Difference between revisions of "Team:Tokyo-NoKoGen"

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<h2> Team Project Descriptions </h2>
 
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Revision as of 13:41, 12 July 2015

Team Project Descriptions

-Background-

Do you know serious pest damages that is harmful to human house and buildings…?

The criminal is one of the flagitious vermin….termites.

Termites are insects that feed on wood and paper, and eat like pillars of houses or books. There are a lot of pesticides developed to exterminate termites. However, many of the pesticides against termites is an organophosphorus agent which has an immediate effect to the individual, while has a negative effect on the human body.


To solve this problem of pesticides, NoKogen aim to engineer new termites extermination system in iGEM project.

-Object-

We have two objectives to realize our project.
・Exterminate termite in slow-acting method on a long-term basis
・Create a new pesticide which is not harmful to man

-Method-

To achieve these objective, we are engineering the E. coli which produce poison against termites and work in termite’s guts.
Also, we focused on the two properties of termites.
First , the main component of the blood glucose of termites is trehalose, and trehalose used as the primary energy source.
Actually, in previous year, NoKoGen engineered E. coli which has ability to kill cockroaches. Cockroaches and termites are the same order so we thought that we can apply the previous project to solve this problems.
In concrete method, we are planning to use 3,3-diketotrehalose. it is an inhibitor of trehalase which converts trehalose to glucose. Cockroach or termites digested carbohydrates and metabolized to trehalose. Trehalose stored in the blood and is taken up by cells additionally converts trehalose to glucose 3,3-diketotrehalose is produced by the enzymatic reaction of glucose 3 dehydrogenase.
We aim to express the G3DH in E. coli and prevent the trehalase activity by competitive inhibition.
Moreover, we construct OtsA and OtsB which convert glucose to trehalose.
Many more trehalose is converted to 3,3-diketotrehalose which inhibits termite’s passway.

Second properties of termites is that termites carry and lick their eggs.
Termites recognize their egg as spherical material has a diameter of 0.5 mm and pheromone. Based on this feature of termites, we try to make the pseudo-eggs to use the habit.
We are planning to make pseudo-eggs with LB agar which is cut to the spherical and engineered E. coli in it. We will treat this material as pseudo egg and apply the Lysozyme as termite’s pheromone so that termites recognize them as eggs and take them to termites colony.
We expect that this pseudo-eggs are effective to exterminate termites.

-Biosafety-

-Human practice-

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