Difference between revisions of "Team:NJAU China/Parts"

 
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}
 
}
 
h2{
 
h2{
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background:url(../images/NJAU_China2015_frog.png) no-repeat;
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background:url(https://static.igem.org/mediawiki/2015/b/b3/NJAU_China2015_frog.png) no-repeat;
 
}
 
}
 
.section img{
 
.section img{
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height:200px;
 
height:200px;
 
display:block;
 
display:block;
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}
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table,td{
 +
border:2px solid #69C;
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border-collapse:collapse;
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text-align:center;
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}
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td{
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padding:0 10px 0 10px;
 
}
 
}
 
</style>
 
</style>
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<div class="main_content">
 
<div class="main_content">
 
<div class="section">
 
<div class="section">
 +
 +
<table>
 +
<tr>
 +
<td><p>Label</p></td>
 +
<td><p>Name</p></td>
 +
<td><p>Type</p></td>
 +
<td><p>Description</p></td>
 +
<td><p>Designer</p></td>
 +
<td><p>Length</p></td>
 +
</tr>
 +
<tr>
 +
<td></td>
 +
<td><p><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1649000">BBa_K1649000</a></p></td>
 +
<td><p>Composite</p></td>
 +
<td><p>red light sensor</p></td>
 +
<td><p>Yang Yang</p></td>
 +
<td><p>4000bp</p></td>
 +
</tr>
 +
<tr>
 +
<td></td>
 +
<td><p><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1649001">BBa_K1649001</a></p></td>
 +
<td><p>Composite</p></td>
 +
<td><p>red light sensor with a GFP reporter </p></td>
 +
<td><p>Yang Yang</p></td>
 +
<td><p>4883bp</p></td>
 +
</tr>
 +
<tr>
 +
<td><p>Favorite</p></td>
 +
<td><p><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1649002">BBa_K1649002</a></p></td>
 +
<td><p>Composite</p></td>
 +
<td><p>Red light sensor with TetR not gate and GF<a name="_GoBack"></a>P reporter </p></td>
 +
<td><p>Yang Yang</p></td>
 +
<td><p>7280bp</p></td>
 +
</tr>
 +
<tr>
 +
<td><p>Favorite</p></td>
 +
<td><p><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1649003">BBa_K1649003</a></p></td>
 +
<td><p>Composite</p></td>
 +
<td><p>YF1 blue light sensor with GFP reporter </p></td>
 +
<td><p>Yang Yang</p></td>
 +
<td><p>3117bp</p></td>
 +
</tr>
 +
<tr>
 +
<td></td>
 +
<td><p><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1649004">BBa_K1649004</a><u> </u></p></td>
 +
<td ><p>Composite</p></td>
 +
<td><p>YF1-FixJ with terminator </p></td>
 +
<td><p>Yang Yang</p></td>
 +
<td><p>1933bp</p></td>
 +
</tr>
 +
</table>
 +
 
<h2>BBa_K1649000</h2>
 
<h2>BBa_K1649000</h2>
−
<p>red light sensor</p>
+
<p>Red Light Sensor</p>
 
<div class="first_down"><span>T</span>
 
<div class="first_down"><span>T</span>
 
<p>he chimaera Cph8 is the phytochrome Cph1 fusing with a cyanobacterial photoreceptor EnvZ which can engineer a red light-regulated transcription system in E. coli, with the phycocyanobilin-biosynthesis genes (ho1 and pcyA). The promoter OmpC is repressed by the function of red light and activated by dark.</p>
 
<p>he chimaera Cph8 is the phytochrome Cph1 fusing with a cyanobacterial photoreceptor EnvZ which can engineer a red light-regulated transcription system in E. coli, with the phycocyanobilin-biosynthesis genes (ho1 and pcyA). The promoter OmpC is repressed by the function of red light and activated by dark.</p>
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<img src="https://static.igem.org/mediawiki/2015/f/f0/NJAU_2015_teamparts1.png"/>
 
<img src="https://static.igem.org/mediawiki/2015/f/f0/NJAU_2015_teamparts1.png"/>
 
<h2>BBa_K1649001</h2>
 
<h2>BBa_K1649001</h2>
−
<p>red light sensor with a GFP reporter</p>
+
<p>Red Light Sensor with a GFP Reporter</p>
 
<div class="first_down"><span class="first_down">G</span>
 
<div class="first_down"><span class="first_down">G</span>
 
<p>FP can be used to evaluate the efficiency of red light sensor when this part is regulated by different promoters by measuring the florescence.</p>
 
