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Revision as of 16:14, 11 September 2015

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JOURNAL

  Hi! Here recorded the “significant” stages of our team which we experienced throughout the whole summer. In the initial establishment of our team, we organized various social activities and uneasy demonstrated the project at the first time, which all presents to you here.

Journal

March 24th

The establishment of our team!

April 9th

We established the iGEM club in our campus.

April 20th

Determined the project as "magnetotactic E.coli generates electronics."

May 8th

Communicated with professor Hu from Hong Kong University Medicine School.

May 10th

Determined three vectors for co-transferred which are pACYCDuet-1, pET28a and pCDFDuet-1.

June 12th

Amplified the mamGFDC and mms6 with PCR from MSR-1.

June 15th

Linearization of the pACYCDuet-1 and amplification of the mamW, RFP and laccase.

June 18th

Presentation and science lesson at the experimental Kindergarden Affiliated to UESTC.

June 19th

Presentation and Interaction at the Primary School Affiliated to UESTC

July 15th

Five teaching teams’ from iGEM club departure towards schools in remote area.

July 21th

The start of the Summer Training Camp.

July 25th

The establishment of the iGEM Southwest Union of China, also the first meet up.

July 27th

We transferred mamW, RFP and laccase gene into BL21 and induced its expression.

July 30th

Connected mamAB with the vector pET28a and complete the construction of vector piGEM-pET28a-mamAB.

August 5th

Amplified the mamXY with PCR from MSR-1. Completed the construction of vector piGEM-pET28a-mamAB.

August 12th

Communication with technical expert at Anno Medical Devices Co.Ltd.

August 13th

Detection of laccase activity using by the ABTS. Communication with Manager Zhiyong Xu at Jin Douyun Space Company.

August 17th

Finished the project video.

August 23th

Connected mamXY with the vector pCDFDuet-1 and connected mamGFDC + mms6 with the vector pCDFDuet-1-mamXY.

August 25th

We visited Chengdu Biology Maker Space and got their technical support.

August 29th

We were excited to obtain the exact Parts including the mamW gene and mutanted it.

September 10th

It is an exciting day that we sent our Parts to MIT, wish the little box bon voyage. Happy to complete the construction of cell device and start to test it.

September 23th

Set out for 2015 iGEM Giant Jamboree in MIT. We are coming, we are CHINA_CD_UESTC!