Difference between revisions of "Team:NAIT Edmonton/Results"
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<section id="content1"> | <section id="content1"> | ||
| − | <p> | + | <p> pSB1C3 is a high copy number plasmid (RFC [10]) carrying chloramphenicol resistance. </p> <br> |
| − | + | ||
| − | </p> <br> | + | <p> The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). </p> <br> |
| − | <p> | + | |
| − | + | <p> pSB1C3 has terminators bracketing its MCS which are designed to prevent transcription from *inside* the MCS from reading | |
| − | + | out into the vector. The efficiency of these terminators is known to be < 100%. Ideally we would construct a future set | |
| + | of terminators for bracketing a MCS that were 100% efficient in terminating both into and out of the MCS region.</p> <br> | ||
</section> | </section> | ||
Revision as of 17:26, 9 September 2015
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pSB1C3 Backbone
All parts for iGEM are required to submitted in the pSB1C3 backbone. As such, all of our custom sequences were ligated with this particular backbone.
pSB1C3 is a high copy number plasmid (RFC [10]) carrying chloramphenicol resistance.
The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
pSB1C3 has terminators bracketing its MCS which are designed to prevent transcription from *inside* the MCS from reading out into the vector. The efficiency of these terminators is known to be < 100%. Ideally we would construct a future set of terminators for bracketing a MCS that were 100% efficient in terminating both into and out of the MCS region.
Bacon ipsum dolor sit amet landjaeger sausage brisket, jerky drumstick fatback boudin ball tip turducken. Pork belly meatball t-bone bresaola tail filet mignon kevin turkey ribeye shank flank doner cow kielbasa shankle. Pig swine chicken hamburger, tenderloin turkey rump ball tip sirloin frankfurter meatloaf boudin brisket ham hock. Hamburger venison brisket tri-tip andouille pork belly ball tip short ribs biltong meatball chuck. Pork chop ribeye tail short ribs, beef hamburger meatball kielbasa rump corned beef porchetta landjaeger flank. Doner rump frankfurter meatball meatloaf, cow kevin pork pork loin venison fatback spare ribs salami beef ribs.
Jerky jowl pork chop tongue, kielbasa shank venison. Capicola shank pig ribeye leberkas filet mignon brisket beef kevin tenderloin porchetta. Capicola fatback venison shank kielbasa, drumstick ribeye landjaeger beef kevin tail meatball pastrami prosciutto pancetta. Tail kevin spare ribs ground round ham ham hock brisket shoulder. Corned beef tri-tip leberkas flank sausage ham hock filet mignon beef ribs pancetta turkey.
Bacon ipsum dolor sit amet beef venison beef ribs kielbasa. Sausage pig leberkas, t-bone sirloin shoulder bresaola. Frankfurter rump porchetta ham. Pork belly prosciutto brisket meatloaf short ribs.
Brisket meatball turkey short loin boudin leberkas meatloaf chuck andouille pork loin pastrami spare ribs pancetta rump. Frankfurter corned beef beef tenderloin short loin meatloaf swine ground round venison.
