Difference between revisions of "Team:UCLA/Notebook/Honeybee Silk/11 May 2015"

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Revision as of 16:30, 5 June 2015

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May 11 Sequencing of silk biobricks

Today we sent in both our constructs to GeneWiz for sequencing.

  • For sequencing primers, I used the igem vf2 primers and vr primers that bind to the psb1c3 backbone, as well as a sequencing primer that we designed to bind to the silk sequence (p13) see https://benchling.com/s/p7bClpzQ/edit
  • The sequencing results came back and checked out for sample 1 of construct 1 (silk coding region) and samples 1 and 2 for construc 2 (regulatory elements + silk) i.e. we have our first honeybee biobricks!!!
  • For sequencing results of construct 1 see https://benchling.com/s/4XT6VRRU/edit (click on alignments on the right side of screen)
  • For sequencing results of construct 2 see https://benchling.com/s/TxD8z7Kq/edit (click on alignments on the right side of screen)