Difference between revisions of "Team:Yale/parts"

 
(5 intermediate revisions by 2 users not shown)
Line 26: Line 26:
 
           </ul>
 
           </ul>
 
         </li>
 
         </li>
−
        <li><a href="https://2015.igem.org/Team:Yale/notebook" alt="Notebook">Notebook</a></li>
+
         <li class="submenu"><a href="notebook">Notebook</a>
−
         <li class="submenu"><a href="collaborations">Collaborations</a>
+
 
           <ul>
 
           <ul>
−
             <li><a href="https://2015.igem.org/Team:Yale/collaborations#guidebook" alt="Handbook">Handbook</a></li>
+
             <li><a href="https://2015.igem.org/Team:Yale/notebook" alt="Weekly">Weekly</a></li>
−
             <li><a href="https://2015.igem.org/Team:Yale/collaborations#protocat" alt="Protocat">Protocat</a></li>
+
             <li><a href="https://static.igem.org/mediawiki/2015/f/fb/Yale_iGEM_Project_Summary_2015.pdf" alt="PDF Summary">PDF Summary</a></li>
 
           </ul>
 
           </ul>
 
         </li>
 
         </li>
 +
        <li class="submenu"><a href="collaborations">Collaborations</a></li>
 
         <li class="submenu"><a href="practices">Human Practices</a>
 
         <li class="submenu"><a href="practices">Human Practices</a>
 
           <ul>
 
           <ul>
Line 42: Line 42:
 
         <li class="submenu"><a href="team">Team</a>
 
         <li class="submenu"><a href="team">Team</a>
 
           <ul>
 
           <ul>
−
             <li><a href="https://2015.igem.org/Team:Yale/team#people" alt="People">People</a></li>
+
             <li><a href="https://2015.igem.org/Team:Yale/team" alt="People">People</a></li>
−
             <li><a href="https://2015.igem.org/Team:Yale/team#acknowledgements" alt="Acknowledgements">Acknowledgements</a></li>
+
             <li><a href="https://2015.igem.org/Team:Yale/Attributions" alt="Acknowledgements">Attributions</a></li>
 
           </ul>
 
           </ul>
 
         </li>
 
         </li>
Line 70: Line 70:
 
       <h2 id="overview">Parts List
 
       <h2 id="overview">Parts List
 
         <h3>These are our legos.</h3>
 
         <h3>These are our legos.</h3>
−
         <div class="page__button"><a href="#partfull" class="custom__button">Full Molecule</a><a href="#part1" class="custom__button">Part One</a><a href="#part2" class="custom__button">Part Two</a>
+
         <div class="page__button"><a href="#partfull" class="custom__button">New Biobricks</a><a href="#part1" class="custom__button">Improved Biobricks</a>
 
         </div>
 
         </div>
 
       </h2>
 
       </h2>
Line 76: Line 76:
 
     <section class="content__section">
 
     <section class="content__section">
 
       <p> Our collection of submitted biobricks consists of:</p>
 
       <p> Our collection of submitted biobricks consists of:</p>
−
      <ul>
+
    
−
        <li>Mussel foot protein (MFP) 1-5-1 sequence [combination of Mytilus galloprovincialis Foot Protein 5 (Mgfp-5) and Mytilus li Edulis Foot Protein 1 (Mefp-1)].</li>
+
       <h2 id="partfull">New Biobricks</h2>
−
        <li>MFP with superfolder Green Fluorescence Protein (sfGFP).</li>
+
       <h3>Promoter-Citrine-T7 Terminator Constructs</h3>
−
        <li>MFP with our anti-microbial peptide, LL-37.</li>
+
−
        <li>Entire construct of our anti-microbial adhesive peptide: 2XStrep_Flagtag--LL-37--Mussel Foot Protein--sfGFP.   </li>
+
−
      </ul>
+
−
    </section>
+
−
    <section class="content__section">
+
−
       <h2 id="partfull">Full Molecule</h2>
+
−
       <h3>BBa_K1396000</h3>
+
 
