Difference between revisions of "Team:TCU Taiwan/Basic Part"
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<td><a href="http://parts.igem.org/Part:BBa_K1727001">BBa_K1727001</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K1727001">BBa_K1727001</a></td> | ||
<td>Coding</td> | <td>Coding</td> | ||
| − | <td><a title="Epinecidin-1 is a kind of antimicrobial peptide (AMPs) and it is a stable peptides that have extensive abilities to kill or inhibit the growth of bacteria. They play a role in defense mechanism for Epinephelus coioides to against microbes. Epinecidin-1 use it's chargeability to interact with bacteria cell membrane. Than use hydrophobic region interfere the membrane structure. This leads to cell lysis and bypasses bacterial antibiotic drug-resistance mechanisms. Moreover Epinecidin-1 has ability to help wound healing and has been proven by animal studies." href="">Epinecidin-1</a></td> | + | <td><a title="Epinecidin-1 is a kind of antimicrobial peptide (AMPs) and it is a stable peptides that have extensive abilities to kill or inhibit the growth of bacteria. They play a role in defense mechanism for Epinephelus coioides to against microbes. Epinecidin-1 use it's chargeability to interact with bacteria cell membrane. Than use hydrophobic region interfere the membrane structure. This leads to cell lysis and bypasses bacterial antibiotic drug-resistance mechanisms. Moreover Epinecidin-1 has ability to help wound healing and has been proven by animal studies." href="">Epinecidin-1 (<I>Epinephelus coioides</I>)</a></td> |
<td>216 bp</td> | <td>216 bp</td> | ||
</tr> | </tr> | ||
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<td><a href="http://parts.igem.org/Part:BBa_K1727002">BBa_K1727002</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K1727002">BBa_K1727002</a></td> | ||
<td>Coding</td> | <td>Coding</td> | ||
| − | <td><a title="This signal peptide is comes from the signal of chitinase C which extracted by S.lividans. This signal had been proved that can worked in E.coli and successfully secret target protein to E.coli LB culture medium. After translation process signal peptide will lead mature peptide to the secretion system of E.coli. When the pre-mature peptides go through the periplasmic space, peptidase will identified the cleavage site Ala-Gln-Ala and cut at the double Ala at the signal and mature peptide linkage site. Then separate signal peptide from mature peptide. Finally, secreting out mature peptide into the culture medium." href="">Signal peptide</a></td> | + | <td><a title="This signal peptide is comes from the signal of chitinase C which extracted by S.lividans. This signal had been proved that can worked in E.coli and successfully secret target protein to E.coli LB culture medium. After translation process signal peptide will lead mature peptide to the secretion system of E.coli. When the pre-mature peptides go through the periplasmic space, peptidase will identified the cleavage site Ala-Gln-Ala and cut at the double Ala at the signal and mature peptide linkage site. Then separate signal peptide from mature peptide. Finally, secreting out mature peptide into the culture medium." href="">Signal peptide (<I>S.lividans chitinase C</I>)</a></td> |
<td>93 bp</td> | <td>93 bp</td> | ||
</tr> | </tr> | ||
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<td><a href="http://parts.igem.org/Part:BBa_K1727001">BBa_K1727001</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K1727001">BBa_K1727001</a></td> | ||
<td>Coding</td> | <td>Coding</td> | ||
| − | <td><a title="Epinecidin-1 is a kind of antimicrobial peptide (AMPs) and it is a stable peptides that have extensive abilities to kill or inhibit the growth of bacteria. They play a role in defense mechanism for Epinephelus coioides to against microbes. Epinecidin-1 use it's chargeability to interact with bacteria cell membrane. Than use hydrophobic region interfere the membrane structure. This leads to cell lysis and bypasses bacterial antibiotic drug-resistance mechanisms. Moreover Epinecidin-1 has ability to help wound healing and has been proven by animal studies." href="">Epinecidin-1</a></td> | + | <td><a title="Epinecidin-1 is a kind of antimicrobial peptide (AMPs) and it is a stable peptides that have extensive abilities to kill or inhibit the growth of bacteria. They play a role in defense mechanism for Epinephelus coioides to against microbes. Epinecidin-1 use it's chargeability to interact with bacteria cell membrane. Than use hydrophobic region interfere the membrane structure. This leads to cell lysis and bypasses bacterial antibiotic drug-resistance mechanisms. Moreover Epinecidin-1 has ability to help wound healing and has been proven by animal studies." href="">Epinecidin-1 (<I>Epinephelus coioides</I>)</a></td> |
<td>216 bp</td> | <td>216 bp</td> | ||
</tr> | </tr> | ||
| Line 158: | Line 158: | ||
