Difference between revisions of "Team:Amoy/Interlab"
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<a href="https://2015.igem.org/Team:Amoy/Interlab"><h4>Interlab</h4></a> | <a href="https://2015.igem.org/Team:Amoy/Interlab"><h4>Interlab</h4></a> | ||
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− | <div id="title" | + | <div id="title" style="width: 70%; margin-left: 25%;"> |
<p id="title_p">INTERLAB</p> | <p id="title_p">INTERLAB</p> | ||
− | <h1 id="Introduction" class="main_h1"> | + | <h1 id="Introduction" class="main_h1">Ⅰ. Introduction</h1> |
<p class="main_p">The goal of the Interlab study is to obtain fluorescence data for three specific genetic devices expressing GFP from iGEM teams around the world. This year, three devices were cloned by ourselves, and one positive control and one negative control were provided by the registry. Using a plate reader, fluorescence measurements were obtained in arbitrary units. The results show increased fluorescence in the stronger promoter expected.</br> | <p class="main_p">The goal of the Interlab study is to obtain fluorescence data for three specific genetic devices expressing GFP from iGEM teams around the world. This year, three devices were cloned by ourselves, and one positive control and one negative control were provided by the registry. Using a plate reader, fluorescence measurements were obtained in arbitrary units. The results show increased fluorescence in the stronger promoter expected.</br> | ||
</br> | </br> | ||
After the experiment, three required devices were created: </br> | After the experiment, three required devices were created: </br> | ||
− | J23101+I13504 in the | + | J23101+I13504 in the pSB3C3 backbone. </br> |
J23106+I13504 in the pSB1C3 backbone.</br> | J23106+I13504 in the pSB1C3 backbone.</br> | ||
J23117+I13504 in the pSB1C3 backbone. </br> | J23117+I13504 in the pSB1C3 backbone. </br> | ||
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</p> | </p> | ||
− | <img class="main_img" src="https://static.igem.org/mediawiki/2015/ | + | <img class="main_img" src="https://static.igem.org/mediawiki/2015/1/1c/Amoy-Interlab-pic1.png" /> |
− | <p class="figure">Figure 1. GFP generator with different promoters | + | <p class="figure" style="width: 450px;"><strong>Figure 1.</strong> GFP generator with different promoters.</br> |
1. J23101+I13504 in DH5α;</br> | 1. J23101+I13504 in DH5α;</br> | ||
2. J23106+I13504 in DH5α;</br> | 2. J23106+I13504 in DH5α;</br> | ||
− | 3. J23117+I13504 in DH5α.</p> | + | 3. J23117+I13504 in DH5α.</br></p> |
− | <img class="main_img" src="https://static.igem.org/mediawiki/2015/ | + | <img class="main_img" src="https://static.igem.org/mediawiki/2015/a/aa/Amoy-Interlab-pic2.png" /> |
− | <p class="figure">Figure 2. Centrifugation of bacteria.</br> | + | <p class="figure" style="width: 450px;"><strong>Figure 2.</strong> Centrifugation of bacteria.</br> |
1. J23101+I13504;</br> | 1. J23101+I13504;</br> | ||
2. J23106+I13504;</br> | 2. J23106+I13504;</br> | ||
3. J23117+I13504;</br> | 3. J23117+I13504;</br> | ||
4. Positive control (I20270);</br> | 4. Positive control (I20270);</br> | ||
− | 5. Negative control (R0040 in pSB1C3)</p> | + | 5. Negative control (R0040 in pSB1C3)</br></p> |
− | <h2 class="main_h2">Digestion Verification</h2> | + | <h2 class="main_h2">1. Digestion Verification</h2> |
− | <p class="main_p">Four devices (J23101+I13504/J23106+I13504/J23117+I13504/I20270) are double digested at <i>EcoR I</i> and <i>Pst I</i> restriction sites to verify the target parts. The restriction map is shown as Figure 3. As expected, the second bands are supposed to be aroud 1000bp, which is consistent with what we saw on the map. </p> | + | <p class="main_p">Four devices (J23101+I13504/J23106+I13504/J23117+I13504/I20270) are double digested at <i>EcoR I</i> and <i>Pst I</i> restriction sites to verify the target parts. The restriction map is shown as Figure 3. As expected, the second bands are supposed to be aroud 1000bp, which is consistent with what we saw on the map. </br></p> |
− | <img class="main_img" src="https://static.igem.org/mediawiki/2015/ | + | <img class="main_img" src="https://static.igem.org/mediawiki/2015/6/68/Amoy-Interlab-pic3.png" /> |
− | <p class="figure">Figure 3. Restriction map of digestion verification. 2000plus DNA marker.</ | + | <p class="figure" style="width: 450px;"><strong>Figure 3.</strong> Restriction map of digestion verification. 2000plus DNA marker.</br> |
− | + | 1. Double digestion of J23101+I13504 (constructed plasmid)</br> | |
− | + | ||
2. Double digestion of J23106+I13504 (constructed plasmid)</br> | 2. Double digestion of J23106+I13504 (constructed plasmid)</br> | ||
3. Double digestion of J23117+I13504 (constructed plasmid)</br> | 3. Double digestion of J23117+I13504 (constructed plasmid)</br> | ||
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</p> | </p> | ||
− | <h2 class="main_h2">DNA sequencing</h2> | + | <h2 class="main_h2">2. DNA sequencing</h2> |
− | <p class="main_p">All created parts are verified by DNA sequencing, as | + | <p class="main_p">All created parts are verified by DNA sequencing, analyzed by DNAMAN software, as following:</br></p> |
− | + | <table align="center" width="600px" height="40px"> | |
− | < | + | <tbody> |
− | < | + | <tr class="firstRow"> |
+ | <td width="424" valign="middle" style="word-break: break-all;" align="center"> | ||
+ | <span style="font-size: 18px;"><a href="https://static.igem.org/mediawiki/2015/d/d8/Amoy_J23101%2BI13504.txt">J23101+I13504.txt</a></span> | ||
+ | </td> | ||
+ | <td width="424" valign="middle" style="word-break: break-all;" align="center"> | ||
+ | <span style="font-size: 18px;"><a href="https://static.igem.org/mediawiki/2015/0/04/Amoy_J23106%2BI13504.txt">J23106+I13504.txt</a></span> | ||
+ | </td> | ||
+ | <td width="424" valign="middle" style="word-break: break-all;" align="center"> | ||
+ | <span style="font-size: 18px;"><a href="https://static.igem.org/mediawiki/2015/6/65/Amoy_J23117%2BI13504.txt">J23117+I13504.txt</a></span> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
− | <h1 id="Protocol" class="main_h1"> | + | <h1 id="Protocol" class="main_h1">Ⅱ. Protocol</h1> |
<p class="main_p">1. Transform constructed plasmids into DH5α competent cells, grown in incubator for 12 hrs at 37℃.</br> | <p class="main_p">1. Transform constructed plasmids into DH5α competent cells, grown in incubator for 12 hrs at 37℃.</br> | ||
2. Add 5 mL LB medium with antibiotic (Chloramphenicol 35 μg/mL) into test tubes, choose monoclonal cells from the petri dish.</br> | 2. Add 5 mL LB medium with antibiotic (Chloramphenicol 35 μg/mL) into test tubes, choose monoclonal cells from the petri dish.</br> | ||
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Device: Spectrophotometer </br> | Device: Spectrophotometer </br> | ||
Wavelengths: 600 nm absorption.</br> | Wavelengths: 600 nm absorption.</br> | ||
− | |||
(2) Fluorescence</br> | (2) Fluorescence</br> | ||
Device: FLUOstar omega microplate reader, 96-well plates.</br> | Device: FLUOstar omega microplate reader, 96-well plates.</br> | ||
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</p> | </p> | ||
− | <h1 id="Measurements" class="main_h1"> | + | <h1 id="Measurements" class="main_h1">Ⅲ. Measurements</h1> |
<p class="main_p">Absorbance at 600 nm was measured for each of the three cultures, alongside controls for media and contamination background. The dilution required for each sample has been calculated. Table 1 presents the re-measurement of samples on OD 600, and all OD 600 are within 5% of 0.5 (0.475-0.525).</p> | <p class="main_p">Absorbance at 600 nm was measured for each of the three cultures, alongside controls for media and contamination background. The dilution required for each sample has been calculated. Table 1 presents the re-measurement of samples on OD 600, and all OD 600 are within 5% of 0.5 (0.475-0.525).</p> | ||
− | <table align="center" width=" | + | <table align="center" width="850px" height="240px;"> |
− | <caption style="border-color: rgb(221, 221, 221);"> | + | <caption style="border-color: rgb(221, 221, 221); text-align: center; vertical-align: middle;"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">Table 1. Absorbance at 600 nm for 3 required devices read in spectrophotometer. </span> |
</caption> | </caption> | ||
<tbody> | <tbody> | ||
<tr class="firstRow"> | <tr class="firstRow"> | ||
− | <td style=" | + | <td align="center" valign="middle" colspan="1" rowspan="1" style="border: 1px solid rgb(221, 221, 221);" width="60"></td> |
