Difference between revisions of "Team:TU Dresden/Notebook/PlasmidDesign"
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<h3>Plasmid Maps</h3> | <h3>Plasmid Maps</h3> | ||
<h4>M13 gene 3 coding for infection protein P3</h4> | <h4>M13 gene 3 coding for infection protein P3</h4> | ||
| + | For more information click <a style="text-decoration:none;" href="https://static.igem.org/mediawiki/2015/b/b3/TU_Dresden_geneIII_first.pdf"><font color="#045FB4">here</font></a> | ||
<figure align="center"> | <figure align="center"> | ||
| − | <a href="https://static.igem.org/mediawiki/2015/ | + | <a href="https://static.igem.org/mediawiki/2015/e/e8/TU_Dresden_geneIII_first_cut.jpg" title="View"> |
<IMG SRC="https://static.igem.org/mediawiki/2015/e/e8/TU_Dresden_geneIII_first_cut.jpg" style="width: 50%;"/></a> | <IMG SRC="https://static.igem.org/mediawiki/2015/e/e8/TU_Dresden_geneIII_first_cut.jpg" style="width: 50%;"/></a> | ||
| − | |||
| − | |||
</figure> | </figure> | ||
<h4>HER2 (Human Epidermal Growth Factor 2) cytoplasmic domain</h4> | <h4>HER2 (Human Epidermal Growth Factor 2) cytoplasmic domain</h4> | ||
| − | + | For more information click <a style="text-decoration:none;" href="https://static.igem.org/mediawiki/2015/3/3a/TU_Dresden_her2_first.pdf"><font color="#045FB4">here</font></a> | |
| − | + | <figure align="center"> | |
| − | + | <a href="https://static.igem.org/mediawiki/2015/3/3a/TU_Dresden_her2_first.pdf" title="View"> | |
| + | <IMG SRC="https://static.igem.org/mediawiki/2015/b/b7/TU_Dresden_her2_first_cut.jpg" style="width: 50%;"/></a> | ||
| + | </figure> | ||
| + | |||
<h4>ZHER2 - affibody with affinity to HER2</h4> | <h4>ZHER2 - affibody with affinity to HER2</h4> | ||
| − | + | For more information click <a style="text-decoration:none;" href="https://static.igem.org/mediawiki/2015/d/d9/TU_Dresden_zher2_first.pdf"><font color="#045FB4">here</font></a> | |
| − | + | <figure align="center"> | |
| − | + | <a href="https://static.igem.org/mediawiki/2015/e/e0/TU_Dresden_zher2_affibody_first_cut.jpg" title="View"> | |
| + | <IMG SRC="https://static.igem.org/mediawiki/2015/e/e0/TU_Dresden_zher2_affibody_first_cut.jpg" style="width: 50%;"/></a> | ||
| + | </figure> | ||
| + | |||
<h4>T25 - subunit of BACTH system</h4> | <h4>T25 - subunit of BACTH system</h4> | ||
| − | + | For more information click <a style="text-decoration:none;" href="https://static.igem.org/mediawiki/2015/2/26/TU_Dresden_t25_first.pdf"><font color="#045FB4">here</font></a> | |
| − | + | <figure align="center"> | |
| − | + | <a href="https://static.igem.org/mediawiki/2015/7/75/TU_Dresden_t25_bacth_first_cut.jpg" title="View"> | |
| + | <IMG SRC="https://static.igem.org/mediawiki/2015/7/75/TU_Dresden_t25_bacth_first_cut.jpg" style="width: 50%;"/></a> | ||
| + | </figure> | ||
| + | |||
<h4>T18 - subunit of BACTH system</h4> | <h4>T18 - subunit of BACTH system</h4> | ||
| − | + | For more information click <a style="text-decoration:none;" href="https://static.igem.org/mediawiki/2015/3/38/TU_Dresden_t18_first.pdf"><font color="#045FB4">here</font></a> | |
| − | + | <figure align="center"> | |
| − | + | <a href="https://static.igem.org/mediawiki/2015/0/0b/TU_Dresden_t18_bacth_first_cut.jpg" title="View"> | |
| + | <IMG SRC="https://static.igem.org/mediawiki/2015/0/0b/TU_Dresden_t18_bacth_first_cut.jpg" style="width: 50%;"/></a> | ||
| + | </figure> | ||
| + | |||
| + | |||
<h4>Leucine zipper able to bind to T25 BACTH subunit</h4> | <h4>Leucine zipper able to bind to T25 BACTH subunit</h4> | ||
| − | + | For more information click <a style="text-decoration:none;" href="https://static.igem.org/mediawiki/2015/5/57/TU_Dresden_lzt25_first.pdf"><font color="#045FB4">here</font></a> | |
| − | + | <figure align="center"> | |
| − | + | <a href="https://static.igem.org/mediawiki/2015/a/a3/TU_Dresden_lzt25_bacth_first_cut.jpg" title="View"> | |
| + | <IMG SRC="https://static.igem.org/mediawiki/2015/a/a3/TU_Dresden_lzt25_bacth_first_cut.jpg" style="width: 50%;"/></a> | ||
| + | </figure> | ||
| + | |||
<h4>Leucine zipper able to bind to T18 BACTH subunit</h4> | <h4>Leucine zipper able to bind to T18 BACTH subunit</h4> | ||
| − | + | For more information click <a style="text-decoration:none;" href="https://static.igem.org/mediawiki/2015/1/1f/TU_Dresden_lzt18_first.pdf"><font color="#045FB4">here</font></a> | |
| − | + | <figure align="center"> | |
| − | + | <a href="https://static.igem.org/mediawiki/2015/c/cd/TU_Dresden_lzt18_bacth_first_cut.jpg" title="View"> | |
| + | <IMG SRC="https://static.igem.org/mediawiki/2015/c/cd/TU_Dresden_lzt18_bacth_first_cut.jpg" style="width: 50%;"/></a> | ||
| + | </figure> | ||
</div> | </div> | ||
</html> | </html> | ||
Revision as of 18:12, 14 September 2015
Plasmid Design
All plasmids designed in this section were used as parts for the various subprojects. These plasmids were initially designed to have kanomycin selection marker. These were synthesized from IDT. Below, the plasmid maps of the various parts characterized as for this project can be found.
Please refer to the Parts section for the final updated parts in iGEM Biobrick format.
Plasmid Maps
M13 gene 3 coding for infection protein P3
For more information click here
HER2 (Human Epidermal Growth Factor 2) cytoplasmic domain
For more information click here
ZHER2 - affibody with affinity to HER2
For more information click here
T25 - subunit of BACTH system
For more information click here
T18 - subunit of BACTH system
For more information click here
Leucine zipper able to bind to T25 BACTH subunit
For more information click here
Leucine zipper able to bind to T18 BACTH subunit
For more information click here