Difference between revisions of "Team:Tokyo-NoKoGen"

 
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{{TeamTokyoNokogenheader}}
 
 
{{TeamTokyoNokogen}}
 
{{TeamTokyoNokogen}}
<html>
 
  
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<html lang="ja">
 
<style>
 
<style>
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 +
margin: 0; /* 余白の削除 */
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 +
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 +
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 +
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 +
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 +
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 +
        top:260px
 +
}
 +
 +
.overview {
 +
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 +
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 +
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 +
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 +
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 +
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 +
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.overview2{
 +
 width:500px;
 +
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 +
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 margin-left:15px;
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 +
 +
.team {
 +
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 +
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 +
   vertical-align: middle;
 +
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 +
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 +
 +
        .notebook  {
 +
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 +
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 +
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 +
      display: table-cell; 
 +
              display: inline-block; _display: inline;
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 +
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 +
width:900px;
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      top:340px;
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.white{
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background:#ffffff;
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 +
   
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 +
.facebook {
 +
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height:135px;
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      position:relative;
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margin-left:20px;
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background:#6b8bcd;
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display: inline-block; _display: inline;
 +
display: table-cell;    /* ④ */ 
 +
   vertical-align: middle;
 +
   position:absolute;
 +
left:15px;
 +
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 +
.twitter {
 +
width:200px;
 +
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margin-top:300px;
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margin-left:20px;
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background:#6b8bcd;
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display: table-cell;   
 +
      vertical-align: middle;  
 +
cursor:pointer;
 +
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 +
   
 +
 +
.project {
 +
width:650px;
 +
height:260px;
 +
margin-top:-290px;
 +
margin-right:0px;
 +
background: #79d480;
 +
float:right;
 +
cursor:pointer;
 +
}
 +
div#box3 /*3段目の箱*/ {
 +
width:900px;
 +
height:180px;
 +
margin-top:-19px;
 +
 +
        position:absolute;
 +
        top:350px
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 +
.Ache {
 +
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 +
.Human {
 +
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 +
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 +
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 +
.parts {
 +
width:210px;
 +
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 +
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 +
margin-left:10px;
 +
background:#7bd593;
 +
display: inline-block; _display: inline;
 +
float:left;
 +
cursor:pointer;
 +
 +
}
 +
      .safety {
 +
width:210px;
 +
height:150px;
 +
margin-top:-5px;
 +
margin-left:10px;
 +
background:#7593d3;
 +
        display: inline-block; _display: inline;
 +
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 +
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 +
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 +
 +
 
p {
 
p {
 
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     font-size:16px;
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     line-height:2;  
 
     line-height:2;  
 
   }
 
   }
h2 {
+
h2{
     font-size:20px;
+
     font-size:25px;
     font-family:Georgia;
+
     font-family:fantasy;
 +
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 +
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 +
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 +
    font-size:14px;
 +
    font-family:fantasy;
 
     text-decoration:none;
 
     text-decoration:none;
  }
+
    line-height: 120%;
 +
    }
 +
 
 +
h3{
 +
    font-size:14px;
 +
    font-family:fantasy;
 +
    text-decoration:none;
 +
    line-height: 120%;
 +
    }
  
 
div#main {
 
div#main {
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     background-color:#ffffff
 
     background-color:#ffffff
 
     text-align:center;
 
     text-align:center;
     top:12px;left:140px;
+
     top:12px;
 +
    left:140px;
 
     font-family:Georgia;
 
     font-family:Georgia;
 
     font-size:16px;
 
     font-size:16px;
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</style>
+
div#nav {
  <div id="main">
+
    width:100%;
 +
    margin:0 auto;
 +
    background-color:#E6FFE9
 +
    text-align:center;
 +
    position:fixed;
 +
    z-index: 100;
 +
    top:18px;
 +
        }
  
 +
div#nav td{
 +
width:300px;height:45px;
 +
        margin:0 auto;
 +
text-align:center;
 +
border:none;
 +
font-family:fantasy;
 +
        font-size:18px;
 +
}
  