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" colspan="1" rowspan="1" style="border: 1px solid rgb(221, 221, 221);" width="243"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">J23101+I13504</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" colspan="1" rowspan="1" style="border: 1px solid rgb(221, 221, 221);" width="244"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">J23106+I13504</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" colspan="1" rowspan="1" style="border: 1px solid rgb(221, 221, 221);" width="244"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">J23117+I13504</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" colspan="1" rowspan="1" style="border: 1px solid rgb(221, 221, 221);" width="163"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">Negative </span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" colspan="1" rowspan="1" style="border: 1px solid rgb(221, 221, 221);" width="150"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">Positive </span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" colspan="1" rowspan="1" style="border: 1px solid rgb(221, 221, 221);" width="214"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">LB+Antibiotic</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td style="word-break: break-all; border: 1px solid rgb(221, 221, 221);" align="center" valign="middle" width="60"></td> |
− | <span style="font-size: | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="243"> |
+ | <span style="font-size: 18px;">A</span> | ||
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221 | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="244"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">B</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221 | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="244"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">C</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221 | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="163"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">D</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(235, 241, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="150"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">E</span> |
+ | </td> | ||
+ | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="214"> | ||
+ | <span style="font-size: 18px;">F</span> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="60"> | ||
+ | <span style="font-size: 18px;">1</span> | ||
+ | </td> | ||
+ | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(253, 234, 218);" width="243"> | ||
+ | <span style="font-size: 18px;">0.509</span> | ||
+ | </td> | ||
+ | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(219, 238, 243);" width="244"> | ||
+ | <span style="font-size: 18px;">0.500</span> | ||
+ | </td> | ||
+ | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(229, 224, 236);" width="244"> | ||
+ | <span style="font-size: 18px;">0.502</span> | ||
+ | </td> | ||
+ | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(235, 241, 221);" width="163"> | ||
+ | <span style="font-size: 18px;">0.484</span> | ||
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(242, 220, 219);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(242, 220, 219);" width="150"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">0.479</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(219, 229, 241);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(219, 229, 241);" width="214"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">0.089</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="60"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">2</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(253, 234, 218);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(253, 234, 218);" width="243"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">0.499</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(219, 238, 243);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(219, 238, 243);" width="244"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">0.500</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(229, 224, 236);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(229, 224, 236);" width="244"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">0.507</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(235, 241, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(235, 241, 221);" width="163"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">0.519</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(242, 220, 219);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(242, 220, 219);" width="150"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">0.497</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(219, 229, 241);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(219, 229, 241);" width="214"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">0.074</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="60"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">3</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(253, 234, 218);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(253, 234, 218);" width="243"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">0.505</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(219, 238, 243);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(219, 238, 243);" width="244"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">0.497</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(229, 224, 236);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(229, 224, 236);" width="244"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">0.503</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(235, 241, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(235, 241, 221);" width="163"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">0.483</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(242, 220, 219);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(242, 220, 219);" width="150"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">0.509</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(219, 229, 241);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(219, 229, 241);" width="214"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">0.080</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="60"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">G</span> |
+ | </td> | ||
+ | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="243"> | ||
+ | <span style="font-size: 18px;">/</span> | ||
+ | </td> | ||
+ | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="244"> | ||
+ | <span style="font-size: 18px;">/</span> | ||
+ | </td> | ||
+ | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="244"> | ||
+ | <span style="font-size: 18px;">/</span> | ||
+ | </td> | ||
+ | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="163"> | ||
+ | <span style="font-size: 18px;">/</span> | ||
+ | </td> | ||
+ | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="150"> | ||
+ | <span style="font-size: 18px;">/</span> | ||
+ | </td> | ||
+ | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="214"> | ||
+ | <span style="font-size: 18px;">/</span> | ||
</td> | </td> | ||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
</tr> | </tr> | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
− | + | </br> | |
− | <p class=" | + | <p class="main_p"> |
− | + | D wells=negative control (R0040 in pSB1C3 backbone);</br> | |
− | + | E wells=positive control (I20270 in pSB1C3 backbone);</br> | |
− | + | F wells=LB media plus antibiotic (chloramphenicol only);</br> | |
− | + | G wells=Non-inoculate culture; | |
− | + | </p><hr> | |
− | + | ||
− | </p> | + | |
− | + | ||
<p class="main_p">Table 2 presents the Fluorescence we measured, using three biological replicates of each device, alongside controls for LB media plus antibiotic and background value. </p> | <p class="main_p">Table 2 presents the Fluorescence we measured, using three biological replicates of each device, alongside controls for LB media plus antibiotic and background value. </p> | ||
− | <table align="center" width="800px" height=" | + | <table class="mytable" align="center" width="800px" height="280px" data-sort="sortDisabled"> |
− | <caption style="border-color: rgb(221, 221, 221);"> | + | <caption style="border-color: rgb(221, 221, 221); text-align: center; vertical-align: middle;"> |
− | + | <span style="font-size: 18px;">Table 2a. Fluorescence for 3 devices read in a FLUOstar omega microplate reader.</span><br/> | |
− | . | + | |
</caption> | </caption> | ||
<tbody> | <tbody> | ||
<tr class="firstRow"> | <tr class="firstRow"> | ||
− | <td style=" | + | <td align="center" valign="middle" colspan="1" rowspan="1" style="border: 1px solid rgb(221, 221, 221);" width="194"></td> |
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" colspan="3" rowspan="1" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">J23101+I13504</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" colspan="3" rowspan="1" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">J23106+I13504</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" colspan="3" rowspan="1" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">J23117+I3504<br/></span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | </tr> |
− | <span style="font-size: | + | <tr> |
+ | <td style="word-break: break-all; border: 1px solid rgb(221, 221, 221);" align="center" valign="middle" width="131"></td> | ||
+ | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="131"> | ||
