Difference between revisions of "Team:Kent/Notebook"

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<h1 align="center">Notebook</h1>
 
<h1 align="center">Notebook</h1>
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<div>
 
<div>
 
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.cont .hmain{background-color:#0babe7 ;width: 220px;font-size:16px;float: left;border-top:#fff thin solid;border-bottom:#fff thin solid;}   
 
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.cont a {text-decoration: none; /* padding-left: 10px;*/  display: block;  display: inline-block; width: 220px;padding-top: 7px;padding-bottom: 7px;}   
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.cont a {text-decoration: none; /* padding-left: 10px;*/  display: block;  display: inline-block; width: 220px;padding-top: 7px;padding-bottom: 7px; whitespace; nowrap}   
 
.cont .hmain a{color:#fff;  }
 
.cont .hmain a{color:#fff;  }
 
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                     <li>   
 
                     <li>   
 
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.table1{width:220px;}
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.table1{width:100px; colspan:2;}
 
.table1 td{color:#d2d2d2;background-color: #fff; margin:1px;width:27px;height:27px;text-align:center;}
 
.table1 td{color:#d2d2d2;background-color: #fff; margin:1px;width:27px;height:27px;text-align:center;}
 
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.table1 #alt{color:#fff;background-color: #4ccbfa; border:1px solid #fff;width:27px;height:27px;text-align:center;}
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.table1 th{font-size:14px;text-align:left;background-color:#000000;color:#fff; border:1px solid #fff;width:27px;height:27px;text-align:center;}
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.table1 th{font-size:14px;text-align:left;background-color:#fff;color:#000000; border:1px solid #fff;width:27px;height:27px;text-align:center; overflow: hidden;}
 
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<tr>
 
<tr>
−
<td>&nbsp;</td>
+
<th>&nbsp;</th>
−
<td>&nbsp;</td>
+
<th>1</th>
−
<td>&nbsp;</td>
+
<th>2</th>
−
<td>&nbsp;</td>
+
<th>3</th>
−
<td>&nbsp;</td>
+
<th>4</th>
−
<td>&nbsp;</td>
+
<th>5</th>
−
<td>1</td>
+
<th>6</th>
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
−
<td>2</td>
+
<th>7</th>
−
<td>3</td>
+
<th>8</th>
−
<td>4</td>
+
<th>9</th>
−
<td>5</td>
+
<th>10</th>
−
<td>6</td>
+
<th>11</th>
−
<td>7</td>
+
<th>12</th>
−
<td>8</td>
+
<th>13</th>
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
−
<td>9</td>
+
<th>14</th>
−
<td>10</td>
+
<th>15</th>
−
<td>11</td>
+
<th>16</th>
−
<td>12</td>
+
<th>17</th>
−
<td>13</td>
+
<th>18</th>
−
<td>14</td>
+
<th>19</th>
−
<td>15</td>
+
<th>20</th>
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
−
<td>16</td>
+
<th>21</td>
−
<td>17</td>
+
<th><a href="#day1">22</a></th>
−
<td>18</td>
+
<th><a href="#day2">23</a></th>
−
<td>19</td>
+
<th><a href="#day3">24</a></th>
−
<td>20</td>
+
<th><a href="#day4">25</a></th>
−
<td>21</td>
+
<th><a href="#day5">26</a></th>
−
<td><a href="#day1">22</a></td>
+
<th>27</th>
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
−
<td><a href="#day2">23</a></td>
+
<th>28</th>
−
<td><a href="#day3">24</a></td>
+
<th><a href="#day6">29</a></th>
−
<td><a href="#day4">25</a></td>
+
<th><a href="#day7">30</a></th>
−
<td><a href="#day5">26</a></td>
+
<th>&nbsp;</th>
−
<td>27</td>
+
<th>&nbsp;</th>
−
<td>28</td>
+
<th>&nbsp;</th>
−
<td><a href="#day6">29</a></td>
+
<th>&nbsp;</th>
 
</tr>
 
</tr>
−
 
−
<tr>
 
−
<td>30</td>
 
−
<td>&nbsp;</td>
 
−
<td>&nbsp;</td>
 
−
<td>&nbsp;</td>
 
−
<td>&nbsp;</td>
 
−
<td>&nbsp;</td>
 
−
<td>&nbsp;</td>
 
−
</tr>
 
−
 
  
 
