Difference between revisions of "Team:NAIT Edmonton/Trial11"

(Blanked the page)
Line 1: Line 1:
 +
{{NAIT_Edmonton/CSS2}}
 +
<html>
  
 +
<head>
 +
 +
 +
<title>Team NAIT 2015</title>
 +
 +
<link href='http://fonts.googleapis.com/css?family=Source+Sans+Pro' rel='stylesheet' type='text/css'>
 +
<script type="text/javascript" src="https://ajax.googleapis.com/ajax/libs/jquery/1.6.2/jquery.min.js"></script>
 +
<script type="text/javascript" src="https://2015.igem.org/index.php?title=
 +
Template:NAIT_Edmonton/js/jquery.flexslider"></script>
 +
 +
<script type="text/javascript" charset="utf-8">
 +
 +
var $ = jQuery.noConflict();
 +
  $(window).load(function() {
 +
    $('.flexslider').flexslider({
 +
          animation: "slide"
 +
    });
 +
  });
 +
</script>
 +
 +
</head>
 +
 +
<body>
 +
 +
 +
 +
<div id="header">
 +
<div class="header">
 +
 +
<style type="text/css">
 +
 +
.header_mobile {
 +
    display:none;
 +
    position: absolute;
 +
    background-color:transparent;
 +
    width:65px;
 +
    top: 90px;
 +
    left:50px;
 +
    height:65px;
 +
    z-index:999;
 +
    position:fixed;}
 +
 +
.header_mobile ul {list-style:none; position:absolute;float:right;margin:0;padding-right:125px}
 +
.header_mobile ul * {margin:0; padding:0;}
 +
.header_mobile a {display:block;color:#159CD8;text-decoration:none;padding:15px 50px}
 +
.header_mobile li {position:relative;float:left; padding:5px 5px 5px 5px}
 +
.header_mobile li a {font-family:'Source Sans Pro', sans-serif;color:#ffffff;font-size:20px;}
 +
.header_mobile li.current-menu-item {background:#5AB3E3}
 +
.header_mobile ul ul{display:none; position:absolute; top:77%; left:30%; background:#0D4D8C; padding:0 0px 0 0}
 +
.header_mobile ul ul li:hover{background:#159CD8}
 +
.header_mobile ul ul li{float:none; width:120px}
 +
.header_mobile ul ul a {line-height:100%; padding:0 20px 0 0; font-size:15px }
 +
.header_mobile ul ul ul {top:0; left:200%}
 +
.header_mobile ul li.selected a{color:#8BC5EB}
 +
.header_mobile ul li:hover > ul{display:block;}
 +
 +
 +
@media screen and (max-width:1000px){
 +
 +
.header_left{display:none;}
 +
.header_right{display:none;}
 +
.header_mobile{display:block;}
 +
 +
}
 +
 +
</style>
 +
 +
    <div class="logo"><a href="https://2015.igem.org/Team:NAIT_Edmonton">
 +
            <img style="padding-left:6px" src="https://static.igem.org/mediawiki/2015/c/c7/NAIT_IGEM_Separated.png" height="185px"></a>
 +
    </div>
 +
    <div class="header_left">
 +
        <ul>
 +
               
 +
                <li><a href="https://2015.igem.org/Team:NAIT_Edmonton/Team">team</a>
 +
                    <ul>
 +
                      <li><a href="https://2015.igem.org/Team:NAIT_Edmonton/Bios">bios</a></li>
 +
                      <li><a href="https://2015.igem.org/Team:NAIT_Edmonton/Attributions">attributions</a></li>
 +
                      <li><a href="https://igem.org/Team.cgi">official team profile</a>
 +
                    </ul>
 +
                </li>
 +
                <li><a href="https://2015.igem.org/Team:NAIT_Edmonton/Project">project</a>
 +
                    <ul>
 +
                    <li><a href="https://2015.igem.org/Team:NAIT_Edmonton/Desc">description</a></li>
 +
                      <li><a href="https://2015.igem.org/Team:NAIT_Edmonton/Protocols">experiment and protocols</a></li>
 +
                      <li><a href="https://2015.igem.org/Team:NAIT_Edmonton/Results">parts and results</a></li>
 +
                      <li><a href="https://2015.igem.org/Team:NAIT_Edmonton/Modeling">modeling</a></li>
 +
                    </ul>
 +
                </li>
 +
            </ul>
 +
    </div>
 +
    <div class="header_right">
 +
        <ul>
 +
                <li><a href="https://2015.igem.org/Team:NAIT_Edmonton/Human">human practices</a>
 +
                <ul>
 +
                    <li><a href="https://2015.igem.org/Team:NAIT_Edmonton/Outreach">community outreach</a></li>
 +
                      <li><a href="https://2015.igem.org/Team:NAIT_Edmonton/Practices">policy and practices</a></li>
 +
                      <li><a href="https://2015.igem.org/Team:NAIT_Edmonton/Collaborations">collaborations</a></li>
 +
                      <li><a href="https://2015.igem.org/Team:NAIT_Edmonton/Entrepreneurship">entrepreneurship</a></li>
 +
                    </ul>
 +
                </li>
 +
                <li><a href="https://2015.igem.org/Team:NAIT_Edmonton/Notebook">notebook</a>
 +
                <ul>
 +
                    <li><a href="https://2015.igem.org/Team:NAIT_Edmonton/Safety">lab safety</a></li>
 +
                      <li><a href="https://2015.igem.org/Team:NAIT_Edmonton/Achievements">achievements</a></li>
 +
                      <li><a href="https://2015.igem.org/Team:NAIT_Edmonton/Logbook">log book</a></li>
 +
                </ul>
 +
                </li>
 +
        </ul>
 +
</div>
 +
</div>
 +
 