<h2> Team Project Descriptions </h2>
+
#globalWrapper, #content {
<h4>-Background-</h4>
+
width: 100%;
<p> Do you know serious pest damages that is harmful to human house and buildings…?<br>
+
height: 100%;
<p>The criminal is one of the flagitious vermin….termites.</p>
+
border: 40px;
<p>Termites are insects that feed on wood and paper, and eat like pillars of houses or books. There are a lot of  pesticides developed to exterminate termites. However, many of the pesticides against termites is an organophosphorus agent which has an immediate effect to the individual, while has a negative effect on the human body.</p>
+
background-color: #E6FFE9;
<p>To solve this problem of pesticides, NoKogen aim to engineer new termites extermination system in iGEM project. </p>
+
margin: 0px;
 +
padding: 0px;
 +
font-size: 100%;
 +
}
 +
html, body, .wrapper {
 +
width: 100%;
 +
height: 100%;
 +
background-color: #E6FFE9;
  
<h4>-Object-</h4>
+
.link_box{
<p> We have two objectives to realize our project.<br>
+
    position:;
      ・Exterminate termite in slow-acting method on a long-term basis<br>
+
    width:30%;
      ・Create a new pesticide which is not harmful to man  </p>
+
    padding:5%;
 +
    border:solid 0px #CCC;
 +
    margin:0px auto 20px;
 +
}
 +
 +
.link_box a{
 +
    position:absolute;
 +
    top:0;
 +
    left:0;
 +
    width:100%;
 +
    height:100%;
 +
    text-indent:-999px;
 +
}
 +
</style>
 +
<div id="wrap">
  
<h4>-Method-</h4>
+
<div id="bannerContainer"/*バナー*/>
<p> To achieve these objective, we are engineering the E. coli which produce poison against termites and work in termite’s guts.</p>
+
<img src="https://static.igem.org/mediawiki/2015/8/82/Tokyo-NoKogen_banner.png" width="900" height="250">  
<p> In our project, we focused on the two properties of termites.<br>
+
</div>
First, we  became aware of that cockroaches and termites are the same order and both of them use trehalose as the primary energy source. <br>
+
  <div id="box1"/*見えない1段目のボックス*/> 
Actually, NoKoGen engineered E. coli which has ability to kill cockroaches. So we thought that we can apply part of the method of previous project to exterminate termites. <br>
+
In concrete method, we are planning to use 3,3-diketotrehalose. it is an inhibitor of trehalase which converts trehalose to glucose.
+
Cockroach and termites digested carbohydrates and metabolized to trehalose.
+
Trehalose stored in the blood and is taken up by cells additionally converts trehalose to glucose.
+
3,3-diketotrehalose is produced by the enzymatic reaction of glucose 3 dehydrogenase (G3DH).<br>
+
We aim to express the G3DH in E. coli and prevent the trehalase activity by competitive inhibition.
+
</p>
+
<p> Moreover, we construct OtsA and OtsB which convert glucose to trehalose.<br>
+
Many more trehalose is converted to 3,3-diketotrehalose which inhibits termite’s passway.</p>
+
  
<p>
+
  <div class="overview">
Second properties of termites is that termites carry and lick their eggs.<br>
+
           <div class="overview2">
Termites recognize their egg as spherical material has a diameter of 0.5 mm  and pheromone.
+
<div style="padding: 5px; border: none; background-color: #ffffff; margin: 10px; "> <h2>Introduction</h2>
Based on this feature of termites, we try to make the pseudo-eggs to use the habit.<br>
+
<img src="https://static.igem.org/mediawiki/2015/e/e6/NK-image1.jpeg" align=right width=150px; height=120px;>
We are planning to make pseudo-eggs with LB agar which is cut to the spherical and engineered E. coli in it.
+
<h3>What kind  image do you have when you hear the word “termite”??<br><br>
We will treat this material as pseudo egg and apply the Lysozyme as termite’s pheromone so that
+
Actually, this tiny insect has unimaginable voracity. <br>
termites recognize them as eggs and take them to termites colony.<br>
+
They cause great damage for human life, but there is no way to exterminate terites completely and safely. <br><br>
We expect that this pseudo-eggs are effective to exterminate termites.</p>
+
To solve this problem, <br>Tokyo-NoKoGen “ExTermite Coli”, a new pesticides for Termites !!<br>
<p>By these methods based on the feature of termites , we hope that we can achieve our objectives.</p>
+
</h3>
 +
 +
</div>         
 +
<a href="https://2015.igem.org/Team:Tokyo-NoKoGen/Overview" target="_blank" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor='#e7e7e7' ></a>
 +
           </div>
 +
     </div>
 +
    