+ | <span style="font-size: 18px;">A1</span> | ||
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="131"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">A2</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="131"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">A3</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="113"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">B1</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="113"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">B2</span> |
+ | </td> | ||
+ | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="113"> | ||
+ | <span style="font-size: 18px;">B3</span> | ||
+ | </td> | ||
+ | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="194"> | ||
+ | <span style="font-size: 18px;">C1</span> | ||
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="130"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="130"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">C2</span> |
+ | </td> | ||
+ | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="131"> | ||
+ | <span style="font-size: 18px;">C3</span> | ||
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="131"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">Replicate1</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(253, 234, 218);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(253, 234, 218);" width="131"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">57850</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(253, 234, 218);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(253, 234, 218);" width="131"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">55881</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(253, 234, 218);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(253, 234, 218);" width="131"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">55501</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(219, 238, 243);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(219, 238, 243);" width="113"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">36054</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(219, 238, 243);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(219, 238, 243);" width="113"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">34549</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(219, 238, 243);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(219, 238, 243);" width="113"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">34799</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(229, 224, 236);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(229, 224, 236);" width="194"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">958</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(229, 224, 236);" width="130"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(229, 224, 236);" width="130"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">960</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(229, 224, 236);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(229, 224, 236);" width="131"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">957</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="131"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">Replicate2</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(253, 234, 218);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(253, 234, 218);" width="131"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">55682</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(253, 234, 218);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(253, 234, 218);" width="131"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">54154</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(253, 234, 218);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(253, 234, 218);" width="131"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">53233</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(219, 238, 243);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(219, 238, 243);" width="113"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">37165</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(219, 238, 243);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(219, 238, 243);" width="113"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">35969</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(219, 238, 243);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(219, 238, 243);" width="113"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">35697</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(229, 224, 236);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(229, 224, 236);" width="194"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">1098</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(229, 224, 236);" width="130"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(229, 224, 236);" width="130"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">1073</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(229, 224, 236);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(229, 224, 236);" width="131"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">1071</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="131"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">Replicate3</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(253, 234, 218);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(253, 234, 218);" width="131"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">56413</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(253, 234, 218);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(253, 234, 218);" width="131"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">54693</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(253, 234, 218);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(253, 234, 218);" width="131"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">53803</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(219, 238, 243);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(219, 238, 243);" width="113"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">33475</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(219, 238, 243);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(219, 238, 243);" width="113"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">31901</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(219, 238, 243);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(219, 238, 243);" width="113"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">31920</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(229, 224, 236);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(229, 224, 236);" width="194"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">1401</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(229, 224, 236);" width="130"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(229, 224, 236);" width="130"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">1381</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(229, 224, 236);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(229, 224, 236);" width="131"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">1375</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="131"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">F</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="131"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">6937</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="131"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">6892</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="131"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">7183</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="113"> |
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="113"> |
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="113"> |
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="194"> |
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="130"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="130"> | ||
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="131"> |
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="131"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">G</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="131"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">114</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="131"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">108</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="131"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">105</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="113"> |
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="113"> |
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="113"> |
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="undefined"> |
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="undefined"> |
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="undefined"> |
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