</table>
 
</table>
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<tr>
 
<tr>
−
<td>&nbsp;</td>
+
<th>&nbsp;</th>
−
<td>1</td>
+
<th>&nbsp;</th>
−
<td>2</td>
+
<th>&nbsp;</th>
−
<td>3</td>
+
<th><a href="#day8">1</a></th>
−
<td>4</td>
+
<th><a href="#day9">2</a></th>
−
<td>5</td>
+
<th><a href="#day10">3</a></th>
−
<td>6</td>
+
<th>4</th>
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
−
<td>7</td>
+
<th>5</th>
−
<td>8</td>
+
<th><a href="#day11">6</a></th>
−
<td>9</td>
+
<th><a href="#day12">7</a></th>
−
<td>10</td>
+
<th><a href="#day13">8</a></th>
−
<td>11</td>
+
<th><a href="#day14">9</a></th>
−
<td>12</td>
+
<th><a href="#day15>10</a></th>
−
<td>13</td>
+
<th>11</th>
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
−
<td>14</td>
+
<th>12</th>
−
<td>15</td>
+
<th><a href="#day16">13</a></th>
−
<td>16</td>
+
<th><a href="#day 17">14</a></th>
−
<td>17</td>
+
<th><a href="#day18">15</a></th>
−
<td>18</td>
+
<th><a href="#day19">16</a></th>
−
<td>19</td>
+
<th>17</th>
−
<td>20</td>
+
<th>18</th>
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
−
<td>21</td>
+
<th>19</th>
−
<td>22</td>
+
<th>20</th>
−
<td>23</td>
+
<th>21</th>
−
<td>24</td>
+
<th>22</th>
−
<td>25</td>
+
<th>23</th>
−
<td>26</td>
+
<th>24</th>
−
<td>27</td>
+
<th>25</th>
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
−
<td>28</td>
+
<th>26</th>
−
<td>29</td>
+
<th>27</th>
−
<td>30</td>
+
<th>28</th>
−
<td>31</td>
+
<th>29</th>
−
<td>&nbsp;</td>
+
<th>30</th>
−
<td>&nbsp;</td>
+
<th>31</th>
−
<td>&nbsp;</td>
+
<th>&nbsp;</th>
 
</tr>
 
</tr>
  
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             </li>  
 
             </li>  
 
             <li class="hmain">  
 
             <li class="hmain">  
−
                 <a href="https://2015.igem.org/Team:Kent/Notebook">August,2015</a>
+
                 <a href="https://2015.igem.org/Team:Kent/Notebook">August 2015</a>
 
                 <ul>  
 
                 <ul>  
 
                     <li>
 
                     <li>
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<tr>
 
<tr>
−
<td>&nbsp;</td>
+
<th>&nbsp;</th>
−
<td>&nbsp;</td>
+
<th>&nbsp;</th>
−
<td>&nbsp;</td>
+
<th>&nbsp;</th>
−
<td>&nbsp;</td>
+
<th>&nbsp;</th>
−
<td>1</td>
+
<th>&nbsp;</th>
−
<td>2</td>
+
<th>&nbsp;</th>
−
<td>3</td>
+
<th>1</th>
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
−
<td>4</td>
+
<th>2</th>
−
<td>5</td>
+
<th>3</th>
−
<td>6</td>
+
<th>4</th>
−
<td>7</td>
+
<th>5</th>
−
<td>8</td>
+
<th>6</th>
−
<td>9</td>
+
<th>7</th>
−
<td>10</td>
+
<th>8</th>
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
−
<td>11</td>
+
<th>9</th>
−
<td>12</td>
+
<th>10</th>
−
<td>13</td>
+
<th>11</th>
−
<td>14</td>
+
<th>12</th>
−
<td>15</td>
+
<th>13</th>
−
<td>16</td>
+
<th>14</th>
−
<td>17</td>
+
<th>15</th>
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
−
<td>18</td>
+
<th>16</th>
−
<td>19</td>
+
<th>17</th>
−
<td>20</td>
+
<th>18</th>
−
<td>21</td>
+
<th>19</th>
−
<td>22</td>
+
<th>20</th>
−
<td>23</td>
+
<th>21</th>
−
<td>24</td>
+
<th>22</th>
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
−
<td>25</td>
+
<th>23</th>
−
<td>26</td>
+
<th>24</th>
−
<td>27</td>
+
<th>25</th>
−
<td>28</td>
+
<th>26</th>
−
<td>29</td>
+
<th>27</th>
−
<td>30</td>
+
<th>28</th>
−
<td>31</td>
+
<th>29</th>
 
</tr>
 
</tr>
  
 +
<tr>
 +
<th>30</th>
 +
<th>31</th>
 +
<th>&nbsp;</th>
 +
<th>&nbsp;</th>
 +
<th>&nbsp;</th>
 +
<th>&nbsp;</th>
 +
<th>&nbsp;</th>
 
