Difference between revisions of "Team:BostonU"

 
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<h2> Welcome to iGEM 2015! </h2>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<h4>Before you start: </h4>
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<p> Please read the following pages:</p>
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<ul>
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<li>  <a href="https://2015.igem.org/Requirements">Requirements page </a> </li>
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<li> <a href="https://2015.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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<h4> Styling your wiki </h4>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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<h4> Editing your wiki </h4>
 
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
 
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<p> <a href="https://2015.igem.org/wiki/index.php?title=Team:BostonU&action=edit"> Click here to edit this page! </a></p>
 
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<p>See tips on how to edit your wiki on the <a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation</a> page.</p>
 
  
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<h2 style="font-color:#000000; margin-left:50px;">Developing conditionally dimerizable split protein systems for genetic logic and genome editing applications </h2>
  
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<h4>Templates </h4>
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<p style="padding-bottom:50px;">The field of synthetic biology seeks to engineer desirable cellular functionalities by developing molecular technologies that enable precise genetic manipulation. A promising solution is to reliably control proteins that naturally execute genetic modifications. Current strategies to regulate activity of such proteins primarily rely on modulating protein expression level through transcriptional control; however, these methods are susceptible to slow response and leaky expression. In contrast, strategies that exploit post-translational regulation of activity, such as conditional dimerization of split protein halves, have been demonstrated to bypass these limitations. Here, we compare the relative efficiency of previously characterized dimerization domains in regulating activities of three important genetic manipulation proteins - integrases and recombination directionality factors for genetic logic applications, and saCas9 for in vivo genome editing applications. We also establish guidelines to rationally identify promising protein split sites. Our characterization of these systems in mammalian cells ultimately paves way for important biomedical applications.</p>
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<p> This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the  
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<a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation </a> page.</p>  
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<td><center><a href="https://2015.igem.org/Team:BostonU/Temporal_Control"><img style="height:85%; width:85%;" src="https://static.igem.org/mediawiki/2015/thumb/9/99/Active_and_inactive_protein.png/800px-Active_and_inactive_protein.png" /></a><center></td>
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<td><center><a href="https://2015.igem.org/Team:BostonU/App_1/Motivation"><img style="height:85%; width:85%; padding-left:30px; padding-right:30px;" src="https://static.igem.org/mediawiki/2015/thumb/8/86/Home_page_integrase_RDF.png/562px-Home_page_integrase_RDF.png" /></a></center></td>
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<td><center><a href="https://2015.igem.org/Team:BostonU/App_2/Motivation"><img style="height:60%; width:60%;" src="https://static.igem.org/mediawiki/2015/thumb/1/1c/Crispr_cas9_labeled.png/800px-Crispr_cas9_labeled.png" /></a></center></td>
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</tr>
  
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<h4>Tips</h4>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<td><h3 align="center" style="padding-bottom:50px;">Controlling Protein Activity</h3></td>
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<ul>
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<td><h3 align="center" style="padding-bottom:50px;">Controlling Integrases and RDFs</h3></td>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<td><h3 align="center" style="padding-bottom:50px;">Controlling saCas9</h3></td>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2015.igem.org/Calendar_of_Events">iGEM 2015 calendar</a> </li>
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<li>Have lots of fun! </li>
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</ul>  
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</tr>
  
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<h4>Inspiration</h4>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<td><center><a href="https://2015.igem.org/Team:BostonU/Mammalian_synbio/Significance"><img style="height:50%; width:50%;" src="https://static.igem.org/mediawiki/2015/thumb/5/59/Mammalian_syn_bio_home_page.png/618px-Mammalian_syn_bio_home_page.png" /></a></center></td>
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<ul>
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<td><center><a align="center" href="https://2015.igem.org/Team:BostonU/Education/Building_with_Biology"><img style="height:95%; width:95%; padding-left:30px; padding-right:30px;" src="https://static.igem.org/mediawiki/2015/c/c5/Building_w_biology.jpg" /></a></center></td>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<td><center><a href="https://2015.igem.org/Team:BostonU/Attributions"><img style="height:200px; width:200px;" src="https://static.igem.org/mediawiki/2015/3/39/Thank_you_attributions.png"/></a></center></td>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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</div>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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</ul>
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<h4> Uploading pictures and files </h4>
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<p> You can upload your pictures and files to the iGEM 2015 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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<td><h3 align="center">Mammalian Synthetic Biology</h3></td>
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When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<td><h3 align="center">Education and Outreach</h3></td>
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<td><h3 align="center">Attributions</h3></td>
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</tr>
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</table>
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<br><br><br><br><br><br><br><br>
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<h4 style="font-size:25px; text-align:center;">iGEM Jamboree 2015 Results</h4>
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<ol style="font-size:23px;text-align:center">Medal Received: Gold.<br>Nominated for Best Foundational Advance Project</li>
  
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<a href="https://2015.igem.org/Special:Upload">CLICK HERE TO UPLOAD FILES</a>
 
  
  
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Latest revision as of 20:30, 3 October 2015

Home

Developing conditionally dimerizable split protein systems for genetic logic and genome editing applications

The field of synthetic biology seeks to engineer desirable cellular functionalities by developing molecular technologies that enable precise genetic manipulation. A promising solution is to reliably control proteins that naturally execute genetic modifications. Current strategies to regulate activity of such proteins primarily rely on modulating protein expression level through transcriptional control; however, these methods are susceptible to slow response and leaky expression. In contrast, strategies that exploit post-translational regulation of activity, such as conditional dimerization of split protein halves, have been demonstrated to bypass these limitations. Here, we compare the relative efficiency of previously characterized dimerization domains in regulating activities of three important genetic manipulation proteins - integrases and recombination directionality factors for genetic logic applications, and saCas9 for in vivo genome editing applications. We also establish guidelines to rationally identify promising protein split sites. Our characterization of these systems in mammalian cells ultimately paves way for important biomedical applications.

Controlling Protein Activity

Controlling Integrases and RDFs

Controlling saCas9

Mammalian Synthetic Biology

Education and Outreach

Attributions








iGEM Jamboree 2015 Results

    Medal Received: Gold.
    Nominated for Best Foundational Advance Project