</tr> | </tr> | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
− | + | ||
− | + | <table class="mytable" width="600px" align="center" height="280px" data-sort="sortDisabled"> | |
− | + | <caption style="border-color: rgb(221, 221, 221); text-align: center; vertical-align: middle;"> | |
− | <table width="600px" align="center" height=" | + | <span style="font-size: 18px;">Table 2b. Fluorescence of negative and positive control.</span><br/> |
− | + | ||
− | <span style="font-size: | + | |
</caption> | </caption> | ||
<tbody> | <tbody> | ||
<tr class="firstRow"> | <tr class="firstRow"> | ||
− | <td style=" | + | <td align="center" valign="middle" colspan="1" rowspan="1" style="border: 1px solid rgb(221, 221, 221);" width="277"></td> |
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" colspan="3" rowspan="1" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">Negative Control</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" colspan="3" rowspan="1" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">Positive Control<br/></span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | </tr> |
− | <span style="font-size: | + | <tr> |
+ | <td style="word-break: break-all; border: 1px solid rgb(221, 221, 221);" align="center" valign="middle" width="160"></td> | ||
+ | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="160"> | ||
+ | <span style="font-size: 18px;">D1</span> | ||
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="187"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">D2</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="187"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">D3</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="187"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">E1</span> |
+ | </td> | ||
+ | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="277"> | ||
+ | <span style="font-size: 18px;">E2</span> | ||
+ | </td> | ||
+ | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="160"> | ||
+ | <span style="font-size: 18px;">E3</span> | ||
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="160"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">Replicate1</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(235, 241, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(235, 241, 221);" width="160"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">802</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(235, 241, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(235, 241, 221);" width="187"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">785</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(235, 241, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(235, 241, 221);" width="187"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">777</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(242, 220, 219);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(242, 220, 219);" width="187"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">10593</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(242, 220, 219);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(242, 220, 219);" width="277"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">10104</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(242, 220, 219);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(242, 220, 219);" width="160"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">10021</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="160"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">Replicate2</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(235, 241, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(235, 241, 221);" width="160"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">700</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(235, 241, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(235, 241, 221);" width="187"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">769</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(235, 241, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(235, 241, 221);" width="187"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">752</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(242, 220, 219);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(242, 220, 219);" width="187"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">10758</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(242, 220, 219);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(242, 220, 219);" width="277"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">10161</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(242, 220, 219);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(242, 220, 219);" width="160"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">10136</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="160"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">Replicate3</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(235, 241, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(235, 241, 221);" width="160"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">1026</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(235, 241, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(235, 241, 221);" width="187"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">1022</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(235, 241, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(235, 241, 221);" width="187"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">1051</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(242, 220, 219);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(242, 220, 219);" width="187"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">10990</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(242, 220, 219);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(242, 220, 219);" width="277"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">10357</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(242, 220, 219);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); background-color: rgb(242, 220, 219);" width="160"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">10357</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="160"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">F</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="160"> |
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="187"> |
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="187"> |
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="187"> |
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="277"> |
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="160"> |
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="160"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">G</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="160"> |
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="187"> |
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="187"> |
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="187"> |
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="undefined"> |
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="undefined"> |
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
</tr> | </tr> | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
− | < | + | </br> |
− | + | <p class="main_p"> | |
− | + | D1-D3 wells=Negative control device (only promoter). </br> | |
− | + | E1-E3 wells=Positive control device (I20270 in PSB1C3 backbone);</br> | |
− | <p class=" | + | F wells=LB media plus antibiotic (chloramphenicol only).</br> |
− | + | G wells=non-inoculate cultures</br> | |
− | + | Three technical replicates (horizontal), three biological replicates(vertical) for A, B, C, and D.</br> | |
− | + | 485 ex/520 em; Units are arbitrary. | |
− | + | ||
− | + | ||
− | + | ||
</p> | </p> | ||
+ | </br> | ||
<p class="main_p">Data was processed as follows:</br> | <p class="main_p">Data was processed as follows:</br> | ||
1. Background absorbance was removed by subtracting the value of F wells.</br> | 1. Background absorbance was removed by subtracting the value of F wells.</br> | ||
Line 699: | Line 779: | ||
4. Evaluate the variability of chosen analysis technique.</br> | 4. Evaluate the variability of chosen analysis technique.</br> | ||
</p> | </p> | ||
− | + | <hr> | |
− | <table | + | <table class="mytable" height="160px" width="800px" align="center"> |
− | <caption style="border-color: rgb(221, 221, 221);"> | + | <caption style="border-color: rgb(221, 221, 221); text-align: center; vertical-align: middle;"> |
− | + | <span style="font-size: 18px;">Table 3a. Fluorescence of 3 different constructs expressing GFP.</span> | |
</caption> | </caption> | ||
<tbody> | <tbody> | ||
<tr class="firstRow"> | <tr class="firstRow"> | ||
− | <td style=" | + | <td style="border: 1px solid rgb(221, 221, 221); word-break: break-all;" align="center" valign="middle"></td> |