 +
    
 +
  <div class="team">
 +
<div style="padding: 5px; border: none; background-color: #ffffff; margin: 10px;"> <h2>Team</h2>
 +
<img src="https://static.igem.org/mediawiki/2015/0/0f/NK-image23.jpeg"  width=50px; height=50px;>
 +
</div>
 +
  <a href="https://2015.igem.org/Team:Tokyo-NoKoGen/Team" target="_blank" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7 ></a>
 +
         </div>
 +
        
 +
              <div class="notebook">
 +
<div style="padding: 5px; border: none; background-color: #ffffff; margin: 10px;">
 +
  <h2>Protocol</h2>
 +
<img src="https://static.igem.org/mediawiki/2015/5/5e/NK-image11.jpeg" width=50px; height=50px;>
 +
<br>
 +
</div>
 +
 <a href="https://2015.igem.org/Team:Tokyo-NoKoGen/Notebook" target="_blank" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7 ></a>
 +
          </div>
  
<h4>-Biosafety-</h4>
 
<p>When we think about practical application of our termite extermination system, there are one big problem. <br>
 
It is that we can’t avoid spreading gene recombinant microorganisms to the natural world.
 
However, there are many restriction about application of gene recombinant microorganisms not to destroy the balance of nature.<br>
 
To solve this problem and protect biosafety standards, we are planning to introduce bacteriolysis system in our project. <br>
 
Our target, termites usually form colonies around dark and shadowy area, like under the floor.
 
Inversely, our termites extermination system has no need to work in some places which are exposed to direct sunlight. So we are looking for some systems which can induce bacteriolysis to our engineered E.coli under white light or UV light.</p>
 
  
<h4>-Human practice-</h4>
+
       
<p>  
+
<div id="box2"/*見えない2段目のボックス*/> 
Medical drugs, cosmetics, genetically modified foods, detergents, and also pesticides….our everyday life is supported by cutting-edge biotechnology, but few people realize what you really use.<br>
+
<div class="facebook">
Tokyo-Nokogen is has some plans to spread some tips and knowledges about bioengineering and biotechnology.<br>
+
      <p style="position: relative;">
One of our plan is educational activity. We have already had a small class to teach biotechnology familiar to the students in the high-school. Also we asked them what kind of device they wanted  to create. There were some interesting ideas for us.<br>
+
        <a href="https://www.facebook.com/tokyo.nokogen" target="_blank">
Also, we are planning to take a survey to the people at large in Japan and other countries. </p>
+
         <img src="https://static.igem.org/mediawiki/2015/c/cc/NK-image17.jpeg" style ="position:absolute; top:0px;left:20px;" width="150" height="100"><br/>
<p>We expect that our survey will be one of a chance for people to take an interest in biotechnology. </p>
+
        <span style="position: absolute; top: 0px; left: 5px; width: 10 px;">
 +
         </span>
 +
        </a>
 +
              </p>
 +
    </div>
 +
  <div class="twitter">
 +
<div style="border: none; background-color: #ffffff; margin: 10px;">  
 +
         <h2>Twitter</h2>
 +
        <p style="position: relative;">
 +
                      <a href="https://twitter.com/iGEM_Nokogen" target="_blank">
 +
                      <img src="https://static.igem.org/mediawiki/2015/8/80/NKtwitter.jpeg" style ="position:absolute;top:-70px;left:20px;" width="135" height="90">
 +
                      </a>
 +
                    </p>
 +
                </div>
 +
    </div>
 +
        
 +
    <div class="project">
 +
       <div style="padding: 0px; border: none; background-color: #ffffff; margin: 4px;">
 +
<h2>project</h2>
 +
  <img src="https://static.igem.org/mediawiki/2015/2/23/NK-image7.jpeg" align=right width=130px; height=80px;>
 +
 