<p>FP can be used to evaluate the efficiency of red light sensor when this part is regulated by different promoters by measuring the florescence.</p>
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<h2>BBa_K1649002</h2>
 
<h2>BBa_K1649002</h2>
−
<p>Red light sensor with TetR not gate and GFP reporter</p>
+
<p>Red Light Sensor with TetR Not Gate and GFP Reporter</p>
 
<div class="first_down"><span class="first_down">A</span>
 
<div class="first_down"><span class="first_down">A</span>
 
<p>s the transcription from the red light sensor is repressed by the function of red light and activated by dark, we use a not gate, repressor TetR to ensure the system is activated by red light. GFP can be used to test whether the circuit works.</p>
 
<p>s the transcription from the red light sensor is repressed by the function of red light and activated by dark, we use a not gate, repressor TetR to ensure the system is activated by red light. GFP can be used to test whether the circuit works.</p>
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<h2>BBa_K1649004</h2>
 
<h2>BBa_K1649004</h2>
−
<p>YF1-FixJ with terminator</p>
+
<p>YF1-FixJ with Terminator</p>
 
<div class="first_down"><span class="first_down">T</span>
 
<div class="first_down"><span class="first_down">T</span>
 
<p>he LOV photo-switch YF1 was created by fusing the light perception domain of YtvA to the FixL kinase domain. YtvA acts as a blue-light photoreceptor and carries a blue-light-sensitive flavin-binding LOV domain. The response regulator FixJ forms a two-component system together with FixL. The sensor is inactive in light and active in the dark.</p>
 
<p>he LOV photo-switch YF1 was created by fusing the light perception domain of YtvA to the FixL kinase domain. YtvA acts as a blue-light photoreceptor and carries a blue-light-sensitive flavin-binding LOV domain. The response regulator FixJ forms a two-component system together with FixL. The sensor is inactive in light and active in the dark.</p>
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<h2>BBa_K1649003</h2>
 
<h2>BBa_K1649003</h2>
−
<p>YF1 blue light sensor with GFP reporter</p>
+
<p>YF1 Blue Light Sensor with GFP Reporter</p>
 
<div class="first_down"><span class="first_down">G</span>
 
<div class="first_down"><span class="first_down">G</span>
 
<p>FP is used to report the function of blue light sensor.</p>
 
<p>FP is used to report the function of blue light sensor.</p>

Latest revision as of 03:23, 19 September 2015

Label

Name

Type

Description

Designer

Length

BBa_K1649000

Composite

red light sensor

Yang Yang

4000bp

BBa_K1649001

Composite

red light sensor with a GFP reporter

Yang Yang

4883bp

Favorite

BBa_K1649002

Composite

Red light sensor with TetR not gate and GFP reporter

Yang Yang

7280bp

Favorite

BBa_K1649003

Composite

YF1 blue light sensor with GFP reporter

Yang Yang

3117bp

BBa_K1649004

Composite

YF1-FixJ with terminator

Yang Yang

1933bp

BBa_K1649000

Red Light Sensor

T

he chimaera Cph8 is the phytochrome Cph1 fusing with a cyanobacterial photoreceptor EnvZ which can engineer a red light-regulated transcription system in E. coli, with the phycocyanobilin-biosynthesis genes (ho1 and pcyA). The promoter OmpC is repressed by the function of red light and activated by dark.

BBa_K1649001

Red Light Sensor with a GFP Reporter

G

FP can be used to evaluate the efficiency of red light sensor when this part is regulated by different promoters by measuring the florescence.

BBa_K1649002

Red Light Sensor with TetR Not Gate and GFP Reporter

A

s the transcription from the red light sensor is repressed by the function of red light and activated by dark, we use a not gate, repressor TetR to ensure the system is activated by red light. GFP can be used to test whether the circuit works.

BBa_K1649004

YF1-FixJ with Terminator

T

he LOV photo-switch YF1 was created by fusing the light perception domain of YtvA to the FixL kinase domain. YtvA acts as a blue-light photoreceptor and carries a blue-light-sensitive flavin-binding LOV domain. The response regulator FixJ forms a two-component system together with FixL. The sensor is inactive in light and active in the dark.

BBa_K1649003

YF1 Blue Light Sensor with GFP Reporter

G

FP is used to report the function of blue light sensor.