       <div class="row content__dark part__row">
 
       <div class="row content__dark part__row">
 
         <div class="small-5 columns readable">
 
         <div class="small-5 columns readable">
−
           <h4>Testing Promoters</h4>
+
           <h4>BBa_K1856000</h4>
−
           <p>The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein-linked to superfolder GFP for localization. The mussel foot protein will anneal to surfaces as a wet glue and the antimicrobial domain is designed to interact with microbial membranes and interfere with membrane stability. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine supressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence supressing LL-37 antimicrobial action.</p>
+
           <p>BacA-citrine construct. Leaky expression observed in E. coli. Successfully transformed into rhizobium. Sequencing confirmed</p>
 
         </div>
 
         </div>
−
         <div class="small-7 columns readable"><img src="https://static.igem.org/mediawiki/2014/b/b7/Full_Construct.jpg"></div>
+
         <div class="small-7 columns readable"><img src="https://static.igem.org/mediawiki/2015/a/ac/Expression_Level_for_bacA-citrine_%28pKT230-Lic%29.jpg"></div>
 
       </div>
 
       </div>
 
     </section>
 
     </section>
Line 104: Line 97:
 
         <div class="small-7 columns readable"><img src="https://static.igem.org/mediawiki/2014/f/f4/LL37_FP151.jpg">
 
         <div class="small-7 columns readable"><img src="https://static.igem.org/mediawiki/2014/f/f4/LL37_FP151.jpg">
 
         </div>
 
         </div>
−
      </div>
 
−
    </section>
 
−
    <section class="content__section">
 
−
      <h2 id="part2">Part 2: MFP-sfGFP: </h2>
 
−
      <h3>Based on BBa_K1396002</h3>
 
−
      <div class="row content__dark part__row">
 
−
        <div class="small-5 columns readable">
 
−
          <h4>Harvesting Oysters</h4>
 
−
          <p>The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein-linked to superfolder GFP for localization. The mussel foot protein will anneal to surfaces as a wet glue and superfolder GFP will allow for florescence imaging and localization. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine supressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence supressing LL-37 antimicrobial action.</p>
 
−
        </div>
 
−
        <div class="small-7 columns readable"><img src="https://static.igem.org/mediawiki/2014/5/5b/FP151_GFP.jpg" ,data-width="565" data-height="525" class="img"></div>
 
 
       </div>
 
       </div>
 
     </section>
 
     </section>

Latest revision as of 03:54, 19 September 2015


<!DOCTYPE html> Yale iGem 2015: Project Parts

Developing a Framework for the Genetic Manipulation of Non-Model and Environmentally Significant Microbes

Overview

Methods

Results

Modeling

Parts

Parts List

These are our legos.

New BiobricksImproved Biobricks

Our collection of submitted biobricks consists of:

New Biobricks

Promoter-Citrine-T7 Terminator Constructs

BBa_K1856000

BacA-citrine construct. Leaky expression observed in E. coli. Successfully transformed into rhizobium. Sequencing confirmed

Part 1: LL-37-MFP:

Based on BBa_K1396000

Feeding Fish

The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein. The mussel foot protein will anneal to surfaces as a wet glue and the antimicrobial domain is designed to interact with microbial membranes and interfere with membrane stability. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine suppressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence suppressing LL-37 antimicrobial action. This is an improvement on the Utah State biobrick BBa_K1162006 which consists of only the LL-37 peptide.tion. This is an improvement on the Utah State biobrick BBa_K1162006 which consists of only the LL-37 peptide.

Yale iGem 2015

Main Campus:

Yale Department of Molecular, Cellular & Developmental Biology

Attn: Farren Isaacs/iGEM

219 Prospect St

PO Box 208103

New Haven, CT 06520

Tel. +1(203) 432-3783

E-mail: igem.yale@gmail.com

© Yale iGEM 2015