 +
<h3>The exterminateon of termites by ExTermite Coli involves three steps.<br>
 +
We use “pseudo egg” to distribute the engineered E.coli.  Using this unique tool, the engineered E.coli carried into the termite’s body. <br>
 +
In the termite’s body, it  produces toxic material to inhibit the metabolic pathway of the termites and exterminate termites.
 +
After extermination of the termites, ExTermite Coli loses its function by light-induced lysis. <br>
 +
  On the project page, we explain about the details of our project, ExTermite Coli.</h3>
  
 +
                          <span style="position: absolute; top: 5px; left: 5px; width: 10px;"></span>      
 +
     </div>
 +
     <a href="https://2015.igem.org/Team:Tokyo-NoKoGen/Description" target="_blank"></a>
  
 +
     </div>
 +
   
 +
      <div id="box3"/*見えない3段目のボックス*/>  
 +
    <div class="Ache">
 +
         <div style="padding: 5px; border: none; background-color: #ffffff; margin: 10px;">
 +
         <h2>Achievement</h2>
 +
                      </div>
 +
          <a href="https://2015.igem.org/Team:Tokyo-NoKoGen/achievement" target="_blank"></a>
 +
</div>
 +
    
 +
    
 +
    <div class="Human">
 +
          <div style="padding: 5px; border: none; background-color: #ffffff; margin: 10px;">
 +
          <h2>Human practice</h2>
 +
           </div>
 +
           <a href="https://2015.igem.org/Team:Tokyo-NoKoGen/Practices" target="_blank"></a>
 +
    </div>
 +
    
 +
    <div class="parts">
 +
          <div style="padding: 5px; border: none; background-color: #ffffff; margin: 10px;">
 +
          <h2>Parts</h2>
 +
</div>
 +
           <a href="https://2015.igem.org/Team:Tokyo-NoKoGen/Parts" target="_blank"></a>
 +
    </div>
 +
    
 +
    
 +
     <div class="safety">
 +
          <div style="padding: 5px; border: none; background-color: #ffffff; margin: 10px;">
 +
          <h2>Safety</h2>
 +
</div>
 +
           <a href="https://2015.igem.org/Team:Tokyo-NoKoGen/Safety" target="_blank"></a>
 +
    </div>
 +
    
 +
     
 +
      </div>
 +
     
 +
     
 +
     
 +
</div>        
 +
</div>
 +
<script type="text/javascript">
 +
$(function(){
 +
    $(".overview").click(function(){
 +
        window.location=$(this).find("a").attr("href");
 +
        return false;
 +
    });
 +
});
  
<h4>==============Default Wiki Page ===============</h4>
+
$(function(){
<p> Please read the following pages:</p>
+
    $(".team").click(function(){
<ul>
+
        window.location=$(this).find("a").attr("href");
<li>  <a href="https://2015.igem.org/Requirements">Requirements page </a> </li>
+
        return false;
<li> <a href="https://2015.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
+
    });
</ul>
+
});
 +
$(function(){
 +
    $(".facebook").click(function(){
 +
        window.location=$(this).find("a").attr("href");
 +
        return false;
 +
    });
 +
});
 +
$(function(){
 +
    $(".twitter").click(function(){
 +
        window.location=$(this).find("a").attr("href");
 +
        return false;
 +
    });
 +
});
 +
$(function(){
 +
    $(".notebook").click(function(){
 +
        window.location=$(this).find("a").attr("href");
 +
        return false;
 +
    });
 +
});
 +
$(function(){
 +
    $(".project").click(function(){
 +
        window.location=$(this).find("a").attr("href");
 +
        return false;
 +
    });
 +
});
 +
$(function(){
 +
    $(".Ache").click(function(){
 +
        window.location=$(this).find("a").attr("href");
 +
        return false;
 +
    });
 +
});
 +
$(function(){
 +
    $(".Human").click(function(){
 +
        window.location=$(this).find("a").attr("href");
 +
        return false;
 +
    });
 +
});
 +
$(function(){
 +
    $(".parts").click(function(){
 +
        window.location=$(this).find("a").attr("href");
 +
        return false;
 +
    });
 +
});
 +
$(function(){
 +
    $(".safety").click(function(){
 +
        window.location=$(this).find("a").attr("href");
 +
        return false;
 +
    });
 +
});
 +
</script>
  
