Difference between revisions of "Team:Yale/parts"

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       <p> Our collection of submitted biobricks consists of:</p>
 
       <p> Our collection of submitted biobricks consists of:</p>
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        <li>Mussel foot protein (MFP) 1-5-1 sequence [combination of Mytilus galloprovincialis Foot Protein 5 (Mgfp-5) and Mytilus li Edulis Foot Protein 1 (Mefp-1)].</li>
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       <h2 id="partfull">New Biobricks</h2>
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        <li>MFP with superfolder Green Fluorescence Protein (sfGFP).</li>
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       <h3>Promoter-Citrine-T7 Terminator Constructs</h3>
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        <li>MFP with our anti-microbial peptide, LL-37.</li>
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        <li>Entire construct of our anti-microbial adhesive peptide: 2XStrep_Flagtag--LL-37--Mussel Foot Protein--sfGFP.   </li>
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       <h2 id="partfull">Full Molecule</h2>
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       <h3>BBa_K1396000</h3>
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           <h4>Testing Promoters</h4>
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           <h4>BBa_K1856000</h4>
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           <p>The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein-linked to superfolder GFP for localization. The mussel foot protein will anneal to surfaces as a wet glue and the antimicrobial domain is designed to interact with microbial membranes and interfere with membrane stability. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine supressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence supressing LL-37 antimicrobial action.</p>
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           <p>BacA-citrine construct. Leaky expression observed in E. coli. Successfully transformed into rhizobium. Sequencing confirmed</p>
 
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         <div class="small-7 columns readable"><img src="https://static.igem.org/mediawiki/2014/b/b7/Full_Construct.jpg"></div>
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         <div class="small-7 columns readable"><img src="https://2015.igem.org/File:Expression_Level_for_bacA-citrine_(pKT230-Lic).jpg"></div>
 
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Revision as of 03:51, 19 September 2015


<!DOCTYPE html> Yale iGem 2015: Project Parts

Developing a Framework for the Genetic Manipulation of Non-Model and Environmentally Significant Microbes

Overview

Methods

Results

Modeling

Parts

Parts List

These are our legos.

New BiobricksImproved Biobricks

Our collection of submitted biobricks consists of:

New Biobricks

Promoter-Citrine-T7 Terminator Constructs

BBa_K1856000

BacA-citrine construct. Leaky expression observed in E. coli. Successfully transformed into rhizobium. Sequencing confirmed

Part 1: LL-37-MFP:

Based on BBa_K1396000

Feeding Fish

The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein. The mussel foot protein will anneal to surfaces as a wet glue and the antimicrobial domain is designed to interact with microbial membranes and interfere with membrane stability. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine suppressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence suppressing LL-37 antimicrobial action. This is an improvement on the Utah State biobrick BBa_K1162006 which consists of only the LL-37 peptide.tion. This is an improvement on the Utah State biobrick BBa_K1162006 which consists of only the LL-37 peptide.

Yale iGem 2015

Main Campus:

Yale Department of Molecular, Cellular & Developmental Biology

Attn: Farren Isaacs/iGEM

219 Prospect St

PO Box 208103

New Haven, CT 06520

Tel. +1(203) 432-3783

E-mail: igem.yale@gmail.com

© Yale iGEM 2015