</table>
 
</table>
  

Revision as of 10:53, 17 July 2015


iGEM Kent 2015

Notebook

  • June,2015
    • Sun Mon Tue Wed Thu Fri Sat
        1 2 3 4 5 6
      7 8 9 10 11 12 13
      14 15 16 17 18 19 20
      21 22 23 24 25 26 27
      28 29 30        
  • July,2015
    • Sun Mon Tue Wed Thu Fri Sat
            1 2 3 4
      5 6 7 8 9 13 14 15 16 17 18
      19 20 21 22 23 24 25
      26 27 28 29 30 31  
  • August 2015
    • Sun Mon Tue Wed Thu Fri Sat
                  1
      2 3 4 5 6 7 8
      9 10 11 12 13 14 15
      16 17 18 19 20 21 22
      23 24 25 26 27 28 29
      30 31          



















Day 1 Wet lab 22/06/15

  • Made LB plates
  • Made LB broth
  • TIPS autoclaved

    • Day 2 Wet lab 23/06/15

  • Set up Top10 overnight, VS45 overnight, US348 overnight
  • Meeting with advisors to discuss progress

    • Day 3 Wet lab 24/06/15

  • Filter sterilise the buffer
  • 250ml no antibiotic broth, put top10 cells in
  • Incubate until optical density is 0.6
  • Miniprep PSB1C3 plasmid

    • Day 4 Wet lab 25/06/15

  • Transformation
  • Prepare SBC media
  • Digest of PSB1c3 from MS348

    • Day 5 Wet lab 26/06/15

  • Calculated competent cell efficiency
  • Agarose gel formation
  • Gel electrophoresis

    • Day 6 Wet lab 29/06/15

    Transforming linear PSB1C3

    Day 7 Wet lab 30/06/15

  • Miniprep of PSB1A3 plasmid
  • Gel electrophoresis of a large quantity of PSB1C3
  • Meeting with advisors to discuss progress

    • Day 8 Wet lab 01/07/15

  • Gel electrophoresis of the purified DNA extracted
  • Transformation of pSB1A3 circular plasmid to VS45 cells (competent)
  • Digest of all pSB1C3 plasmids

    • Day 9 Wet lab 02/07/15

  • Competent cells transformation efficiency
  • Mini-prep of 1MS340 to get pSB1C3 plasmid
  • Gel extraction of big digest
  • Quantification of the digest. Added sample buffer to each digest

    • Day 10 Wet lab 03/07/15

  • Transformation efficiency

    • Day 11 Wet lab 06/07/15

  • TOP10 cells containing pSB1A3 with limonene synthase were cultured overnight on AMP plates
  • Colonies of VS45 containing pSB1A3 were patched onto chloramphenicol plates
  • Overnight digest of miniprepped pSBIC3 using ECORI and PSTl

    • Day 12 Wet lab 07/07/15

  • Agarose gel of overnight digest - no bands were visible
  • Set up overnight cultures of pSBIC3 and pSBIA3 to be miniprepped the next day
  • Set up an overnight digest of pSBIC3

    • Day 13 Wet lab 08/07/15

  • Agarose gel of overnight digest - no bands were visible
  • Miniprep of pSBIA3 and pSBIC3
  • Overnight digest of pSBIA3 and pSBIC3

    • Day 14 Wet lab 09/07/15

  • Agarose gel of digested pSBIA3 and pSBIC3 - no bands were visible
  • Overnight cultures of MS349 containing pSBIA3 LIMS and MS340 containing pSBIC3 were set up, ready to be miniprepped

    • Day 15 Wet lab 10/07/15

  • Miniprep of plasmids pSBIA3 and pSBIC3

    • Day 16 Wet lab 13/07/15

  • Overnight cultures of MS349 containing pSBIA3 LIMS and MS340 containing pSBIC3 were set up, ready to be miniprepped
  • Overnight digest of miniprepped plasmids pSBIA3 and pSBIC3 using the enzymes ECORI and PSTI

    • Day 17 Wet lab 14/07/15

  • Agarose gel of digested plasmids pSBIA3 and pSBIC3 - it finally worked!!!
  • Miniprep of overnight cultures
  • Gel extraction of both plasmids
  • Analytical gel using Hyperladder I to quantify the plasmid
  • Overnight cultures of Top10 cells containing PVS72

    Day 18 Wet lab 15/07/15

  • Miniprepped PVS72
  • Transformation of VS45 with PVS72
  • Made LB plates with Chloramphenicol and Ampicillin
  • Plated the transformed VS45 cells on the Chloramphenicol/Ampicillin plates
  • Agarose gel of leftover digested pSBIA3 and pSBIC3 from 14/07/15
  • Gel extraction of pSBIA3 and pSBIC3

    • Day 19 Wet lab 16/07/15

  • Counted the overnight colonies of VS45 with PVS72
  • Calculated the transformation efficiency