Difference between revisions of "Team:Tec-Monterrey"

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<h2> Welcome to iGEM 2015! </h2>
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<h1>Our Project:</h1>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<p>Bacteria and yeast cells have been used for a long time in protein production, but their main problem is that they aren’t able to fold complex proteins correctly and to do some posttranslational modifications. Looking for a solution to this problem, we are developing a toolkit box for the use of Sf9 cells for protein production. Sf9 cells are derived from Spodoptera frugiperda, the Fall Armyworm Moth, and are currently used for the production of vaccines and antibodies. This project will introduce 3 promoters for the use in baculovirus transfection and 1 promoter for stable line generation, 2 secretion signal peptides, 2 reporters, and T2A, all of them to simplify the use of this insect cell platform. Additionally, we will produce the Epidermal Growth Factor Receptor. We will also contrast the protein production levels through baculovirus transfection and stable line generation; this will allow other teams to choose the protein production method that better suits their needs. To accomplish this, first of all, a standardization of the protocols needed for the Sf9 cells growth and transfection will be done. Then, the basic components for the construction of biological circuits will be characterized. During this step, two protein production methods are going to be used: baculovirus transfection and stable line generation. At last, the system will be validated with the production of a complex model protein with posttranslational modifications, Epidermal Growth Factor Receptor. By the inclusion of this platform, it is expected that its use in synthetic biology will be increased, due to its high similarities to mammalian cells at a lower cost. Even though we consider our project to be foundational, we have in mind the future use of the Sf9 cells as the primary organism in which biopharmaceuticals could be produced, as the maintenance costs could be reduced in comparison to mammalian cells.</p>
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<h4>Before you start: </h4>
 
<h4>Before you start: </h4>

Revision as of 03:15, 15 July 2015


iGEM MTY 2015

Our Project:

Bacteria and yeast cells have been used for a long time in protein production, but their main problem is that they aren’t able to fold complex proteins correctly and to do some posttranslational modifications. Looking for a solution to this problem, we are developing a toolkit box for the use of Sf9 cells for protein production. Sf9 cells are derived from Spodoptera frugiperda, the Fall Armyworm Moth, and are currently used for the production of vaccines and antibodies. This project will introduce 3 promoters for the use in baculovirus transfection and 1 promoter for stable line generation, 2 secretion signal peptides, 2 reporters, and T2A, all of them to simplify the use of this insect cell platform. Additionally, we will produce the Epidermal Growth Factor Receptor. We will also contrast the protein production levels through baculovirus transfection and stable line generation; this will allow other teams to choose the protein production method that better suits their needs. To accomplish this, first of all, a standardization of the protocols needed for the Sf9 cells growth and transfection will be done. Then, the basic components for the construction of biological circuits will be characterized. During this step, two protein production methods are going to be used: baculovirus transfection and stable line generation. At last, the system will be validated with the production of a complex model protein with posttranslational modifications, Epidermal Growth Factor Receptor. By the inclusion of this platform, it is expected that its use in synthetic biology will be increased, due to its high similarities to mammalian cells at a lower cost. Even though we consider our project to be foundational, we have in mind the future use of the Sf9 cells as the primary organism in which biopharmaceuticals could be produced, as the maintenance costs could be reduced in comparison to mammalian cells.

Before you start:

Please read the following pages:

Styling your wiki

You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.

While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.

Editing your wiki

On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!

Click here to edit this page!

See tips on how to edit your wiki on the Template Documentation page.

Templates

This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the Template Documentation page.

Tips

This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:

  • State your accomplishments! Tell people what you have achieved from the start.
  • Be clear about what you are doing and how you plan to do this.
  • You have a global audience! Consider the different backgrounds that your users come from.
  • Make sure information is easy to find; nothing should be more than 3 clicks away.
  • Avoid using very small fonts and low contrast colors; information should be easy to read.
  • Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2015 calendar
  • Have lots of fun!

Inspiration

You can also view other team wikis for inspiration! Here are some examples:

Uploading pictures and files

You can upload your pictures and files to the iGEM 2015 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name.
When you upload, set the "Destination Filename" to Team:YourOfficialTeamName/NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)

CLICK HERE TO UPLOAD FILES