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">A1</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">A2</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">A3</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">B1</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">B2</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">B3</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">C1</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">C2</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">C3</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td align="center" valign="middle" style="word-break: break-all; border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="word-break: break-all; border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">Mean</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="word-break: break-all; border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="word-break: break-all; border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">56766</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="word-break: break-all; border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="word-break: break-all; border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">54909</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="word-break: break-all; border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="word-break: break-all; border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">54179</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="word-break: break-all; border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="word-break: break-all; border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">36610</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="word-break: break-all; border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="word-break: break-all; border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">34140</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">34139</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">1028</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">1138</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">1134</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">S. D.</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">1102.99</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">883.59</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">1179.83</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">1893.04</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">2064.66</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">1973.19</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">226.44</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">217.90</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">216.08</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">C. V.</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">1.94</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">1.61</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">2.18</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">5.17</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">6.05</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">5.78</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">22.02</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">19.15</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">19.05</span> |
</td> | </td> | ||
</tr> | </tr> | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
− | + | <table class="mytable" width="600px" height="200px" align="center" data-sort="sortDisabled"> | |
− | <table width="600px" height=" | + | <caption style="border-color: rgb(221, 221, 221); text-align: center; vertical-align: middle;"> |
− | <caption style="border-color: rgb(221, 221, 221);"> | + | <span style="font-size: 18px;">Table 3b. Positive and negative controls of fluorescence</span> |
− | <span style="font-size: | + | |
</caption> | </caption> | ||
<tbody> | <tbody> | ||
<tr class="firstRow"> | <tr class="firstRow"> | ||
− | <td style=" | + | <td align="center" valign="middle" colspan="1" rowspan="1" style="border: 1px solid rgb(221, 221, 221);" width="166"></td> |
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" colspan="3" rowspan="1" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">Negative Control</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221); | + | <td align="center" valign="middle" colspan="3" rowspan="1" style="border: 1px solid rgb(221, 221, 221);"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">Positive Control<br/></span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | </tr> |
− | <span style="font-size: | + | <tr> |
+ | <td style="word-break: break-all; border: 1px solid rgb(221, 221, 221);" align="center" valign="middle" width="192"></td> | ||
+ | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="192"> | ||
+ | <span style="font-size: 18px;">D1</span> | ||
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="192"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">D2</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="192"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">D3</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="192"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">E1</span> |
+ | </td> | ||
+ | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="166"> | ||
+ | <span style="font-size: 18px;">E2</span> | ||
+ | </td> | ||
+ | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="192"> | ||
+ | <span style="font-size: 18px;">E3</span> | ||
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="192"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">Mean</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="192"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">751</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="192"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">859</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="192"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">860</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="192"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">10676</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="166"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">10207</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="192"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">10171</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="192"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">S. D.</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="192"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">166.76</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="192"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">141.68</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="192"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">165.88</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="192"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">199.44</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="166"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">132.71</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="192"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">170.76</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="192"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">C. V.</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="192"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">22.21</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="192"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">16.49</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="192"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">19.29</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="192"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">1.87</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="undefined"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">1.30</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width=" | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="undefined"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">1.68</span> |
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td align="left" valign="top" colspan="7" rowspan="1" style="border-left-color: rgb(221, 221, 221); border-left-width: 1px; border-top-color: rgb(221, 221, 221); border-top-width: 1px;"> | ||
+ | <span style="font-family: Helvetica, Tahoma, Arial, 'Hiragino Sans GB', 'Hiragino Sans GB W3', 'Microsoft YaHei', STXihei, STHeiti, Heiti, SimSun, sans-serif; font-size: 16px; line-height: 27px; background-color: rgb(255, 255, 255);">S. D.-standard deviation. C.V-coefficient of variation.</span> | ||
</td> | </td> | ||
</tr> | </tr> | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
− | + | <h1 id="Provenance" class="main_h1">Ⅳ. Provenance</h1> | |
− | <h1 id="Provenance" class="main_h1"> | + | |
<p class="main_p">Q: Who did the actual work to acquire these measurements?</br> | <p class="main_p">Q: Who did the actual work to acquire these measurements?</br> | ||
A: Beibei Fang.</br> | A: Beibei Fang.</br> | ||
Line 940: | Line 1,032: | ||
</br> | </br> | ||
