower the efficiency of the process, which in the end may lead to higher expenses. We have created a great system for both finding and combating contaminations in bioreactors, automatically! Our intention was to deal with the hard process in finding unwanted cells in bioreactors and our solution is an innovative system that is both time and material efficient. With our project you no longer need to worry about long analytic times and if your product is pure enough, it will all be taken care of directly in the process! Do you know what the best part is? The system is really cool and can be used for so much more!</p>
  
<p>Our project presents a solution for dealing with contamination in bioreactors. We have found a way to detect and deal with contamination without wasting product in the tank. The solution could be used both for academic and industrial use. We have designed a two system solution that we have implemented in Saccharomyces cerevisiae, a solution that is both efficient and timesaving. The systems are based on one detection system that gives a fluorescent signal when a contaminant is present and one DNA-repair-system. The detection system uses pheromone detection to sense the present of a contaminant and uses an intracellular cascade reaction to send out a RFP-signal. The repair system is based on a system from the bacterium Deinococcus radiodurans and repairs blunt DNA-cuts, which are hard for the cell to repair. This system makes it possible to irradiate the tank with UV-irradiation that will kill the contaminant but not the yeast. These systems together with a safety switch for use in research will be presented in detail in this chapter.</p>
+
<p>Our project presents a solution for dealing with contamination in bioreactors. We have found a way to detect and deal with contamination without wasting product in the tank. The solution could be used both for academic and industrial use. We have designed a two system solution that we have implemented in <i>Saccharomyces cerevisiae</i>, a solution that is both efficient and time saving. The systems are based on one detection system that gives a fluorescent signal when a contaminant is present and one DNA-repair-system. The detection system uses pheromone detection to sense the present of a contaminant and uses an intracellular cascade reaction to send out a RFP-signal. The repair system is based on a system from the bacterium <i>Deinococcus radiodurans</i> and repairs blunt DNA-cuts, which are hard for the cell to repair. This system makes it possible to irradiate the tank with UV-irradiation that will kill the contaminant but not the yeast. These systems together with a safety switch for use in research will be presented in detail in this chapter.</p> <p>Figure 1-6 illustrates the general concept of our project.</p>  
  
 
[[File:ChalmersGothenburgPurTHEORY1.png|400px|]]
 
[[File:ChalmersGothenburgPurTHEORY1.png|400px|]]
 
+
<p class="figuretext"><b>Figure 1.</b> The bioreactor in its initial state containing only the producing organism(yellow), <i>Saccharomyces cerevisiae</i>.</p>
  
 
[[File:ChalmersGothenburgPurTHEORY2.png|400px|]]
 
[[File:ChalmersGothenburgPurTHEORY2.png|400px|]]
 +
<p class="figuretext"><b>Figure 2.</b> Contaminants(blue) getting into the reactor. </p>
  
 
[[File:ChalmersGothenburgPurTHEORY3.png|400px|]]
 
[[File:ChalmersGothenburgPurTHEORY3.png|400px|]]
 +
<p class="figuretext"><b>Figure 3.</b>Producing <i>S. cerevisiae</i> cells expressing RFP(red) upon detection, signaling the presence of contaminants. </p>
  
 
[[File:ChalmersGothenburgPurTHEORY4.png|400px|]]
 
[[File:ChalmersGothenburgPurTHEORY4.png|400px|]]
 +
<p class="figuretext"><b>Figure 4.</b> Detection signal initiating the irradiation process, its purpose being to combat the contaminants. </p>
  
 
[[File:ChalmersGothenburgPurTHEORY5.png|400px|]]
 
[[File:ChalmersGothenburgPurTHEORY5.png|400px|]]
 +
<p class="figuretext"><b>Figure 5.</b> Only modified <i>S. cerevisiae</i> cells survive the radiation due to the implemented repair system. Contaminants and modified cells that have expressed the detection signal are washed out. </p>
  
[[File:ChalmersGothenburgPurTHEORY5.png|400px|]]
+
[[File:ChalmersGothenburgPurTHEORY1.png|400px|]]
 +
<p class="figuretext"><b>Figure 6.</b> The bioreactor eventually returns to its original state.</p>
 +
 
 +
<html>
 +
</div>
 +
</div>
 +
</div>
 +
</div>

Latest revision as of 01:03, 19 September 2015

Project

Contaminations – a problem you definitely want to get rid of as quickly as possible. It can destroy your product and lower the efficiency of the process, which in the end may lead to higher expenses. We have created a great system for both finding and combating contaminations in bioreactors, automatically! Our intention was to deal with the hard process in finding unwanted cells in bioreactors and our solution is an innovative system that is both time and material efficient. With our project you no longer need to worry about long analytic times and if your product is pure enough, it will all be taken care of directly in the process! Do you know what the best part is? The system is really cool and can be used for so much more!

Our project presents a solution for dealing with contamination in bioreactors. We have found a way to detect and deal with contamination without wasting product in the tank. The solution could be used both for academic and industrial use. We have designed a two system solution that we have implemented in Saccharomyces cerevisiae, a solution that is both efficient and time saving. The systems are based on one detection system that gives a fluorescent signal when a contaminant is present and one DNA-repair-system. The detection system uses pheromone detection to sense the present of a contaminant and uses an intracellular cascade reaction to send out a RFP-signal. The repair system is based on a system from the bacterium Deinococcus radiodurans and repairs blunt DNA-cuts, which are hard for the cell to repair. This system makes it possible to irradiate the tank with UV-irradiation that will kill the contaminant but not the yeast. These systems together with a safety switch for use in research will be presented in detail in this chapter.

Figure 1-6 illustrates the general concept of our project.

ChalmersGothenburgPurTHEORY1.png

Figure 1. The bioreactor in its initial state containing only the producing organism(yellow), Saccharomyces cerevisiae.

ChalmersGothenburgPurTHEORY2.png

Figure 2. Contaminants(blue) getting into the reactor.

ChalmersGothenburgPurTHEORY3.png

Figure 3.Producing S. cerevisiae cells expressing RFP(red) upon detection, signaling the presence of contaminants.

ChalmersGothenburgPurTHEORY4.png

Figure 4. Detection signal initiating the irradiation process, its purpose being to combat the contaminants.

ChalmersGothenburgPurTHEORY5.png

Figure 5. Only modified S. cerevisiae cells survive the radiation due to the implemented repair system. Contaminants and modified cells that have expressed the detection signal are washed out.

ChalmersGothenburgPurTHEORY1.png

Figure 6. The bioreactor eventually returns to its original state.