Difference between revisions of "Team:SDU-Denmark/Tour53"

 
(43 intermediate revisions by 3 users not shown)
Line 1: Line 1:
{{:Team:SDU-Denmark/core/header1| }}
+
{{:Team:SDU-Denmark/core/header| }}
 
<html>
 
<html>
<div class="memberWrapper">
 
  
<h1 align="center"> Parts</h1>
+
<p> <i> "All in all it's just another brick in the wall" - <b>Pink Floyd</b></i></p>
<p> Below mentioned are the submitted parts. To explore sequencing, characterization etc. click the links and you will be directed to the parts registry page. </p>
+
<div class="accordion" style="width:90%; align: center">
+
  
  <div class="accordionTitel">Basic parts</div>
+
<h1 align="center" style="opacity: 1;"> Submitted Parts</h1>
  <div class="pane" style="height:50px;" >
+
<p><b>Below are our submitted parts.</b> If you want to dig deeper and explore sequencing, characterization etc. click the links and you will be directed to the parts registry page.</p>
  
  </div>
+
<br>
  
 +
<div class="accordion"  style="width:100%;">
  
   <div class="accordionTitel">Composite parts</div>
+
   <div class="accordionTitel">Standard Two-hybrid System Devices</div>
   <div class="pane" style="height:50px;" >
+
   <div class="pane current" style="height:360px;" >
  
 +
<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1638005" target="_blank">BBa_K1638005</a></span><br>
 +
<span class="intro">Plac-T18:</span>
 +
The T18 domain of <i>cyaA</i> with a <i>lac</i> promoter. Intented for use in the bacterial two-hybrid system, where a protein of interest can be fused to the C-terminal. The bacterial two-hybrid system allows detection of protein-protein interactions.
 +
 +
<br><br>
 +
 +
<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1638003" target="_blank">BBa_K1638003</a></span><br>
 +
<span class="intro">Plac-T25:</span>
 +
The T25 domain of <i>cyaA</i> with a <i>lac</i> promoter. Intented for use in the bacterial two-hybrid system, where a protein of interest can be fused to the C-terminal. The bacterial two-hybrid system allows detection of protein-protein interactions.
 +
 +
<br><br>
 +
 +
<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1638032" target="_blank">BBa_K1638032</a></span><br>
 +
<span class="intro">PcstA-RFP-term-Plac-T18:</span>
 +
The T18 domain of <i>cyaA</i> with a <i>lac</i> promoter. Intented for use in the bacterial two-hybrid system, where a protein of interest can be fused to the C-terminal. The bacterial two-hybrid system allows detection of protein-protein interactions. The cAMP-induced promoter PcstA induce transcription of RFP when a protein-protein interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.
 +
 +
<br><br>
 +
 +
<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1638033" target="_blank">BBa_K1638033</a></span><br>
 +
<span class="intro">PcstA-RFP-term-Plac-T25:</span>
 +
The T25 domain of <i>cyaA</i>A with a <i>lac</i> promoter. Intented for use in the bacterial two-hybrid system, where a protein of interest can be fused to the C-terminal. The bacterial two-hybrid system allows detection of protein-protein interactions. The cAMP-induced promoter PcstA induce transcription of RFP when a protein-protein interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.
  
 
   </div>
 
   </div>
  
  
   <div class="accordionTitel">Devices</div>
+
   <div class="accordionTitel">Control Devices</div>
   <div class="pane current" style="height:50px;" >
+
   <div class="pane" style="height:355px;" >
  
