Difference between revisions of "Team:Uppsala"

 
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{{Template_All_Teams}}
+
{{Uppsala_NewTemplate}}
 
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+
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+
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+
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+
 
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+
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 +
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*/
 
  
 +
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 +
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 +
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/*Hide the sub-menus*/
+
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+
 
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+
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+
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+
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+
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+
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+
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+
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+
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+
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+
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+
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+
 
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+
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+
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+
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+
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+
 
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/* Change of link text in the bar*/
+
 
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+
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+
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+
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+
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+
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+
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+
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+
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+
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+
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+
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+
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+
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+
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+
 
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+
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+
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+
 
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+
 
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+
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+
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+
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+
 
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+
 
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+
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+
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 +
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 +
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</style>
 
</style>
 
 
</head>
 
</head>
 
<body>
 
<body>
  
<!--<nav class="fixed">
+
    <!--<img id="home_image" src="https://static.igem.org/mediawiki/2015/4/4d/Uppsala_NewFront.png" alt="iGEM Uppsala" style="width:100%;height:100%;">-->
<ul class="navbar">
+
  <li class="listelem"><a href="https://2015.igem.org/Team:Uppsala">Home</a></li>
+
  <li class="listelem"><a href="https://2015.igem.org/Team:Uppsala/Team">Team</a></li>
+
  <li class="listelem"><a href="https://2015.igem.org/Team:Uppsala/Project">Project
+
      <ul>
+
<a href="https://2015.igem.org/Team:Uppsala/Description"><li>Description</li></a>
+
<a href="https://2015.igem.org/Team:Uppsala/Experiments"><li>Experiments &amp; Protocols</li></a> 
+
<a href="https://2015.igem.org/Team:Uppsala/Results"><li>Results</li></a> 
+
<a href="https://2015.igem.org/Team:Uppsala/Design"><li>Design</li></a>
+
      </ul>
+
  </a></li>
+
  <li class="listelem"><a href="https://2015.igem.org/Team:Uppsala/Parts">Parts
+
      <ul>
+
<a href="https://2015.igem.org/Team:Uppsala/Parts"><li>Team Parts</li></a>
+
<a href="https://2015.igem.org/Team:Uppsala/Basic_Part"><li>Basic Parts</li></a> 
+
<a href="https://2015.igem.org/Team:Uppsala/Composite_Part"><li>Composite Parts</li></a>
+
<a href="https://2015.igem.org/Team:Uppsala/Part_Collection"><li>Part Collection</li></a> 
+
      </ul>
+
  </a></li>
+
  <li><a href="https://2015.igem.org/Team:Uppsala/Notebook">Notebook</a></li>
+
  <li><a href="https://2015.igem.org/Team:Uppsala/Attributions">Attributions</a></li>
+
  <li><a href="https://2015.igem.org/Team:Uppsala/Collaborations">Collaborations</a></li>
+
  <li><a href="https://2015.igem.org/Team:Uppsala/Practices">Human Practices</a></li>
+
  <li><a href="https://2015.igem.org/Team:Uppsala/Safety">Safety</a></li>
+
  <li><a href="https://2015.igem.org/Team:Uppsala/Modeling">Modeling</a></li>
+
  <li><a href="https://2015.igem.org/Team:Uppsala/Measurement">Measurement</a></li>
+
  <li><a href="https://2015.igem.org/Team:Uppsala/Software">Software</a></li>
+
  <li><a href="https://2015.igem.org/Team:Uppsala/Entrepreneurship">Entrepreneurship</a></li>
+
</ul>
+
</nav>-->
+
<div id="cssmenu"> <nav class="fixed">
+
<ul class="navbar">
+
  <li class="listelem"><a href="https://2015.igem.org/Team:Uppsala">Home</a></li>
+
  <li class="listelem"><a href="https://2015.igem.org/Team:Uppsala/Team">Team</a></li>
+
  <li class="listelem"><a href="https://2015.igem.org/Team:Uppsala/Project">Project
+
      <ul>
+
<a href="https://2015.igem.org/Team:Uppsala/Description"><li>Description</li></a>
+
<a href="https://2015.igem.org/Team:Uppsala/Experiments"><li>Experiments &amp; Protocols</li></a> 
+
<a href="https://2015.igem.org/Team:Uppsala/Results"><li>Results</li></a> 
+
<a href="https://2015.igem.org/Team:Uppsala/Design"><li>Design</li></a>
+
      </ul>
+
  </a></li>
+
  <li class="listelem"><a href="https://2015.igem.org/Team:Uppsala/Parts">Parts
+
      <ul>
+
<a href="https://2015.igem.org/Team:Uppsala/Parts"><li>Team Parts</li></a>
+
<a href="https://2015.igem.org/Team:Uppsala/Basic_Part"><li>Basic Parts</li></a> 
+
<a href="https://2015.igem.org/Team:Uppsala/Composite_Part"><li>Composite Parts</li></a>
+
<a href="https://2015.igem.org/Team:Uppsala/Part_Collection"><li>Part Collection</li></a> 
+
      </ul>
+
  </a></li>
+
  <li><a href="https://2015.igem.org/Team:Uppsala/Notebook">Notebook</a></li>
+
  <li><a href="https://2015.igem.org/Team:Uppsala/Attributions">Attributions</a></li>
+
  <li><a href="https://2015.igem.org/Team:Uppsala/Collaborations">Collaborations</a></li>
+
  <li><a href="https://2015.igem.org/Team:Uppsala/Practices">Human Practices</a></li>
+
  <li><a href="https://2015.igem.org/Team:Uppsala/Safety">Safety</a></li>
+
  <li><a href="https://2015.igem.org/Team:Uppsala/Modeling">Modeling</a></li>
+
  <li><a href="https://2015.igem.org/Team:Uppsala/Measurement">Measurement</a></li>
+
  <li><a href="https://2015.igem.org/Team:Uppsala/Software">Software</a></li>
+
  <li><a href="https://2015.igem.org/Team:Uppsala/Entrepreneurship">Entrepreneurship</a></li>
+
</ul>
+
</nav></div>  
+
  
