Difference between revisions of "Team:SDU-Denmark/Tour32"
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<h1> Design </h1> | <h1> Design </h1> | ||
<h2>T25 </h2> | <h2>T25 </h2> | ||
| − | <p> The constructs LacZ promotor, is induced by IPTG and inhibited by LacI and CAP. It’s followed by a ribosomal binding site. The coding region is KT25 subunit of adenylate cyclase, followed by a linker, the wanted target protein and a terminator. | + | <p> The constructs LacZ promotor, is induced by IPTG and inhibited by LacI and CAP. It’s followed by a ribosomal binding site. The coding region is KT25 subunit of adenylate cyclase, followed by a linker, the wanted target protein and a terminator. T25 origins from Bordetella pertussis. Backbone for the construct is pSB1K3. </p> |
<h2>T18 </h2> | <h2>T18 </h2> | ||
| − | <p> The same promoter regulates this construct as with | + | <p> The same promoter regulates this construct as with T25. After the promoter, it contains a ribosomal binding site, the other subunit of Bordetella pertussis adenylate cyclase T18, a linker, the scaffold human thioredoxin containing a XhoI site, and a 3xFLAG tag. pSB1C3 is this constructs backbone. </p> |
Revision as of 21:36, 9 September 2015
Design
T25
The constructs LacZ promotor, is induced by IPTG and inhibited by LacI and CAP. It’s followed by a ribosomal binding site. The coding region is KT25 subunit of adenylate cyclase, followed by a linker, the wanted target protein and a terminator. T25 origins from Bordetella pertussis. Backbone for the construct is pSB1K3.
T18
The same promoter regulates this construct as with T25. After the promoter, it contains a ribosomal binding site, the other subunit of Bordetella pertussis adenylate cyclase T18, a linker, the scaffold human thioredoxin containing a XhoI site, and a 3xFLAG tag. pSB1C3 is this constructs backbone.