 +
<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1638011" target="_blank">BBa_K1638011</a></span><br>
 +
<span class="intro">Plac-T18-Zip:</span>
 +
Leucine Zipper fused to the T18 domain of <i>cyaA</i>. The expression of the gene is under control of a <i>lac</i> promoter. This part can be used to validate the bacterial two-hybrid system as leucine zippers is known to form a homodimer.
 +
 +
<br><br>
 +
 +
<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1638013" target="_blank">BBa_K1638013</a></span><br>
 +
<span class="intro">Plac-T25-Zip:</span>
 +
Leucine Zipper fused to the T25 domain of <i>cyaA</i>. The expression of the gene is under control of a <i>lac</i> promoter. This part can be used to validate the bacterial two-hybrid system as leucine zippers is known to form a homodimer.
 +
 +
<br><br>
 +
 +
<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1638031" target="_blank">BBa_K1638031</a></span><br>
 +
<span class="intro">PcstA-RFP-term-Plac-T18-Zip:</span>
 +
Leucine Zipper fused to the T18 domain of <i>cyaA</i>. The expression of the gene is under control of a <i>lac</i> promoter. This part can be used to validate the bacterial two-hybrid system as leucine zippers is known to form a homodimer. The cAMP-induced promoter PcstA induce transcription of RFP when a positive interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.
 +
 +
<br><br>
 +
 +
<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1638030" target="_blank">BBa_K1638030</a></span><br>
 +
<span class="intro">PcstA-RFP-term-Plac-T25-Zip:</span>
 +
Leucine Zipper fused to the T25 domain of <i>cyaA</i>. The expression of the gene is under control of a <i>lac</i> promoter. This part can be used to validate the bacterial two-hybrid system as leucine zippers is known to form a homodimer. The cAMP-induced promoter PcstA induce transcription of RFP when a positive interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.
  
 
   </div>
 
   </div>
  
  
</div>
+
  <div class="accordionTitel">Screening Devices</div>
 +
  <div class="pane" style="height:570px;" >
  
 +
<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1638018" target="_blank">BBa_K1638018</a></span><br>
 +
<span class="intro">PLlacO-1-T18-linker-hTrx_scaffold-(3xFlag):</span>
 +
The human thioredoxin scaffold fused to the T18 domain of <i>cyaA</i> through a flexible linker. The human thioredoxin scaffold is a scaffold used for presentation of peptide aptamers. When a random nucleotide library is inserted into the scaffold, one can use the bacterial two-hybrid system to screen against specific targets. An affinity 3xFlag-tag is fused to the C-terminal for detection purposes. The part is under control of the hybrid PLlacO-1 promoter.
  
 +
<br><br>
  
<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
+
<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1638035" target="_blank">BBa_K1638035</a></span><br>
<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
+
<span class="intro">Plac-T18-linker-Intein-hTrx_scaffold-(3xFlag):</span>
 +
The human thioredoxin scaffold fused to the T18 domain of <i>cyaA</i> through intein and a flexible linker. The human thioredoxin scaffold is a scaffold used for presentation of peptide aptamers. When a random nucleotide library is inserted into the scaffold, one can use the bacterial two-hybrid system to screen against specific targets. The intein is added for affinity purification purposes. This allows us to only purify the pure hTrx-based peptide aptamer. An affinity 3xFlag-tag is fused to the C-terminal for detection purposes.
  
 +
<br><br>
  
<div class="highlightBox">
+
<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1638037" target="_blank">BBa_K1638037</a></span><br>
<h4>Note</h4>
+
<span class="intro">PcstA-RFP-term-Plac-T25-linker-CsrA:</span>
<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
+
The carbon storage regulator CsrA fused to the T25 domain of the adenylate cyclase, CyaA. Made for the purpose to be a 'bait' in a peptide-aptamer screening using the hTrx-based peptide aptamer fused to the T18 domain of CyaA. The cAMP-induced promoter PcstA induce transcription of RFP when a positive interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.
</div>
+
  
 +
<br><br>
  
 +
<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1638038" target="_blank">BBa_K1638038</a></span><br>
 +
<span class="intro">PcstA-RFP-term-Plac-T25-linker-YhbJ:</span>
 +
The RNase adapter protein YhbJ fused to the T25 domain of the adenylate cyclase, CyaA. Made for the purpose to be a 'bait' in a peptide-aptamer screening using the hTrx-based peptide aptamer fused to the T18 domain of CyaA. The cAMP-induced promoter PcstA induce transcription of RFP when a positive interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.
  
<h4>Adding parts to the registry</h4>
+
<br><br>
<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
+
<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
+
  
 +
<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1638039" target="_blank">BBa_K1638039</a></span><br>
 +
<span class="intro">PcstA-RFP-term-Plac-T25-linker-Hfq:</span>
 +
The RNA-binding protein Hfq fused to the T25 domain of the adenylate cyclase, CyaA. Made for the purpose to be a 'bait' in a peptide-aptamer screening using the hTrx-based peptide aptamer fused to the T18 domain of CyaA. The cAMP-induced promoter PcstA induce transcription of RFP when a positive interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.
  