 +
 +
  <div id="wrap">
 +
  <div class="desc">
 +
 +
<style type="text/css">
 +
 +
.top_slogan {text-align:center; font-family: 'Source Sans Pro', sans-serif; color:#0D4D8C; font-size:30px; padding: 40px 0px 40px 0px; font-style:strong; line-height:40px;}
 +
 +
</style>
 +
 +
<center><div class="top_slogan">The Project</div></center>
 +
 +
<h1>Background</h1></a>
 +
 +
<p style="font-size:15px">The structural and functional study of the proteins expressed by a genome is
 +
 +
called proteomics. This relatively novel science uses different methodologies in order to
 +
 +
separate and identify specific proteins of interest. Among these techniques, SDS-PAGE
 +
 +
plays an essential role due to its high sensitivity, low sample volume requirement, and
 +
 +
high popularity. Negatively charged proteins migrate towards the positive electrode
 +
 +
according to their size and charge. Smaller proteins migrate further in a given amount of
 +
 +
time. As proteins are separated in this manner, users load molecular weight standards
 +
 +
to estimate the size (in kDa) of the proteins present in their sample. Once the proteins of
 +
 +
a single sample have been isolated and are embedded in the polyacrylamide (PA) gel
 +
 +
matrix, staining procedures are used to visualize them.</p>
 +
 +
<br>
 +
 +
<center><img src="http://upload.wikimedia.org/wikipedia/commons/4/46/SDS-PAGE_Electrophoresis.png" width="750px"></center>
 +
 +
<br>
 +
 +
<p style="font-size:15;">Organic dyes, such as Coomassie blue, can be used for this purpose;
 +
 +
nevertheless, their low sensitivity and a detection range that goes from 1 to 50 ng can
 +
 +
be a challenge for detecting low abundance proteins (Jin, Huang, Yoo, & Choi, 2006). A
 +
 +
higher sensitivity can be achieved by fluorescent staining techniques (from 0.1 to 10
 +
 +
ng.); however, UV instruments are necessary in order to read the data (Jin et al., 2006).
 +
 +
The most sensitive method up to date is radiolabeling, but the requirement of hazardous
 +
 +
isotopes and their complex management makes it a complicated procedure (Jin et al.,
 +
 +
2006). Silver staining is a method that offers great sensitivity and an easy to handle
 +
 +
protocol, thus making it one of the most commonly used staining methods. </p>
 +
 +
<br>
 +
 +
<center><img src="http://www.bio-rad.com/webroot/web/images/lsr/products/electrophoresis/product_overlay_content/global/lsr_biosafe_coomasie_gel.jpg"></center>
 +
 +
<br><br>
 +
 +
<h1>The Problem </h1></a>
 +
 +
<p style="font-size:15px">Difficulties with silver staining arise when the molecular weight markers are re-
 +
 +
colored golden-brown in the staining process. Markers offer evenly distributed proteins
 +
 +
that show bands of equal intensity and known size. Researchers can compare these
 +
 +
bands with their sample and identify the protein they are looking for based on its size. A
 +
 +
subset of these markers has color-coded standard proteins to facilitate the identification
 +
 +
of each band. Post-silver staining, the users lose the ability to use the color code as a
 +
 +
reference.</p>
 +
 +
<br>
 +
 +
<center><img src="http://labs.mmg.pitt.edu/gjoerup/silver_stain.jpg" width="750px"></center>
 +
     