<td><a href="http://parts.igem.org/Part:BBa_K1727002">BBa_K1727002</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K1727002">BBa_K1727002</a></td> | ||
<td>Coding</td> | <td>Coding</td> | ||
| − | <td><a title="This signal peptide is comes from the signal of chitinase C which extracted by S.lividans. This signal had been proved that can worked in E.coli and successfully secret target protein to E.coli LB culture medium. After translation process signal peptide will lead mature peptide to the secretion system of E.coli. When the pre-mature peptides go through the periplasmic space, peptidase will identified the cleavage site Ala-Gln-Ala and cut at the double Ala at the signal and mature peptide linkage site. Then separate signal peptide from mature peptide. Finally, secreting out mature peptide into the culture medium." href="">Signal peptide</a></td> | + | <td><a title="This signal peptide is comes from the signal of chitinase C which extracted by S.lividans. This signal had been proved that can worked in E.coli and successfully secret target protein to E.coli LB culture medium. After translation process signal peptide will lead mature peptide to the secretion system of E.coli. When the pre-mature peptides go through the periplasmic space, peptidase will identified the cleavage site Ala-Gln-Ala and cut at the double Ala at the signal and mature peptide linkage site. Then separate signal peptide from mature peptide. Finally, secreting out mature peptide into the culture medium." href="">Signal peptide (<I>S.lividans chitinase C</I>)</a></td> |
<td>93 bp</td> | <td>93 bp</td> | ||
</tr> | </tr> | ||
Revision as of 15:51, 12 September 2015
This year TCU_Taiwan iGEM team had built new standard biological parts for iGEM community. Click on the name of the parts for detailed information.
Parts table
| Favorite | Name | Type | Description | Legth |
|---|---|---|---|---|
| BBa_K1727000 | Coding | Signiferin with signal peptide | 179 bp | |
| BBa_K1727001 | Coding | Epinecidin-1 (Epinephelus coioides) | 216 bp | |
| BBa_K1727002 | Coding | Signal peptide (S.lividans chitinase C) | 93 bp | |
| BBa_K1727003 | Coding | Epinecidin-1 with signal peptide | 309 bp | |
| BBa_K1727005 | Coding | Signiferin (Crinia signifera) | 275 bp | |
| BBa_K1727007 | Composite | Promoter+Lac operator+RBS+signal peptide +Epinecidin-1+Terminater |
216 bp | |
| BBa_K1727008 | Composite | Promoter+Lac operator+RBS+Signal peptide +Signiferin+Terminater |
179 bp |
Basic parts table
| Favorite | Name | Type | Description | Legth |
|---|---|---|---|---|
| BBa_K1727000 | Coding | Signiferin with signal peptide | 179 bp | |
| BBa_K1727001 | Coding | Epinecidin-1 (Epinephelus coioides) | 216 bp | |
| BBa_K1727002 | Coding | Signal peptide (S.lividans chitinase C) | 93 bp | |
| BBa_K1727003 | Coding | Epinecidin-1 with signal peptide | 309 bp | |
| BBa_K1727005 | Coding | Signiferin (Crinia signifera) | 275 bp |
Signiferin is a kind of antimicrobial peptide (AMPs) that has extensive abilities to kill Methicillin-Resistant Staphylococcus aureus (MRSA). In order to have more efficient to get Signiferin, we treated signal peptide on the upstream of N-terminal to facilitate the peptide production. To make sure the secretion system is work we had modified the amino acid sequence by attached an Ala at the N-terminal of Signiferin and change originally sequence into E.coli codon usage.
Epinecidin-1 is a kind of antimicrobial peptide (AMPs) comes from the skin mucus of Epinephelus coioides. It has extensive abilities to kill or inhibit the growth of bacteria. Moreover Epinecidin-1 has ability to help wound healing and has been proven by animal studies.
This signal peptide is comes from the signal of chitinase C that extracted by S.lividans. It had been proved that can worked in E.coli and successfully secret target protein to LB culture medium. At the secretion system of Ecoli peptidase will identify the cleavage site Ala-Gln-Ala and cut at the double Ala at the signal and mature peptide linkage site. Finally, secreting out mature peptide into the culture medium.
Epinecidin-1 is a kind of antimicrobial peptide (AMPs) that has extensive abilities to kill or inhibit the growth of bacteria. Moreover Epinecidin-1 has ability to help wound healing. In order to have more efficient to get Epinecidin-1, we treated signal peptide on the upstream N-terminal of Epinecidin-1 to facilitate the peptide production. To make sure the secretion system is work we had modified Epinecidin-1 amino acid sequence by attached an Ala at the N-terminal of it.
Signiferin is a kind of antimicrobial peptide (AMPs) that has extensive abilities to kill or inhibit the growth of bacteria. It plays a role in defense mechanism for Crinia signifera to against microbes. Signiferin has demonstrated effectiveness in killing Methicillin-Resistant Staphylococcus aureus (MRSA), and has been proven by the TU-Delft 2013 iGEM team.
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