<h4>What information do I need to start putting my parts on the Registry?</h4>
+
  </div>
<p>The information needed to initially create a part on the Registry is:</p>
+
<ul>
+
<li>Part Name</li>
+
<li>Part type</li>
+
<li>Creator</li>
+
<li>Sequence</li>
+
<li>Short Description (60 characters on what the DNA does)</li>
+
<li>Long Description (Longer description of what the DNA does)</li>
+
<li>Design considerations</li>
+
</ul>
+
  
<p>
 
We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
 
  
 +
  <div class="accordionTitel">Reporter Fusions</div>
 +
  <div class="pane" style="height:205px;" >
  
<h4>Inspiration</h4>
+
<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1638009" target="_blank">BBa_K1638009</a></span><br>
<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
+
<span class="intro">Plac-T18-linker-GFP:</span>
 +
Green Fluorescent Protein (GFP) fused to the T18 domain of the adenylate cyclase CyaA through a flexible linker. This part is used to check gene expression and validate that T18-fusion proteins will fold correctly.
 +
<br><br>
  
 +
<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1638010" target="_blank">BBa_K1638010</a></span><br>
 +
<span class="intro">Plac-T25-linker-GFP:</span>
 +
Green Fluorescent Protein (GFP) fused to the T25 domain of the adenylate cyclase CyaA through a flexible linker. This part is used to check gene expression and validate that T25-fusion proteins will fold correctly.
  
 +
<br><br>
 +
 +
<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1638007" target="_blank">BBa_K1638007</a></span><br>
 +
<span class="intro">linker-GFP:</span>
 +
A flexible 10 amino acid linker fused to the N-terminal part of GFP. Intended for use in protein-GFP fusions, where this part can be suffixed to various proteins as a reporter of gene expression.
 +
 +
  </div>
 +
 +
 +
  <div class="accordionTitel">Others</div>
 +
  <div class="pane" style="height:70px;" >
 +
 +
<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1638036" target="_blank">BBa_K1638036</a></span><br>
 +
<span class="intro">PLlacO-1-Intein:</span>
 +
This part consist of an intein from <i>Saccharomyces cerevisiae</i> <i>VMA1</i> gene including a CBD-tag. This intein is a thiol-induced self-cleavable protein that enables the release of a C-terminal fused protein. The intein is under control of the hybrid PLlacO-1 promoter.
 +
 +
  </div>
  
  

Latest revision as of 02:47, 19 September 2015

"All in all it's just another brick in the wall" - Pink Floyd

Submitted Parts

Below are our submitted parts. If you want to dig deeper and explore sequencing, characterization etc. click the links and you will be directed to the parts registry page.


Standard Two-hybrid System Devices
BBa_K1638005
Plac-T18: The T18 domain of cyaA with a lac promoter. Intented for use in the bacterial two-hybrid system, where a protein of interest can be fused to the C-terminal. The bacterial two-hybrid system allows detection of protein-protein interactions.

BBa_K1638003
Plac-T25: The T25 domain of cyaA with a lac promoter. Intented for use in the bacterial two-hybrid system, where a protein of interest can be fused to the C-terminal. The bacterial two-hybrid system allows detection of protein-protein interactions.

BBa_K1638032
PcstA-RFP-term-Plac-T18: The T18 domain of cyaA with a lac promoter. Intented for use in the bacterial two-hybrid system, where a protein of interest can be fused to the C-terminal. The bacterial two-hybrid system allows detection of protein-protein interactions. The cAMP-induced promoter PcstA induce transcription of RFP when a protein-protein interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.

BBa_K1638033
PcstA-RFP-term-Plac-T25: The T25 domain of cyaAA with a lac promoter. Intented for use in the bacterial two-hybrid system, where a protein of interest can be fused to the C-terminal. The bacterial two-hybrid system allows detection of protein-protein interactions. The cAMP-induced promoter PcstA induce transcription of RFP when a protein-protein interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.
Control Devices
BBa_K1638011
Plac-T18-Zip: Leucine Zipper fused to the T18 domain of cyaA. The expression of the gene is under control of a lac promoter. This part can be used to validate the bacterial two-hybrid system as leucine zippers is known to form a homodimer.

