Difference between revisions of "Team:Aalto-Helsinki"
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<p>Climate change is argued to be one of the greatest challenges faced by mankind. Its primary cause is believed to be man-made CO2 emissions from transportation and electricity production. To tackle the issue of transportation emissions, we want to produce sustainable propane in Escherichia coli using cellulosic feedstock. The pathway is a patchwork of 10 different enzymes from different organisms, such as Mycobacterium marinum and Bacillus subtilis. We built a model of the pathway to identify its bottlenecks and concentrate our engineering efforts on them. To elevate our propane from a food crop -based first generation biofuel to the second generation, we are integrating a secretion system for cellulose hydrolysing enzymes. To innovatively enhance our production system, we are bringing the two final enzymes of our pathway into close proximity by fusing them with micelle-forming amphiphilic proteins. This increases theoretical yield, bringing us one step closer to commercially viable biopropane.</p> | <p>Climate change is argued to be one of the greatest challenges faced by mankind. Its primary cause is believed to be man-made CO2 emissions from transportation and electricity production. To tackle the issue of transportation emissions, we want to produce sustainable propane in Escherichia coli using cellulosic feedstock. The pathway is a patchwork of 10 different enzymes from different organisms, such as Mycobacterium marinum and Bacillus subtilis. We built a model of the pathway to identify its bottlenecks and concentrate our engineering efforts on them. To elevate our propane from a food crop -based first generation biofuel to the second generation, we are integrating a secretion system for cellulose hydrolysing enzymes. To innovatively enhance our production system, we are bringing the two final enzymes of our pathway into close proximity by fusing them with micelle-forming amphiphilic proteins. This increases theoretical yield, bringing us one step closer to commercially viable biopropane.</p> | ||
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Revision as of 08:23, 12 August 2015