Difference between revisions of "Team:Uppsala"

 
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    <!--<img id="home_image" src="https://static.igem.org/mediawiki/2015/4/4d/Uppsala_NewFront.png" alt="iGEM Uppsala" style="width:100%;height:100%;">-->
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<img id="home_image" src="https://static.igem.org/mediawiki/2015/4/4d/Uppsala_NewFront.png" alt="iGEM Uppsala" style="width:100%;">
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<div class="back">
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<img id="home_image" src="https://static.igem.org/mediawiki/2015/7/72/Uppsala_MansFront.png" alt="iGEM Uppsala" style="width:100%;">
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    <img id="home_image" src="https://static.igem.org/mediawiki/2015/0/08/Uppsala_NewFrontHeader.png" alt="iGEM Uppsala" style="width:1100px;height:517px;">
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−
     <h1 class="header">Home</h1>
+
      
 
     <h2 class="header">Abstract</h2>
 
     <h2 class="header">Abstract</h2>
 
     <hr>
 
     <hr>
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     <p>
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     Nature is a biologist’s working bench - and as such we like to keep it tidy. Polycyclic aromatic hydrocarbons (PAHs) are a major pollutant derived from human activity - coil burning, oil spills, gasification plant waste. It’s carcinogenic, insoluble in water and hard to degrade. Thankfully, we’re also engineers and we decided to help nature do it’s thing.
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  <table>
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    We took a two-way approach for the project  first we’ll enhance E.coli with the ability to degrade PAHs by inserting a biobrick construct  containing laccase  a powerful enzyme that helps get rid of those pesky compounds. And second, we’ll develop a biosensor, so that bacteria don’t waste their time producing enzymes when there is nothing to break down.
+
      <p>
−
    </p>
+
     Polycyclic aromatic hydrocarbons (PAHs) are potent carcinogens produced by various activities ranging from grilling meat to coal gasification. Our project aims to degrade high molecular weight PAHs present in industrial waste from district heating plants. There is currently no sustainable way to handle this waste and therefore it is deposited in landfills. We want to solve this problem by  employing a bacterial based system.  
−
    <p>
+
    </p>
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    PAHs are a major problem because they are stable and hydrophobic  meaning once an area is polluted, cleaning up is very hard  like trying to wash a greasy pan by dipping it in cold water! By using the help of bacteria, these contaminants can be removed from the environment (for example in wastewater treatment plants). Best of all  it will require minimum effort, because bacteria will degrade the compounds all by themselves, and with pleasure. Imagine eating doughnuts for homework!
+
    <p>Our cells need to be able to detect the PAHs for direct degradation. However the high molecular weight PAHs do not readily pass through the cell membrane. We solved this obstacle by using the low weight PAH, naphthalene, as an indicator for the presence of heavier PAHs. The degradation of this small PAH inside the cell relieves repression of genes under the control of the NahR/Psal promoter system. This causes the expression of a series of enzymes for PAH degradation which are secreted outside the cell where they can oxidize and cleave the ring structures of the carcinogenic compounds. This in turn makes them available for downstream biodegradation. To increase the degradation efficiency, our system also produces rhamnolipids. These are biosurfactants that prevent the PAHs from aggregating, making them easier for the enzymes to access.  
−
     </p>
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      </p>
 +
      </td>
 +
      <td>
 +
      <a href="https://2015.igem.org/Team:Uppsala/Results">
 +
        <img id="results_button" src="https://static.igem.org/mediawiki/2015/e/eb/Uppsala_ResultsButton.png" style="width:90px;">
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      </a>
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      </td>
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    </tr>
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     </table>
 
     <hr>
 
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                    <div id="med" >
 +
<a href="https://2015.igem.org/Team:Uppsala/Journey">
 +
                              <p class="description">Click here to read about our journey to gold!</p>
 +
      <img id="medal" src="https://static.igem.org/mediawiki/2015/f/f7/Uppsala_goldmedal.png">
 +
                              <!--<h2><span id="textbox">Nordic iGEM Conference</span></h2>-->
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</a>
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                </td>
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  <a href="#">
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  <a href="https://2015.igem.org/Team:Uppsala/Enzymes">
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<img class="button" src="" alt="">
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                                        <p class="description">Laccase and dioxygenase are enzymes that are central to our project. Click here to learn more about how we use them!</p>
−
                                         <h2><span id="textbox">Enzymatic Degradation</span></h2>
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<img class="button" src="https://static.igem.org/mediawiki/2015/9/95/Uppsala_Degradation.png" alt="Enyzmatic degradation" style="width:350px;height:583px">
 +
                                         <!--<h2><span id="textbox">Enzymatic Degradation</span></h2>-->
 