BBa_K1638013
Plac-T25-Zip: Leucine Zipper fused to the T25 domain of cyaA. The expression of the gene is under control of a lac promoter. This part can be used to validate the bacterial two-hybrid system as leucine zippers is known to form a homodimer.

BBa_K1638031
PcstA-RFP-term-Plac-T18-Zip: Leucine Zipper fused to the T18 domain of cyaA. The expression of the gene is under control of a lac promoter. This part can be used to validate the bacterial two-hybrid system as leucine zippers is known to form a homodimer. The cAMP-induced promoter PcstA induce transcription of RFP when a positive interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.

BBa_K1638030
PcstA-RFP-term-Plac-T25-Zip: Leucine Zipper fused to the T25 domain of cyaA. The expression of the gene is under control of a lac promoter. This part can be used to validate the bacterial two-hybrid system as leucine zippers is known to form a homodimer. The cAMP-induced promoter PcstA induce transcription of RFP when a positive interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.
Screening Devices
BBa_K1638018
PLlacO-1-T18-linker-hTrx_scaffold-(3xFlag): The human thioredoxin scaffold fused to the T18 domain of cyaA through a flexible linker. The human thioredoxin scaffold is a scaffold used for presentation of peptide aptamers. When a random nucleotide library is inserted into the scaffold, one can use the bacterial two-hybrid system to screen against specific targets. An affinity 3xFlag-tag is fused to the C-terminal for detection purposes. The part is under control of the hybrid PLlacO-1 promoter.

BBa_K1638035
Plac-T18-linker-Intein-hTrx_scaffold-(3xFlag): The human thioredoxin scaffold fused to the T18 domain of cyaA through intein and a flexible linker. The human thioredoxin scaffold is a scaffold used for presentation of peptide aptamers. When a random nucleotide library is inserted into the scaffold, one can use the bacterial two-hybrid system to screen against specific targets. The intein is added for affinity purification purposes. This allows us to only purify the pure hTrx-based peptide aptamer. An affinity 3xFlag-tag is fused to the C-terminal for detection purposes.

BBa_K1638037
PcstA-RFP-term-Plac-T25-linker-CsrA: The carbon storage regulator CsrA fused to the T25 domain of the adenylate cyclase, CyaA. Made for the purpose to be a 'bait' in a peptide-aptamer screening using the hTrx-based peptide aptamer fused to the T18 domain of CyaA. The cAMP-induced promoter PcstA induce transcription of RFP when a positive interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.

BBa_K1638038
PcstA-RFP-term-Plac-T25-linker-YhbJ: The RNase adapter protein YhbJ fused to the T25 domain of the adenylate cyclase, CyaA. Made for the purpose to be a 'bait' in a peptide-aptamer screening using the hTrx-based peptide aptamer fused to the T18 domain of CyaA. The cAMP-induced promoter PcstA induce transcription of RFP when a positive interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.

BBa_K1638039
PcstA-RFP-term-Plac-T25-linker-Hfq: The RNA-binding protein Hfq fused to the T25 domain of the adenylate cyclase, CyaA. Made for the purpose to be a 'bait' in a peptide-aptamer screening using the hTrx-based peptide aptamer fused to the T18 domain of CyaA. The cAMP-induced promoter PcstA induce transcription of RFP when a positive interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.
Reporter Fusions
BBa_K1638009
Plac-T18-linker-GFP: Green Fluorescent Protein (GFP) fused to the T18 domain of the adenylate cyclase CyaA through a flexible linker. This part is used to check gene expression and validate that T18-fusion proteins will fold correctly.

BBa_K1638010
Plac-T25-linker-GFP: Green Fluorescent Protein (GFP) fused to the T25 domain of the adenylate cyclase CyaA through a flexible linker. This part is used to check gene expression and validate that T25-fusion proteins will fold correctly.

BBa_K1638007
linker-GFP: A flexible 10 amino acid linker fused to the N-terminal part of GFP. Intended for use in protein-GFP fusions, where this part can be suffixed to various proteins as a reporter of gene expression.
Others
BBa_K1638036
PLlacO-1-Intein: This part consist of an intein from Saccharomyces cerevisiae VMA1 gene including a CBD-tag. This intein is a thiol-induced self-cleavable protein that enables the release of a C-terminal fused protein. The intein is under control of the hybrid PLlacO-1 promoter.