Q: On what dates were the protocols run and the measurements taken?</br> | Q: On what dates were the protocols run and the measurements taken?</br> | ||
− | A: Required devices were constructed by 30<sup>th</sup> | + | A: Required devices were constructed by 30<sup>th</sup> July 2015. All samples were measured on 12<sup>th</sup> August 2015. |
</p> | </p> | ||
− | <h1 id="Appendix" class="main_h1"> | + | <h1 id="Appendix" class="main_h1">Ⅴ. Appendix: Raw Data (Ex485 /Em520)</h1> |
− | + | <p class="replicate">Technical replicate 1</p> | |
− | + | <table class="mytable" align="center" width="600px" height="360px" interlaced="disabled"> | |
− | <p class=" | + | |
− | <table align="center" width="600px" height="360px" interlaced="disabled"> | + | |
<tbody> | <tbody> | ||
<tr class="firstRow"> | <tr class="firstRow"> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"></td> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"></td> |
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">1</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">2</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">3</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">4</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">5</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">A</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="text-align: -webkit-center;">/</span> | + | <span style="text-align: -webkit-center; font-size: 18px;">/</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="text-align: -webkit-center;">/</span> | + | <span style="text-align: -webkit-center; font-size: 18px;">/</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="text-align: -webkit-center;">/</span> | + | <span style="text-align: -webkit-center; font-size: 18px;">/</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">mean</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">B</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="text-align: -webkit-center;">/</span> | + | <span style="text-align: -webkit-center; font-size: 18px;">/</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="text-align: -webkit-center;">/</span> | + | <span style="text-align: -webkit-center; font-size: 18px;">/</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="text-align: -webkit-center;">/</span> | + | <span style="text-align: -webkit-center; font-size: 18px;">/</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="text-align: -webkit-center;">/</span> | + | <span style="text-align: -webkit-center; font-size: 18px;">/</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="text-align: -webkit-center;">/</span> | + | <span style="text-align: -webkit-center; font-size: 18px;">/</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">C</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">57850</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">55682</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">56413</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">56648</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="text-align: -webkit-center;">/</span> | + | <span style="text-align: -webkit-center; font-size: 18px;">/</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">D</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">36054</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">37165</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">33475</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">35565</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="text-align: -webkit-center;">/</span> | + | <span style="text-align: -webkit-center; font-size: 18px;">/</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">E</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">958</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">1098</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">1401</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">1152</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="text-align: -webkit-center;">/</span> | + | <span style="text-align: -webkit-center; font-size: 18px;">/</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">F</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">802</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">700</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">1026</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">843</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="text-align: -webkit-center;">/</span> | + | <span style="text-align: -webkit-center; font-size: 18px;">/</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">G</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">10593</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">10758</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">10990</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">10780</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="text-align: -webkit-center;">/</span> | + | <span style="text-align: -webkit-center; font-size: 18px;">/</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">H</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">6937</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">6892</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">7183</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="font-size: | + | <span style="font-size: 18px;">7004</span> |
</td> | </td> | ||
− | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | + | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78" class="selectTdClass"> |
− | <span style="text-align: -webkit-center;">/</span> | + | <span style="text-align: -webkit-center; font-size: 18px;">/</span> |
</td> | </td> | ||
</tr> | </tr> | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
− | <p class=" | + | <p class="replicate"> |
− | <table align="center" width="600px" height="360px"> | + | Technical replicate 2 |
+ | </p> | ||
+ | <table class="mytable" align="center" width="600px" height="360px"> | ||
<tbody> | <tbody> | ||
<tr class="firstRow"> | <tr class="firstRow"> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"></td> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"></td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">1</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">2</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">3</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">4</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">5</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">A</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="text-align: -webkit-center;">/</span> | + | <span style="text-align: -webkit-center; font-size: 18px;">/</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="text-align: -webkit-center;">/</span> | + | <span style="text-align: -webkit-center; font-size: 18px;">/</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="text-align: -webkit-center;">/</span> | + | <span style="text-align: -webkit-center; font-size: 18px;">/</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">mean</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">B</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="text-align: -webkit-center;">/</span> | + | <span style="text-align: -webkit-center; font-size: 18px;">/</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="text-align: -webkit-center;">/</span> | + | <span style="text-align: -webkit-center; font-size: 18px;">/</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="text-align: -webkit-center;">/</span> | + | <span style="text-align: -webkit-center; font-size: 18px;">/</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="text-align: -webkit-center;">/</span> | + | <span style="text-align: -webkit-center; font-size: 18px;">/</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">C</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">55881</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">54154</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">54693</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">55018</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="text-align: -webkit-center;">/</span> | + | <span style="text-align: -webkit-center; font-size: 18px;">/</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">D</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">34549</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">35969</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">31901</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">35259</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="text-align: -webkit-center;">/</span> | + | <span style="text-align: -webkit-center; font-size: 18px;">/</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">E</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">960</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">1073</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">1381</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">1017</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="text-align: -webkit-center;">/</span> | + | <span style="text-align: -webkit-center; font-size: 