    </a>
 
    </a>
 
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<td class="buttons">
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<td >
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<div>
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<a href="#">
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<a href="https://2015.igem.org/Team:Uppsala/Naphthalene">
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<img src="" alt="">
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                              <p class="description">The degradation of naphthalene is what activates our whole construct. Click here to learn more about how this happens!</p>
−
                                <h2><span id="textbox">Biosurfactants</span></h2>
+
      <img src="https://static.igem.org/mediawiki/2015/9/92/Uppsala_NewNahButton.png" alt="Naphthalene pathway" style="width:350px;height:583px">
 +
                              <!--<h2><span id="textbox">Naphthalene Pathway</span></h2>-->
 
</a>
 
</a>
 
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</td>
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                <td >
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<a href="https://2015.igem.org/Team:Uppsala/Biosurfactants">
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      <img src="" alt="">
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                                <p class="description">We used rhamnolipids to increase our enzymes' accessibility to the PAH:s. Click here to find out more about how we incorporated them into our system!</p>
−
                              <h2><span id="textbox">Naphthalene Pathway</span></h2>
+
<img src="https://static.igem.org/mediawiki/2015/7/73/Uppsala_NewBiosurfButton.png" alt="Biosurfactants" style="width:350px;height:583px">
 +
                                <!--<h2><span id="textbox">Biosurfactants</span></h2>-->
 
</a>
 
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</tr>
 
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<a href="https://2015.igem.org/Team:Uppsala/Software">
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                               <h2><span id="textbox">Results</span></h2>
+
                              <p class="description">We needed a fluorometer to measure the efficiency of our system, but we quickly noticed that getting hold of one was not that easy. Therefore, we built our own!</p>
 +
      <img src="https://static.igem.org/mediawiki/2015/2/27/Uppsala_fluorometer.png" alt="NiC" style="width:1100px; height:263px">
 +
                               <!--<h2><span id="textbox">Nordic iGEM Conference</span></h2>-->
 
</a>
 
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                               <h2><span id="textbox">Nordic iGEM Conference</span></h2>
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                              <p class="description">We collaborated with the iGEM team from Stockholm to arrange a meetup of nordic teams in Uppsala. Click here to learn more about the Nordic iGEM Conference!</p>
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Latest revision as of 22:45, 20 November 2015

iGEM Uppsala
iGEM Uppsala

Abstract

Polycyclic aromatic hydrocarbons (PAHs) are potent carcinogens produced by various activities ranging from grilling meat to coal gasification. Our project aims to degrade high molecular weight PAHs present in industrial waste from district heating plants. There is currently no sustainable way to handle this waste and therefore it is deposited in landfills. We want to solve this problem by employing a bacterial based system.

Our cells need to be able to detect the PAHs for direct degradation. However the high molecular weight PAHs do not readily pass through the cell membrane. We solved this obstacle by using the low weight PAH, naphthalene, as an indicator for the presence of heavier PAHs. The degradation of this small PAH inside the cell relieves repression of genes under the control of the NahR/Psal promoter system. This causes the expression of a series of enzymes for PAH degradation which are secreted outside the cell where they can oxidize and cleave the ring structures of the carcinogenic compounds. This in turn makes them available for downstream biodegradation. To increase the degradation efficiency, our system also produces rhamnolipids. These are biosurfactants that prevent the PAHs from aggregating, making them easier for the enzymes to access.

Laccase and dioxygenase are enzymes that are central to our project. Click here to learn more about how we use them!

Enyzmatic degradation

The degradation of naphthalene is what activates our whole construct. Click here to learn more about how this happens!

Naphthalene pathway

We used rhamnolipids to increase our enzymes' accessibility to the PAH:s. Click here to find out more about how we incorporated them into our system!

Biosurfactants