<div class="highlightBox">
 
<h4> Styling your wiki </h4>
 
<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
 
<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
 
</div>
 
  
<h4> Editing your wiki </h4>
+
<!--footer-->
<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>  
+
<style>
<p> <a href="https://2015.igem.org/wiki/index.php?title=Team:Tokyo-NoKoGen&action=edit"> Click here to edit this page! </a></p>
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div#wrapper2 {
<p>See tips on how to edit your wiki on the <a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation</a> page.</p>
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width:80%;
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text-align:center;
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position:relative;
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min-height:100%;
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margin-left:auto;
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margin-right:auto;
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}
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div#footer {
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    width:900px;
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    position:relative;
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    left:-12%;
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    bottom:-1200px;
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    background-color:#ffffff;
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    border-width: thick;
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border-color: #ADFF2F;
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border-style:inset;
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        }
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div#footer img {
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    height:50px;
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        }
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p {
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    font-size:16px;
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    font-family:Georgia;
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    line-height:2;
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  }
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<h4>Templates </h4>
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</style>
<p> This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the
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<a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation </a> page.</p>  
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<h4>Tips</h4>
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<body>
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<ul>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2015.igem.org/Calendar_of_Events">iGEM 2015 calendar</a> </li>
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<li>Have lots of fun! </li>
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</ul>  
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<div id="wrapper2">
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<div id="footer">
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        <a href="http://www.asante.co.jp"><img src="https://static.igem.org/mediawiki/2015/a/a8/NK-image25.jpeg"></a>
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<a href="http://www.idtdna.com/site"><img src="https://static.igem.org/mediawiki/2014/5/5e/Noko14_IDT.png"></a>
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<a href="http://www.ultizyme.jp/"><img src="https://static.igem.org/mediawiki/2014/c/ce/Noko14_Image91.jpg"></a><br>
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<a href="http://www.cosmobio.co.jp/index_e.asp"><img src="https://static.igem.org/mediawiki/2014/e/e5/Noko14_Logo_e.jpg"></a>
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<a href="http://www.mbl.co.jp/e/index.html"><img src="https://static.igem.org/mediawiki/2014/4/4f/Noko14_MBL.gif"></a>
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<a href="http://www.idtdna.com/site"><img src="https://static.igem.org/mediawiki/2014/a/ad/Noko14_li.png"></a>
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<a href="http://www.tuat.ac.jp/en/index.html"><img src="https://static.igem.org/mediawiki/2014/3/39/Noko14_tuat.png"></a>
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<a href="http://www.tuat.ac.jp/~tanpaku/"><img src="https://static.igem.org/mediawiki/2014/3/3c/Noko14_ike.jpg"></a>
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<a href="http://www.tuat.ac.jp/~kakusan/index.html"><img src="https://static.igem.org/mediawiki/2014/7/77/Noko14_ikeken.jpg"></a>
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</div>
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</div>
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</body>
  
<h4>Inspiration</h4>
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<!--footer-->
<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<ul>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
+
<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
+
<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
+
<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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</ul>
+
  
<h4> Uploading pictures and files </h4>
 
<p> You can upload your pictures and files to the iGEM 2015 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
 
When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
 
  
<a href="https://2015.igem.org/Special:Upload">CLICK HERE TO UPLOAD FILES</a>
 
 
 
 
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</div>
 
 
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Latest revision as of 18:32, 23 November 2015

  
    
       

Introduction

What kind image do you have when you hear the word “termite”??

Actually, this tiny insect has unimaginable voracity.
They cause great damage for human life, but there is no way to exterminate terites completely and safely.

To solve this problem,
Tokyo-NoKoGen “ExTermite Coli”, a new pesticides for Termites !!

               
    
            

Team

    
  

Protocol


    
               
    

project

The exterminateon of termites by ExTermite Coli involves three steps.
We use “pseudo egg” to distribute the engineered E.coli. Using this unique tool, the engineered E.coli carried into the termite’s body.
In the termite’s body, it produces toxic material to inhibit the metabolic pathway of the termites and exterminate termites. After extermination of the termites, ExTermite Coli loses its function by light-induced lysis.
On the project page, we explain about the details of our project, ExTermite Coli.

          
       
     
       
       

Achievement

                  
       
        
       
       

Human practice

         
          
     
       
       

Parts

          
        
       
       

Safety