18px;">/</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">F</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">785</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">769</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">1022</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">777</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="text-align: -webkit-center;">/</span> | + | <span style="text-align: -webkit-center; font-size: 18px;">/</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">G</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">10104</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">10161</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">10357</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">10133</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="text-align: -webkit-center;">/</span> | + | <span style="text-align: -webkit-center; font-size: 18px;">/</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">H</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">6802</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">6783</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">7072</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">6793</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="text-align: -webkit-center;">/</span> | + | <span style="text-align: -webkit-center; font-size: 18px;">/</span> |
</td> | </td> | ||
</tr> | </tr> | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
− | <p class=" | + | <p class="replicate"> |
− | <table width="600px" height="360px" align="center"> | + | Technical replicate 3 |
+ | </p> | ||
+ | <table class="mytable" width="600px" height="360px" align="center"> | ||
<tbody> | <tbody> | ||
<tr class="firstRow"> | <tr class="firstRow"> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"></td> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"></td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">1</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">2</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">3</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">4</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">5</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">A</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">mean</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">B</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | / | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">C</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">55501</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">53233</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">53803</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">54179</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">D</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">34799</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">35697</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">31920</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">34139</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">E</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">957</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">1071</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">1375</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">1134</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">F</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">777</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">752</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">1051</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">860</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">G</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">10021</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">10136</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">10357</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">10171</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">H</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">6783</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">6762</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">6989</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">6845</span> |
</td> | </td> | ||
<td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | <td align="center" valign="middle" style="border: 1px solid rgb(221, 221, 221);" width="78"> | ||
− | <span style="font-size: | + | <span style="font-size: 18px;">/</span> |
</td> | </td> | ||
</tr> | </tr> | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
− | <h1 id="Reference" class="main_h1"> | + | <h1 id="Reference" class="main_h1">Ⅵ. Reference</h1> |
<p class="main_p">1. Anderson,C., Berkley iGEM Team., (2006). Anderson Promoter Collection. Registry of Standard Biological Parts.</br> | <p class="main_p">1. Anderson,C., Berkley iGEM Team., (2006). Anderson Promoter Collection. Registry of Standard Biological Parts.</br> | ||
2. <a href="http://parts.igem.org/Promoters/Catalog/Anderson">http://parts.igem.org/Promoters/Catalog/Anderson</a> [Accessed 2014.09.19]</br> | 2. <a href="http://parts.igem.org/Promoters/Catalog/Anderson">http://parts.igem.org/Promoters/Catalog/Anderson</a> [Accessed 2014.09.19]</br> | ||
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Latest revision as of 03:48, 19 September 2015
INTERLAB
Ⅰ. Introduction
The goal of the Interlab study is to obtain fluorescence data for three specific genetic devices expressing GFP from iGEM teams around the world. This year, three devices were cloned by ourselves, and one positive control and one negative control were provided by the registry. Using a plate reader, fluorescence measurements were obtained in arbitrary units. The results show increased fluorescence in the stronger promoter expected. After the experiment, three required devices were created: J23101+I13504 in the pSB3C3 backbone. J23106+I13504 in the pSB1C3 backbone. J23117+I13504 in the pSB1C3 backbone. Devices constructed in pSB1C3 are high copy number plasmids, hence a strong fluorescence can be obviously observed by naked eyes. While for device J23117 + I13504, the promoter strength is the weakest, we could barely observe any fluorescence under natural light.
Figure 1. GFP generator with different promoters. 1. J23101+I13504 in DH5α; 2. J23106+I13504 in DH5α; 3. J23117+I13504 in DH5α.
Figure 2. Centrifugation of bacteria. 1. J23101+I13504; 2. J23106+I13504; 3. J23117+I13504; 4. Positive control (I20270); 5. Negative control (R0040 in pSB1C3)
1. Digestion Verification
Four devices (J23101+I13504/J23106+I13504/J23117+I13504/I20270) are double digested at EcoR I and Pst I restriction sites to verify the target parts. The restriction map is shown as Figure 3. As expected, the second bands are supposed to be aroud 1000bp, which is consistent with what we saw on the map.
Figure 3. Restriction map of digestion verification. 2000plus DNA marker. 1. Double digestion of J23101+I13504 (constructed plasmid) 2. Double digestion of J23106+I13504 (constructed plasmid) 3. Double digestion of J23117+I13504 (constructed plasmid) 4. Double digestion of I20270 in pSB1C3 backbone (provided in registry)
2. DNA sequencing
All created parts are verified by DNA sequencing, analyzed by DNAMAN software, as following:
J23101+I13504.txt | J23106+I13504.txt | J23117+I13504.txt |
Ⅱ. Protocol
1. Transform constructed plasmids into DH5α competent cells, grown in incubator for 12 hrs at 37℃. 2. Add 5 mL LB medium with antibiotic (Chloramphenicol 35 μg/mL) into test tubes, choose monoclonal cells from the petri dish. 3. Set up 3 biological replicates of each device. Cultures were grown in test tubes for 16 hours at 37℃, shaking at 200 rpm. 4. Obtain initial OD 600 measurement of overnight cultures. Then dilute each sample to an OD of 0.5 (margin of error: 5%). 5. Set instrument to measure GFP. 6. Measurements of absorbance and fluorescence: (1) OD 600 Device: Spectrophotometer Wavelengths: 600 nm absorption. (2) Fluorescence Device: FLUOstar omega microplate reader, 96-well plates. Wavelengths: 485 nm excitation, 520 nm emission.
Ⅲ. Measurements
Absorbance at 600 nm was measured for each of the three cultures, alongside controls for media and contamination background. The dilution required for each sample has been calculated. Table 1 presents the re-measurement of samples on OD 600, and all OD 600 are within 5% of 0.5 (0.475-0.525).
J23101+I13504 | J23106+I13504 | J23117+I13504 | Negative | Positive | LB+Antibiotic | |
A | B | C | D | E | F | |
1 | 0.509 | 0.500 | 0.502 | 0.484 | 0.479 | 0.089 |
2 | 0.499 | 0.500 | 0.507 | 0.519 | 0.497 | 0.074 |
3 | 0.505 | 0.497 | 0.503 | 0.483 | 0.509 | 0.080 |
G | / | / | / | / | / | / |
D wells=negative control (R0040 in pSB1C3 backbone); E wells=positive control (I20270 in pSB1C3 backbone); F wells=LB media plus antibiotic (chloramphenicol only); G wells=Non-inoculate culture;
Table 2 presents the Fluorescence we measured, using three biological replicates of each device, alongside controls for LB media plus antibiotic and background value.
