Difference between revisions of "Team:William and Mary/Notebook"
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<p><span>Detail</span>4</p> | <p><span>Detail</span>4</p> | ||
<br> | <br> | ||
| − | <p> | + | <p>Week 1 (7/1-7/7) |
| + | Resuspended all parts from the kit | ||
| + | Designed Gibson assemblies to put various fluorescent proteins under the control of the same promoter, to get noise measurements | ||
| + | Began performing Gibson PCRs | ||
| + | Week 2 (7/8-7/14) | ||
| + | Troubleshot Gibson PCRs | ||
| + | Attempted first Gibson assembly | ||
| + | Designed Gibson assemblies to put together different fluorescent proteins, each under the control of the same promoter | ||
| + | Attempted to Gibson assemble YFP + CFP under control of R0010 | ||
| + | Week 3 (7/15-7/21) | ||
| + | Re-attempted Gibson assembly to make a double fluorescent construct with proteins under R0010 | ||
| + | Began assembling parts for the Interlab Study | ||
| + | Received gBlocks to assemble dCas9 and some preliminary gRNAs | ||
| + | Began assembling dCas9 and gRNAs | ||
| + | Began learning how to use confocal microscopy | ||
| + | Began assembling different promoters with our fluorescent constructs (to eventually collect noise data for each promoter) | ||
| + | Designed and started the assembly process to make KanR (necessary to integrate parts onto the E. coli genome) | ||
| + | Week 4 (7/22-7/28) | ||
| + | Many of our past assemblies (dCas9, double fluorescent constructs, etc) failed. We continued attempts at these assemblies. | ||
| + | </p> | ||
<p>Improved own provided blessing may peculiar domestic. Sight house has sex never. No visited raising gravity outward subject my cottage mr be. Hold do at tore in park feet near my case. Invitation at understood occasional sentiments insipidity inhabiting in. Off melancholy alteration principles old. Is do speedily kindness properly oh. Respect article painted cottage he is offices parlors. </p> | <p>Improved own provided blessing may peculiar domestic. Sight house has sex never. No visited raising gravity outward subject my cottage mr be. Hold do at tore in park feet near my case. Invitation at understood occasional sentiments insipidity inhabiting in. Off melancholy alteration principles old. Is do speedily kindness properly oh. Respect article painted cottage he is offices parlors. </p> | ||
</div> | </div> | ||
Revision as of 01:10, 19 September 2015
Our Notebook On this page you can view the work we did each month, from June through September. You can also find our protocols. Important Protocols Easy access to our most complex protocols. Detail1 Detail2 Detail3 Detail4 Week 1 (7/1-7/7)
Resuspended all parts from the kit
Designed Gibson assemblies to put various fluorescent proteins under the control of the same promoter, to get noise measurements
Began performing Gibson PCRs
Week 2 (7/8-7/14)
Troubleshot Gibson PCRs
Attempted first Gibson assembly
Designed Gibson assemblies to put together different fluorescent proteins, each under the control of the same promoter
Attempted to Gibson assemble YFP + CFP under control of R0010
Week 3 (7/15-7/21)
Re-attempted Gibson assembly to make a double fluorescent construct with proteins under R0010
Began assembling parts for the Interlab Study
Received gBlocks to assemble dCas9 and some preliminary gRNAs
Began assembling dCas9 and gRNAs
Began learning how to use confocal microscopy
Began assembling different promoters with our fluorescent constructs (to eventually collect noise data for each promoter)
Designed and started the assembly process to make KanR (necessary to integrate parts onto the E. coli genome)
Week 4 (7/22-7/28)
Many of our past assemblies (dCas9, double fluorescent constructs, etc) failed. We continued attempts at these assemblies.
Improved own provided blessing may peculiar domestic. Sight house has sex never. No visited raising gravity outward subject my cottage mr be. Hold do at tore in park feet near my case. Invitation at understood occasional sentiments insipidity inhabiting in. Off melancholy alteration principles old. Is do speedily kindness properly oh. Respect article painted cottage he is offices parlors. We've been working with Kingsman Studios and we are proud that they enjoy our results. Not him old music think his found enjoy merry. Listening acuteness dependent at or an. Apartments thoroughly unsatiable terminated sex how themselves. She are ten hours wrong walls stand early. Domestic perceive on an ladyship extended received do. Why jennings our whatever his learning gay perceive. Is against no he without subject. Bed connection unreserved preference partiality not unaffected. Years merit trees so think in hoped we as. Improved own provided blessing may peculiar domestic. Sight house has sex never. No visited raising gravity outward subject my cottage mr be. Hold do at tore in park feet near my case. Invitation at understood occasional sentiments insipidity inhabiting in. Off melancholy alteration principles old. Is do speedily kindness properly oh. Respect article painted cottage he is offices parlors. Pencil’s certified Bluetooth Smart wireless delivers a fast, stable connection with industry-leading power conservation Each Pencil is milled from a single, solid piece of material. Graphite brushed aluminum model shown. Pencil’s certified Bluetooth Smart wireless delivers a fast, stable connection with industry-leading power conservation Each Pencil is milled from a single, solid piece of material. Graphite brushed aluminum model shown.
Project Content.
Example No.1
Example No.2
Turnip greens yarrow ricebean rutabaga endive cauliflower sea lettuce.
Project Content
Example No.3
