Difference between revisions of "Team:KU Leuven/Research/Basic Part"

 
(24 intermediate revisions by 4 users not shown)
Line 44: Line 44:
 
}
 
}
  
 +
.summaryimg{
 +
opacity:0.8;
 +
}
 +
 +
td img {
 +
margin:5%;
 +
}
 
</style>
 
</style>
  
Line 50: Line 57:
 
<div class="summaryheader">
 
<div class="summaryheader">
 
   <div class="summaryimg">
 
   <div class="summaryimg">
−
   <img src="https://static.igem.org/mediawiki/2015/b/b7/KU_Leuven_Researchbanner.jpg" width="100%">
+
   <img src="https://static.igem.org/mediawiki/2015/5/5b/KU_Leuven_Banner_Geel.jpg" width="100%">
 
   <div class="head">
 
   <div class="head">
−
     <h2> Basics Parts </h2>
+
     <h2> Basic Parts </h2>
 
   </div>
 
   </div>
 
  </div>
 
  </div>
Line 67: Line 74:
 
     <th>Construct</th>
 
     <th>Construct</th>
 
     <th>Length</th>
 
     <th>Length</th>
−
     <th>Description</th>
+
     <th>Type</th>
 
</tr>
 
</tr>
 
<tr class="lightrow">
 
<tr class="lightrow">
−
     <td><a href="http://parts.igem.org/Part:BBa_K1709002" target='_blank_'>BBa_K1709002</a></td>  
+
     <td><a href="http://parts.igem.org/Part:BBa_K1709002" target='_blank_'>BBa_K1709002</a><img src="https://static.igem.org/mediawiki/2015/e/e1/KU_Leuven_Heart.png" width="4%"></td>  
 
     <td>CheZ-GFP</td>
 
     <td>CheZ-GFP</td>
 
     <td>1401 bp</td>
 
     <td>1401 bp</td>
−
     <td><a href="#">text</a></td>
+
     <td>Coding</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
Line 79: Line 86:
 
     <td>LuxI-His</td>
 
     <td>LuxI-His</td>
 
     <td>609 bp</td>
 
     <td>609 bp</td>
−
     <td><a href="#">text</a></td>
+
     <td>Coding</td>
 
</tr>
 
</tr>
 
<tr class="lightrow">
 
<tr class="lightrow">
Line 85: Line 92:
 
     <td>LuxR-E</td>
 
     <td>LuxR-E</td>
 
     <td>835 bp</td>
 
     <td>835 bp</td>
−
     <td><a href="#">text</a></td>
+
     <td>Coding</td>
 
</tr>
 
</tr>
−
<tr class="lightrow">
 
 
     <td><a href="http://parts.igem.org/Part:BBa_K1709005" target='_blank_'>BBa_K1709005</a></td>
 
     <td><a href="http://parts.igem.org/Part:BBa_K1709005" target='_blank_'>BBa_K1709005</a></td>
 
     <td>Ag43-YFP</td>
 
     <td>Ag43-YFP</td>
 
     <td>3843 bp</td>
 
     <td>3843 bp</td>
−
     <td><a href="#">text</a></td>
+
     <td>Coding</td>
 
</tr>
 
</tr>
 
</table>
 
</table>
Line 100: Line 106:
 
<br/>
 
<br/>
  
−
<div class="center">
 
−
 
−
    <div class="togglebar">
 
−
        <div class="toggleone">
 
−
            <h2>CheZ-GFP</h2>
 
−
        </div>
 
−
      <div id="toggleone">
 
−
            <br/>
 
−
 
−
 
−
<div class="togglebar">
 
−
<div class="toggletwo">
 
−
<h2>LuxI-His</h2>
 
−
</div>
 
−
<div id="toggletwo" >
 
−
<p><b>Theory</b><br/>
 
−
 
−
</div>
 
−
</div>
 
 
</br>
 
</br>
−
<div class="togglebar">
 
−
<div class="togglethree">
 
−
<h2>Motility Test Assay</h2>
 
−
</div>
 
−
<div id="togglethree" >
 
−
<p><b>Protocol</b></p>
 
−
<dl>
 
−
<dd>1. Prepare selective media (LB with 0.25% agar (2,5 g/L) in Petri dishes (85 mm dia).</dd>
 
−
<dd>2. Apply 1.5 µL of the diluted cell suspensions from mid-log-phase cultures (~2×105 cells/µL (OD=0.5)) to the center of the plates, and let dry in air for 15 min. </dd>
 
−
<dd>3. Incubate at 37 °C for 10 h. </dd>
 
−
</dl>
 
−
 
−
</div>
 
−
</div>
 
−
</br>
 
−
</div>
 
−
 
−
</div>
 
−
</div>
 
  
 
<div class="whiterow"></div>
 
<div class="whiterow"></div>
Line 478: Line 446:
 
   </div>
 
   </div>
 
  <div id="saillart">
 
  <div id="saillart">
−
       <a href="http://www.glasatelier-saillart.be/"><img src="https://static.igem.org/mediawiki/2015/c/ce/KU_Leuven_Sponsor_Saillard.png" alt="Glasatelier Saillart" width="95%"></a>
+
       <a href="http://www.glasatelier-saillart.be/English/english.html"><img src="https://static.igem.org/mediawiki/2015/c/ce/KU_Leuven_Sponsor_Saillard.png" alt="Glasatelier Saillart" width="95%"></a>
 