J23101+I13504 | J23106+I13504 |
J23117+I3504 |
|||||||
A1 | A2 | A3 | B1 | B2 | B3 | C1 | C2 | C3 | |
Replicate1 | 57850 | 55881 | 55501 | 36054 | 34549 | 34799 | 958 | 960 | 957 |
Replicate2 | 55682 | 54154 | 53233 | 37165 | 35969 | 35697 | 1098 | 1073 | 1071 |
Replicate3 | 56413 | 54693 | 53803 | 33475 | 31901 | 31920 | 1401 | 1381 | 1375 |
F | 6937 | 6892 | 7183 | / | / | / | / | / | / |
G | 114 | 108 | 105 | / | / | / | / | / | / |
Negative Control |
Positive Control |
|||||
D1 | D2 | D3 | E1 | E2 | E3 | |
Replicate1 | 802 | 785 | 777 | 10593 | 10104 | 10021 |
Replicate2 | 700 | 769 | 752 | 10758 | 10161 | 10136 |
Replicate3 | 1026 | 1022 | 1051 | 10990 | 10357 | 10357 |
F | / | / | / | / | / | / |
G | / | / | / | / | / | / |
D1-D3 wells=Negative control device (only promoter). E1-E3 wells=Positive control device (I20270 in PSB1C3 backbone); F wells=LB media plus antibiotic (chloramphenicol only). G wells=non-inoculate cultures Three technical replicates (horizontal), three biological replicates(vertical) for A, B, C, and D. 485 ex/520 em; Units are arbitrary.
Data was processed as follows: 1. Background absorbance was removed by subtracting the value of F wells. 2. Background fluorescence was controlled for by subtracting the value of F wells. 3. Calculate the mean value and standard deviation of the 3 biological replicates and 3 technical replicates. 4. Evaluate the variability of chosen analysis technique.
A1 | A2 | A3 | B1 | B2 | B3 | C1 | C2 | C3 | |
Mean | 56766 | 54909 | 54179 | 36610 | 34140 | 34139 | 1028 | 1138 | 1134 |
S. D. | 1102.99 | 883.59 | 1179.83 | 1893.04 | 2064.66 | 1973.19 | 226.44 | 217.90 | 216.08 |
C. V. | 1.94 | 1.61 | 2.18 | 5.17 | 6.05 | 5.78 | 22.02 | 19.15 | 19.05 |
Negative Control |
Positive Control |
|||||
D1 | D2 | D3 | E1 | E2 | E3 | |
Mean | 751 | 859 | 860 | 10676 | 10207 | 10171 |
S. D. | 166.76 | 141.68 | 165.88 | 199.44 | 132.71 | 170.76 |
C. V. | 22.21 | 16.49 | 19.29 | 1.87 | 1.30 | 1.68 |
S. D.-standard deviation. C.V-coefficient of variation. |
Ⅳ. Provenance
Q: Who did the actual work to acquire these measurements? A: Beibei Fang. Q: What other people should be credited for these measurements? A: Shouqiang Hong, Na Li. Q: On what dates were the protocols run and the measurements taken? A: Required devices were constructed by 30th July 2015. All samples were measured on 12th August 2015.
Ⅴ. Appendix: Raw Data (Ex485 /Em520)
Technical replicate 1
1 | 2 | 3 | 4 | 5 | |
A | / | / | / | mean | / |
B | / | / | / | / | / |
C | 57850 | 55682 | 56413 | 56648 | / |
D | 36054 | 37165 | 33475 | 35565 | / |
E | 958 | 1098 | 1401 | 1152 | / |
F | 802 | 700 | 1026 | 843 | / |
G | 10593 | 10758 | 10990 | 10780 | / |
H | 6937 | 6892 | 7183 | 7004 | / |
Technical replicate 2
1 | 2 | 3 | 4 | 5 | |
A | / | / | / | mean | / |
B | / | / | / | / | / |
C | 55881 | 54154 | 54693 | 55018 | / |
D | 34549 | 35969 | 31901 | 35259 | / |
E | 960 | 1073 | 1381 | 1017 | / |
F | 785 | 769 | 1022 | 777 | / |
G | 10104 | 10161 | 10357 | 10133 | / |
H | 6802 | 6783 | 7072 | 6793 | / |
Technical replicate 3
1 | 2 | 3 | 4 | 5 | |
A | / | / | / | mean | / |
B | / | / | / | / | / |
C | 55501 | 53233 | 53803 | 54179 | / |
D | 34799 | 35697 | 31920 | 34139 | / |
E | 957 | 1071 | 1375 | 1134 | / |
F | 777 | 752 | 1051 | 860 | / |
G | 10021 | 10136 | 10357 | 10171 | / |
H | 6783 | 6762 | 6989 | 6845 | / |
Ⅵ. Reference
1. Anderson,C., Berkley iGEM Team., (2006). Anderson Promoter Collection. Registry of Standard Biological Parts. 2. http://parts.igem.org/Promoters/Catalog/Anderson [Accessed 2014.09.19] 3. https://2014.igem.org/Team:XMU-China/Project_Interlab 4. https://2014.igem.org/Team:Imperial/InterLab_Study
CONTACT US
Email: igemxmu@gmail.com
Website: 2015.igem.org/Team:Amoy
Address: Xiamen University, No. 422, Siming South Road, Xiamen, Fujian, P.R.China 361005