Difference between revisions of "Team:Warwick/Home"

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<script>/*
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<script>/
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*
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* conditions and the following disclaimer.
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* of conditions and the following disclaimer in the documentation and/or other materials
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*
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*
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* THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY
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* EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF
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* MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE
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*
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*
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*
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* Redistribution and use in source and binary forms, with or without modification,
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* are permitted provided that the following conditions are met:
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*
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* Redistributions of source code must retain the above copyright notice, this list of
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* conditions and the following disclaimer.
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* Redistributions in binary form must reproduce the above copyright notice, this list
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* of conditions and the following disclaimer in the documentation and/or other materials
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* provided with the distribution.
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*
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* Neither the name of the author nor the names of contributors may be used to endorse
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*
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* THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY
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* EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF
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*
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* Copyright 2011 @louis_remi
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* Licensed under the MIT license.
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*/
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/*
 
* jQuery Nivo Slider v3.1
 
* http://nivo.dev7studios.com
 
*
 
* Copyright 2013, Dev7studios
 
* Free to use and abuse under the MIT license.
 
* http://www.opensource.org/licenses/mit-license.php
 
*/
 
  
 
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Revision as of 10:32, 5 August 2015

Warwick iGEM 2015

  • Dare to

    Dream

  • image02

    Dare to

    Hope

  • Dare to

    Smile

  • image04

    Dare to

    ask

  • image05

    Dare to

    refuse

  • image06

    Dare to

    give

  • image07

    Dare to

    receive

  • image08

    Dare to

    Love

  • image09

    Dare to

    Forgive

  • image10

    Warwick iGEM

    2015

"Warwick iGEM 2015 is the greatest team in the world" - Winston Churchill

At Warwick iGEM we are passionate about the art of science, the way we can meld biology with design to make a product greater than the sum of its parts. We were inspired by this paper on using zinc fingers to barcode cells with fluorescently tagged double stranded DNA oligos; and considered whether it was possible to use these same zinc finger proteins in a way that has never been done before. By modifying our cells to express zinc finger binding domains on the outside of the cell wall could we, in the same manner that the DNA is bound to the cells in the paper, instead bind the cells to fixed structured DNA on a 2D plane? And then, using existing DNA origami research, could we expand this into creating self assembling 3D cell structures.

Fluorescent microscopy has long been the go to method for validating protein expression, and we are carrying on this long tradition by tying the expression of cell surface zinc fingers with the expression of fluorescent proteins inside the cell. By using multiple different zinc fingers with unique specific binding sites we allow different cell types to bond simultaneously to our DNA structure. The potential applications of this are limitless, imagine layers of different types of synthetic tissues; the construction of lab made skin and other organs. Imagine also a world where advanced materials with enhanced properties made cheaply by multiple different cells working together is possible.

As a proof of concept, that it is possible to bind different cell types to the same structure we are engineering E. coli to express different coloured fluorescent proteins along with their unique zinc finger proteins, showing visually how many different coloured cells can coexist in a controlled environment. Coincidentally, this allows us also to create art by arranging groups of coloured cells into images, allowing for some of the first fully coloured images made using only living cells.