Difference between revisions of "Team:Amoy/Notebook"
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font-weight: bold; | font-weight: bold; | ||
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<div style="width: 80%; margin-left: 10%;"> | <div style="width: 80%; margin-left: 10%;"> | ||
<p class="detail_h1">Purpose:</br></p> | <p class="detail_h1">Purpose:</br></p> | ||
| − | <p class="detail_p">Extract plasmid from dry powder | + | <p class="detail_p">Extract plasmid from dry powder</br></p> |
<p class="detail_h1">Steps:</br></p> | <p class="detail_h1">Steps:</br></p> | ||
<p class="detail_p">1. Add 20ul ddH20 to solve the dry powder</br> | <p class="detail_p">1. Add 20ul ddH20 to solve the dry powder</br> | ||
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<p class="detail_p"></br>3. Pick a single colony from the agar plate using a sterile pipette tip. </br> | <p class="detail_p"></br>3. Pick a single colony from the agar plate using a sterile pipette tip. </br> | ||
4. Culture at 10ml LB of chloramphenicol for 12h</br> | 4. Culture at 10ml LB of chloramphenicol for 12h</br> | ||
| − | 5. Plasmid minipre | + | 5. Plasmid minipre</br></p> |
<p class="detail_h1">Product:</br></p> | <p class="detail_h1">Product:</br></p> | ||
<p class="detail_p">plasmid of promoter J23100</br></p> | <p class="detail_p">plasmid of promoter J23100</br></p> | ||
| Line 94: | Line 95: | ||
<div style="width: 80%; margin-left: 10%;"> | <div style="width: 80%; margin-left: 10%;"> | ||
<p class="detail_h1">Purpose:</br></p> | <p class="detail_h1">Purpose:</br></p> | ||
| − | <p class="detail_p">Extract plasmid from dry powder | + | <p class="detail_p">Extract plasmid from dry powder</br></p> |
<p class="detail_h1">Steps:</br></p> | <p class="detail_h1">Steps:</br></p> | ||
<p class="detail_p">1. Add 20ul ddH20 to solve the dry powder</br> | <p class="detail_p">1. Add 20ul ddH20 to solve the dry powder</br> | ||
| Line 101: | Line 102: | ||
<p class="detail_p"></br>3. Pick a single colony from the agar plate using a sterile pipette tip. </br> | <p class="detail_p"></br>3. Pick a single colony from the agar plate using a sterile pipette tip. </br> | ||
4. Culture at 10ml LB of ampicillin for 12h</br> | 4. Culture at 10ml LB of ampicillin for 12h</br> | ||
| − | 5. Plasmid minipre | + | 5. Plasmid minipre</br></p> |
<p class="detail_h1">Product:</br></p> | <p class="detail_h1">Product:</br></p> | ||
<p class="detail_p">plasmid of promoter LacI</br></p> | <p class="detail_p">plasmid of promoter LacI</br></p> | ||
| Line 112: | Line 113: | ||
<div style="width: 80%; margin-left: 10%;"> | <div style="width: 80%; margin-left: 10%;"> | ||
<p class="detail_h1">Purpose:</br></p> | <p class="detail_h1">Purpose:</br></p> | ||
| − | <p class="detail_p">Extract plasmid from dry powder | + | <p class="detail_p">Extract plasmid from dry powder</br></p> |
<p class="detail_h1">Steps:</br></p> | <p class="detail_h1">Steps:</br></p> | ||
<p class="detail_p">1. Add 20ul ddH20 to solve the dry powder</br> | <p class="detail_p">1. Add 20ul ddH20 to solve the dry powder</br> | ||
| Line 119: | Line 120: | ||
<p class="detail_p"></br>3. Pick a single colony from the agar plate using a sterile pipette tip. </br> | <p class="detail_p"></br>3. Pick a single colony from the agar plate using a sterile pipette tip. </br> | ||
4. Culture at 10ml LB of ampicillin for 12h</br> | 4. Culture at 10ml LB of ampicillin for 12h</br> | ||
| − | 5. Plasmid minipre | + | 5. Plasmid minipre</br></p> |
<p class="detail_h1">Product:</br></p> | <p class="detail_h1">Product:</br></p> | ||
<p class="detail_p"> </br></p> | <p class="detail_p"> </br></p> | ||
| Line 130: | Line 131: | ||
<div style="width: 80%; margin-left: 10%;"> | <div style="width: 80%; margin-left: 10%;"> | ||
<p class="detail_h1">Purpose:</br></p> | <p class="detail_h1">Purpose:</br></p> | ||
| − | <p class="detail_p">Extract plasmid from dry powder | + | <p class="detail_p">Extract plasmid from dry powder</br></p> |
<p class="detail_h1">Steps:</br></p> | <p class="detail_h1">Steps:</br></p> | ||
<p class="detail_p"> | <p class="detail_p"> | ||
| Line 139: | Line 140: | ||
3. Pick a single colony from the agar plate using a sterile pipette tip. </br> | 3. Pick a single colony from the agar plate using a sterile pipette tip. </br> | ||
4. Culture at 10ml LB of ampicillin for 12h</br> | 4. Culture at 10ml LB of ampicillin for 12h</br> | ||
| − | 5. Plasmid minipre | + | 5. Plasmid minipre</br></p> |
<p class="detail_h1">Product:</br></p> | <p class="detail_h1">Product:</br></p> | ||
<p class="detail_p"> </br></p> | <p class="detail_p"> </br></p> | ||
| Line 150: | Line 151: | ||
<div style="width: 80%; margin-left: 10%;"> | <div style="width: 80%; margin-left: 10%;"> | ||
<p class="detail_h1">Purpose:</br></p> | <p class="detail_h1">Purpose:</br></p> | ||
| − | <p class="detail_p">Extract plasmid from dry powder | + | <p class="detail_p">Extract plasmid from dry powder</br></p> |
<p class="detail_h1">Steps:</br></p> | <p class="detail_h1">Steps:</br></p> | ||
<p class="detail_p"> | <p class="detail_p"> | ||
| Line 159: | Line 160: | ||
3. Pick a single colony from the agar plate using a sterile pipette tip. </br> | 3. Pick a single colony from the agar plate using a sterile pipette tip. </br> | ||
4. Culture at 10ml LB of chloramphenicol for 12h</br> | 4. Culture at 10ml LB of chloramphenicol for 12h</br> | ||
| − | 5. Plasmid minipre | + | 5. Plasmid minipre</br></p> |
<p class="detail_h1">Product:</br></p> | <p class="detail_h1">Product:</br></p> | ||
<p class="detail_p"> </br></p> | <p class="detail_p"> </br></p> | ||
Revision as of 06:08, 11 September 2015
NOTEBOOK
Initially, they used isolated enzymes, which can be disadvantageous for the reason that enzymes are always destabilized in the isolation and purification process. What's more, the cofactor-NADH is rather an expensive raw material, which will enhance the cost of L-tert-leucine production. So scientists introduced whole-cell biocatalysts to L-tert-leucine production. Whole-cell biocatalysts could stabilize enzymes and reduce the addition level of cofactor NADH. In the path of building our biobricks, we divided the circuits into two modules. One is promoter linked with rbs and the other is gene linked with terminator. The dendrogram below is our experiments detail. Click each bottom for more information.
CONTACT US
Email: igemxmu@gmail.com
Website: 2015.igem.org/Team:Amoy
Address: Xiamen University, No. 422, Siming South Road, Xiamen, Fujian, P.R.China 361005
