Difference between revisions of "Team:ATOMS-Turkiye/Extras/Notebook"

Line 238: Line 238:
 
<p>Results weren’t matching with expected results, experiment will be repeated.</p>
 
<p>Results weren’t matching with expected results, experiment will be repeated.</p>
 
<p>GEL GÖRÜNTÜSÜ</p>
 
<p>GEL GÖRÜNTÜSÜ</p>
 +
<br>
  
 +
{| border="1" class="sortable"
 +
!Gradient PCR from pCAGGS (CAG FWD – Chicken β Aktin REVERSE)
 +
|-
 +
|||MgCl₂||(NH₄)2SO₄||VR fwd||VR rv||dNTP||Tag||ddH₂O||DNA||Total
 +
|-
 +
|1x||2.5 ul||2.5 ul||0.5 ul||0.5 ul||0.5 ul||0.2 ul||16.3 ul||2.0 ul||25.0 ul
 +
|-
 +
|9X||22.5 ul||22.5 ul||4.5 ul||4.5 ul||4.5 ul||1.8 ul||146.7 ul||18.0 ul||225.0 ul
 +
|}
 +
<p>60-68˚C</p>
 +
<p>Results weren’t matching with expected results, experiment will be repeated.</p>
 +
<p>GEL GÖRÜNTÜSÜ</p>
 +
<br>
 +
 +
Protocols of „Phusion Pol, and Q5 Polymerase” (02.07.2015)
 +
{| border="1" class="sortable"
 +
!Phusion DNA Polymerase
 +
|-
 +
|||dNTP||Fwd primer||Rev primer||Buffer||Phusion Pol.||ddH₂O||DNA||Total
 +
|-
 +
|1x||0.4 ul||2.0 ul||2.0 ul||4.0 ul||0.2 ul||10.4 ul||1.0 ul||20.0 ul
 +
|-
 +
|
 +
|-
 +
|Cycling
 +
|-
 +
|||98˚C||98˚C||???˚C||72˚C||72˚C||Cycle
 +
|-
 +
|Time||2’||10’’||30’’||1’||5’||???x
 +
|-
 +
|
 +
|-
 +
|
 +
|-
 +
|Q5 DNA Polymerase
 +
|-
 +
|||dNTP||Fwd primer||Rev primer||Buffer||Q5 Pol.||ddH₂O||DNA||Total
 +
|-
 +
|1x||0.5 ul||2.5 ul||2.5 ul||5.0 ul||0.25 ul||8.25 ul||1.0 ul||20.0 ul
 +
|-
 +
|
 +
|-
 +
|Cycling
 +
|-
 +
|||98˚C||98˚C||???˚C||72˚C||72˚C||Cycle
 +
|-
 +
|Time||2’||10’’||30’’||1’||5’||???x
 +
|}
 
   
 
   
  

Revision as of 09:39, 14 September 2015


NOTEBOOK

December

Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description..



January

Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description..



February

Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description..



March

Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description..



April

Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description..



May

Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description..



June

Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description..


Gradient PCR of „pCAG with CMV-Enhancer FWD/Cβ-Actin REV. Primers” (29.06.2015)

Gradient PCR from pCAGGS
MgCl₂ (NH₄)2SO₄ VR fwd VR rv dNTP Tag ddH₂O DNA Total
1x 2.5 ul 2.5 ul 0.5 ul 0.5 ul 0.5 ul 0.2 ul 16.3 ul 2.0 ul 25.0 ul
9X 22.5 ul 22.5 ul 4.5 ul 4.5 ul 4.5 ul 1.8 ul 146.7 ul 18.0 ul 225.0 ul

57-64˚C

Results weren’t matching with expected results, experiment will be repeated.

GEL GÖRÜNTÜSÜ


(30.06.2015)

Gradient PCR from pCAGGS
MgCl₂ (NH₄)2SO₄ VR fwd VR rv dNTP Tag ddH₂O DNA Total
1x 2.5 ul 2.5 ul 0.5 ul 0.5 ul 0.5 ul 0.2 ul 16.3 ul 2.0 ul 25.0 ul
9X 22.5 ul 22.5 ul 4.5 ul 4.5 ul 4.5 ul 1.8 ul 146.7 ul 18.0 ul 225.0 ul

52-59˚C

Results weren’t matching with expected results, experiment will be repeated.

GEL GÖRÜNTÜSÜ


July

Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description..