 +
  </div>
 +
  </div>
 +
 +
  <div class="clear"></div>
 +
 +
 +
<div class="header_mobile">
 +
  <ul>
 +
        <li><a href="https://2015.igem.org/Team:NAIT_Edmonton/MobileMenu">menu</a>
 +
          <ul>
 +
              <li><a href="https://2015.igem.org/Team:NAIT_Edmonton/Team">team</a>
 +
              <li><a href="https://2015.igem.org/Team:NAIT_Edmonton/Project">project</a>
 +
              <li><a href="https://2015.igem.org/Team:NAIT_Edmonton/Human">human practices</a>
 +
              <li><a href="https://2015.igem.org/Team:NAIT_Edmonton/Notebook">notebook</a>
 +
          </ul>
 +
        </li>   
 +
           
 +
  </ul>
 +
</div>
 +
 
 +
 
 +
</div>
 +
 +
 +
 +
        <div class="footer">
 +
        <div class="footer_content">
 +
            <div class="footer_left">
 +
                        <ul class="footer_menu">
 +
                    <li><a href="https://2015.igem.org/Team:NAIT_Edmonton">home</a></li>
 +
                    <li><a href="https://2015.igem.org/Team:NAIT_Edmonton/Team">team</a></li>
 +
                    <li><a href="https://2015.igem.org/Team:NAIT_Edmonton/Project">project</a></li>
 +
                    <li><a href="https://2015.igem.org/Team:NAIT_Edmonton/Human">human practices</a></li>
 +
                    <li><a href="https://2015.igem.org/Team:NAIT_Edmonton/Notebook">notebook</a></li>
 +
                </ul>
 +
            </div>
 +
           
 +
            <div class="footer_right">
 +
                <ul class="social_icons">
 +
                <li><a href="http://www.facebook.com/pages/IGEM-2015-NAIT-Edmonton/884025281659292"><img src="https://static.igem.org/mediawiki/2015/a/a3/NAIT_Icon_facebook.png" alt="" title="" /></a></li>
 +
                <li><a href="http://www.twitter.com/TeamNAIT2015"><img src="https://static.igem.org/mediawiki/2015/6/64/NAIT_Icon_twitter.png" alt="" title="" /></a></li>
 +
                </ul>
 +
            </div>
 +
          <div class="clear"></div>
 +
          </div>
 +
        </div>
 +
</div>
 +
 +
<style type="text/css">
 +
 +
#footer {z-index:15;}
 +
.footer {position: relative; padding:20px 0 40px 0; margin: -25px 0 0 0; clear:both; width:100%; background-color:#0D4D8C; height:25px;}
 +
.footer_content {margin:auto;width:100%;}
 +
.footer_left { float:left; padding:0 0 0 10%;}
 +
.footer_right{ float:right; padding:0 10% 0 0;}
 +
ul.social_icons { margin:0px; padding:0px; list-style:none;}
 +
ul.social_icons li{ float:left;}
 +
ul.social_icons li a{width:30px;height:30px;display:block;border-radius:15px;-moz-border-radius:15px;-webkit-border-radius:15px;-khtml-border-radius:15px;float:left;margin:0px 0 0px 5px;background-color:#ADD3F0;text-align:center;}
 +
ul.social_icons li a#top{width:30px;height:30px;display:block;border-radius:15px;-moz-border-radius:15px;-webkit-border-radius:15px;-khtml-border-radius:15px;float:left;margin:0px 0 0px 5px;background-color:#ADD3F0;text-align:center;}
 +
ul.social_icons li a:hover{ background-color:#5AB3E3;}
 +
ul.social_icons li a img{ width:60%; display:block; margin:5px auto;}
 +
 +
ul.footer_menu{ padding:10px 0 0 0; margin:0px; list-style:none;}
 +
ul.footer_menu li{ float:left; padding:0 15px 0 0;}
 +
ul.footer_menu li a{ background:url(https://static.igem.org/mediawiki/2015/1/17/NAIT_Bullet.png) no-repeat left; padding:0 0 0 12px;}
 +
 +
 +
</style>
 +
 +
</body>
 +
 +
<!----------
 +
 +
                          _ __  _
 +
                        ;'  '',)
 +
                        /;6 , ;/
 +
                        (Y)_:., |
 +
                        `-', :; \
 +
                          |;  ,.:\
 +
                          /:.;  ;;)
 +
                        |:;,.'|  :/
 +
                        / |:  / ; /
 +
                      /:;\ `| "//
 +
                      /_,: | |./,|
 +
                    _/: \.'|,|/| |
 +
                  /:.,:.|,|"| |:|
 +
                  /:;:|:,/;|:| |'|
 +
        eduardo  |',:| \_ \ |_|;\_
 +
                /;\_ /\_)) \_))\_))
 +
                (;(________
 +
                '''''`'''~`
 +
 +
------------------>
 +
 +
 +
</html>

Revision as of 16:52, 30 July 2015

Team NAIT 2015