   </div>
 
   </div>
 
<div id="kuleuven">
 
<div id="kuleuven">
Line 506: Line 474:
 
   </div>
 
   </div>
 
</div>
 
</div>
 +
<div class="logonormal2">
 
<div id="vwr">
 
<div id="vwr">
 
       <a href="https://be.vwr.com/store/?&_requestid=866148&_DARGS=/store/cms/be.vwr.com/nl_BE/header_20159241139103.jsp.1_AF&_dynSessConf=4047468000326453053&targetURL=/store/%3F%26_requestid%3D866148&lastLanguage=en&/vwr/userprofiling/EditPersonalInfoFormHandler.updateLocale=&_D%3AcurrentLanguage=+&currentLanguage=en&_D%3AlastLanguage=+&_D%3A/vwr/userprofiling/EditPersonalInfoFormHandler.updateLocale=+"><img src="https://static.igem.org/mediawiki/2015/8/8d/KU_Leuven_Logo_VWR_transparant_.png" alt="VWR" width="95%"></a>
 
       <a href="https://be.vwr.com/store/?&_requestid=866148&_DARGS=/store/cms/be.vwr.com/nl_BE/header_20159241139103.jsp.1_AF&_dynSessConf=4047468000326453053&targetURL=/store/%3F%26_requestid%3D866148&lastLanguage=en&/vwr/userprofiling/EditPersonalInfoFormHandler.updateLocale=&_D%3AcurrentLanguage=+&currentLanguage=en&_D%3AlastLanguage=+&_D%3A/vwr/userprofiling/EditPersonalInfoFormHandler.updateLocale=+"><img src="https://static.igem.org/mediawiki/2015/8/8d/KU_Leuven_Logo_VWR_transparant_.png" alt="VWR" width="95%"></a>
 
   </div>
 
   </div>
 +
<div class = "whiterow"></div>
 +
<div id="lgc">
 +
<a href="http://www.lgcgroup.com/our-science/genomics-solutions/#.Vfx9V9yLTIU">
 +
                <img src="https://static.igem.org/mediawiki/2015/e/e6/KU_Leuven_LOGO_LGC.png" alt="LGC Genomics" width="80%">
 +
</a>
 +
</div>
 +
</div>
 
  <div id="footerimg">
 
  <div id="footerimg">
 
   <img src="https://static.igem.org/mediawiki/2015/b/b9/KU_Leuven_Zebra_spots_wiki_footer_main.png" width="95%">
 
   <img src="https://static.igem.org/mediawiki/2015/b/b9/KU_Leuven_Zebra_spots_wiki_footer_main.png" width="95%">
 
   </div>
 
   </div>
 +
<div class="logonormal2">
 
  <div id="gimv">
 
  <div id="gimv">
 
       <a href="http://www.gimv.com/en"><img src="https://static.igem.org/mediawiki/2015/a/ac/KU_Leuven_Logo_Gimv_Transparant.png" alt="Gimv" width="95%"></a>
 
       <a href="http://www.gimv.com/en"><img src="https://static.igem.org/mediawiki/2015/a/ac/KU_Leuven_Logo_Gimv_Transparant.png" alt="Gimv" width="95%"></a>
 
   </div>
 
   </div>
 +
<div class = "whiterow"></div>
 +
<div id="sopach">
 +
      <a href="http://www.sopachem.com/"><img src="https://static.igem.org/mediawiki/2015/5/55/KU_Leuven_Sopachem.jpeg" alt="Sopachem" width="95%"></a>
 +
  </div>
 +
</div>
 
   <div id="machery">
 
   <div id="machery">
 
       <a href="http://www.filterservice.be/"><img src="https://static.igem.org/mediawiki/2015/4/41/KU_Leuven_Macherey_Nagel_logo_transparant.png" alt="Machery Nagel" width="95%"></a>
 
       <a href="http://www.filterservice.be/"><img src="https://static.igem.org/mediawiki/2015/4/41/KU_Leuven_Macherey_Nagel_logo_transparant.png" alt="Machery Nagel" width="95%"></a>

Latest revision as of 09:34, 20 October 2015

Logo

Basic Parts

On this page you can find all our basic parts needed for the system to work. To learn more about them, click the titles below!

Name Construct Length Type
BBa_K1709002 CheZ-GFP 1401 bp Coding
BBa_K1709000 LuxI-His 609 bp Coding
BBa_K1709004 LuxR-E 835 bp Coding
BBa_K1709005 Ag43-YFP 3843 bp Coding


Idea

A detailed description about the interaction between our two cells and the genetic circuit can be found here.

Methods

Here you can find the performed steps to create two different cell types and detailed quantification methods to determine interesting parameters.
Read more
Read more

Composite Parts

Our basic parts were combined with each other and with existing iGEM promotors, RBS and terminators.

Results

The results of our experiments will appear here.

Back

Go back to the Research page.

Read more
Read more
Read more
Idea

A detailed description about the interaction between our two cells and the genetic circuit can be found here.

Methods

Here you can find the performed steps to create two different cell types and detailed quantification methods to determine interesting parameters.

Composite Parts

Our basic parts were combined with each other and with existing iGEM promotors, RBS and terminators.

Results

Click here to discover our results

Back

Go back to the Research page.

Contact

Address: Celestijnenlaan 200G room 00.08 - 3001 Heverlee
Telephone: +32(0)16 32 73 19
Email: igem@chem.kuleuven.be