<div>
+
<div class="flip-container">
<img id="home_image" src="https://static.igem.org/mediawiki/2015/8/8a/Uppsala_HomeHeader.png" alt="iGEM Uppsala" style="width:960px;height:611px;>
+
<div class="flipper" onclick="this.classList.toggle('flipped')">
 +
<div class="front">
 +
<img id="home_image" src="https://static.igem.org/mediawiki/2015/4/4d/Uppsala_NewFront.png" alt="iGEM Uppsala" style="width:100%;">
 +
</div>
 +
<div class="back">
 +
<img id="home_image" src="https://static.igem.org/mediawiki/2015/7/72/Uppsala_MansFront.png" alt="iGEM Uppsala" style="width:100%;">
 +
</div>
 +
</div>
 
</div>
 
</div>
  
     <h1 class="header">Home</h1>
+
     <div id="main">
 +
 
 +
   
 
     <h2 class="header">Abstract</h2>
 
     <h2 class="header">Abstract</h2>
 
     <hr>
 
     <hr>
     <p>
+
      
     Nature is a biologist’s working bench - and as such we like to keep it tidy. Polycyclic aromatic hydrocarbons (PAHs) are a major pollutant derived from human activity - coil burning, oil spills, gasification plant waste. It’s carcinogenic, insoluble in water and hard to degrade. Thankfully, we’re also engineers and we decided to help nature do it’s thing.
+
  <table>
    </p>
+
    <tr>
    <p>
+
    <td>
    We took a twoway approach for the project  first we’ll enhance E.coli with the ability to degrade PAHs by inserting a biobrick construct  containing laccase  a powerful enzyme that helps get rid of those pesky compounds. And second, we’ll develop a biosensor, so that bacteria don’t waste their time producing enzymes when there is nothing to break down.
+
      <p>
    </p>
+
     Polycyclic aromatic hydrocarbons (PAHs) are potent carcinogens produced by various activities ranging from grilling meat to coal gasification. Our project aims to degrade high molecular weight PAHs present in industrial waste from district heating plants. There is currently no sustainable way to handle this waste and therefore it is deposited in landfills. We want to solve this problem by  employing a bacterial based system.  
    <p>
+
    </p>
    PAHs are a major problem because they are stable and hydrophobic  meaning once an area is polluted, cleaning up is very hard  like trying to wash a greasy pan by dipping it in cold water! By using the help of bacteria, these contaminants can be removed from the environment (for example in wastewater treatment plants). Best of all  it will require minimum effort, because bacteria will degrade the compounds all by themselves, and with pleasure. Imagine eating doughnuts for homework!
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    <p>Our cells need to be able to detect the PAHs for direct degradation. However the high molecular weight PAHs do not readily pass through the cell membrane. We solved this obstacle by using the low weight PAH, naphthalene, as an indicator for the presence of heavier PAHs. The degradation of this small PAH inside the cell relieves repression of genes under the control of the NahR/Psal promoter system. This causes the expression of a series of enzymes for PAH degradation which are secreted outside the cell where they can oxidize and cleave the ring structures of the carcinogenic compounds. This in turn makes them available for downstream biodegradation. To increase the degradation efficiency, our system also produces rhamnolipids. These are biosurfactants that prevent the PAHs from aggregating, making them easier for the enzymes to access.  
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Latest revision as of 22:45, 20 November 2015

iGEM Uppsala
iGEM Uppsala

Abstract


Polycyclic aromatic hydrocarbons (PAHs) are potent carcinogens produced by various activities ranging from grilling meat to coal gasification. Our project aims to degrade high molecular weight PAHs present in industrial waste from district heating plants. There is currently no sustainable way to handle this waste and therefore it is deposited in landfills. We want to solve this problem by employing a bacterial based system.

Our cells need to be able to detect the PAHs for direct degradation. However the high molecular weight PAHs do not readily pass through the cell membrane. We solved this obstacle by using the low weight PAH, naphthalene, as an indicator for the presence of heavier PAHs. The degradation of this small PAH inside the cell relieves repression of genes under the control of the NahR/Psal promoter system. This causes the expression of a series of enzymes for PAH degradation which are secreted outside the cell where they can oxidize and cleave the ring structures of the carcinogenic compounds. This in turn makes them available for downstream biodegradation. To increase the degradation efficiency, our system also produces rhamnolipids. These are biosurfactants that prevent the PAHs from aggregating, making them easier for the enzymes to access.