Difference between revisions of "Team:UCLA/Notebook/Honeybee Silk/17 May 2015"
(→Growing Colonies) |
(→Growing Cultures) |
||
Line 4: | Line 4: | ||
==Growing Cultures== | ==Growing Cultures== | ||
*Prepped 2 tubes of LB(5mL) with chloremphenicol and picked colonies of Bba-xxxx[http://benchling.com/dcancilla/f/mVVgFLnR-honeybee-silk-sequences/seq-cc4djRXz-promsilk-in-psb1c3/edit] to generate a starter culture for protein expression. Also prepped 2 tubes of of LB(5mL) with kanomycin and picked colonies of PET24a in order to miniprep and generate more plasmid. | *Prepped 2 tubes of LB(5mL) with chloremphenicol and picked colonies of Bba-xxxx[http://benchling.com/dcancilla/f/mVVgFLnR-honeybee-silk-sequences/seq-cc4djRXz-promsilk-in-psb1c3/edit] to generate a starter culture for protein expression. Also prepped 2 tubes of of LB(5mL) with kanomycin and picked colonies of PET24a in order to miniprep and generate more plasmid. | ||
+ | *We are growing the starter culture for the silk protein up to an OD of 0.8. | ||
+ | **Because we started the incubation at 3pm, and we dont want to overgrow, we will put the cells in the 30 C shaker until 10 pm, at which point we will transfer to the 37 degree shaker. | ||
+ | **We will transfer the starter culture to a larger volume tomorrow morning 5/18 |
Revision as of 22:43, 17 May 2015
May 17, 2015
Growing Cultures
- Prepped 2 tubes of LB(5mL) with chloremphenicol and picked colonies of Bba-xxxx[http://benchling.com/dcancilla/f/mVVgFLnR-honeybee-silk-sequences/seq-cc4djRXz-promsilk-in-psb1c3/edit] to generate a starter culture for protein expression. Also prepped 2 tubes of of LB(5mL) with kanomycin and picked colonies of PET24a in order to miniprep and generate more plasmid.
- We are growing the starter culture for the silk protein up to an OD of 0.8.
- Because we started the incubation at 3pm, and we dont want to overgrow, we will put the cells in the 30 C shaker until 10 pm, at which point we will transfer to the 37 degree shaker.
- We will transfer the starter culture to a larger volume tomorrow morning 5/18