Gradient PCR of „pCAG with CAG FWD/CAG REV. Primers/pCMV REV.” (01.07.2015)

Gradient PCR from pCAGGS (CAG FWD – CAG REVERSE)
MgCl₂ (NH₄)2SO₄ VR fwd VR rv dNTP Tag ddH₂O DNA Total
1x 2.5 ul 2.5 ul 0.5 ul 0.5 ul 0.5 ul 0.2 ul 16.3 ul 2.0 ul 25.0 ul
9X 22.5 ul 22.5 ul 4.5 ul 4.5 ul 4.5 ul 1.8 ul 146.7 ul 18.0 ul 225.0 ul

60-68˚C

Results weren’t matching with expected results, experiment will be repeated.

GEL GÖRÜNTÜSÜ


Gradient PCR from pCAGGS (CAG FWD – Chicken β Aktin REVERSE)
MgCl₂ (NH₄)2SO₄ VR fwd VR rv dNTP Tag ddH₂O DNA Total
1x 2.5 ul 2.5 ul 0.5 ul 0.5 ul 0.5 ul 0.2 ul 16.3 ul 2.0 ul 25.0 ul
9X 22.5 ul 22.5 ul 4.5 ul 4.5 ul 4.5 ul 1.8 ul 146.7 ul 18.0 ul 225.0 ul

60-68˚C

Results weren’t matching with expected results, experiment will be repeated.

GEL GÖRÜNTÜSÜ


Protocols of „Phusion Pol, and Q5 Polymerase” (02.07.2015)

Phusion DNA Polymerase
dNTP Fwd primer Rev primer Buffer Phusion Pol. ddH₂O DNA Total
1x 0.4 ul 2.0 ul 2.0 ul 4.0 ul 0.2 ul 10.4 ul 1.0 ul 20.0 ul
Cycling
98˚C 98˚C ???˚C 72˚C 72˚C Cycle
Time 2’ 10’’ 30’’ 1’ 5’ ???x
Q5 DNA Polymerase
dNTP Fwd primer Rev primer Buffer Q5 Pol. ddH₂O DNA Total
1x 0.5 ul 2.5 ul 2.5 ul 5.0 ul 0.25 ul 8.25 ul 1.0 ul 20.0 ul
Cycling
98˚C 98˚C ???˚C 72˚C 72˚C Cycle
Time 2’ 10’’ 30’’ 1’ 5’ ???x


Design of acid resistance.. Design of acid resistance.. Design of acid resistance.. Design of acid resistance.. Design of acid resistance.. Design of acid resistance.. Design of acid resistance..

Results of acid resistance.. Results of acid resistance.. Results of acid resistance.. Results of acid resistance.. Results of acid resistance.. Results of acid resistance.. Results of acid resistance..

Results of acid resistance.. Results of acid resistance.. Results of acid resistance.. Results of acid resistance.. Results of acid resistance.. Results of acid resistance.. Results of acid resistance..

August

Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description..

Background of acid resistance.. Background of acid resistance.. Background of acid resistance.. Background of acid resistance.. Background of acid resistance.. Background of acid resistance..

Design of acid resistance.. Design of acid resistance.. Design of acid resistance.. Design of acid resistance.. Design of acid resistance.. Design of acid resistance.. Design of acid resistance..

Results of acid resistance.. Results of acid resistance.. Results of acid resistance.. Results of acid resistance.. Results of acid resistance.. Results of acid resistance.. Results of acid resistance..

Results of acid resistance.. Results of acid resistance.. Results of acid resistance.. Results of acid resistance.. Results of acid resistance.. Results of acid resistance.. Results of acid resistance..

September

Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description.. Some description..

Background of acid resistance.. Background of acid resistance.. Background of acid resistance.. Background of acid resistance.. Background of acid resistance.. Background of acid resistance..

Design of acid resistance.. Design of acid resistance.. Design of acid resistance.. Design of acid resistance.. Design of acid resistance.. Design of acid resistance.. Design of acid resistance..

Results of acid resistance.. Results of acid resistance.. Results of acid resistance.. Results of acid resistance.. Results of acid resistance.. Results of acid resistance.. Results of acid resistance..

Results of acid resistance.. Results of acid resistance.. Results of acid resistance.. Results of acid resistance.. Results of acid resistance.. Results of acid resistance.. Results of acid resistance..