Difference between revisions of "Team:Paris Saclay/Notebook/August/24"
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=Monday 24th August= | =Monday 24th August= | ||
==Lab Work== | ==Lab Work== | ||
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''by Audrey'' | ''by Audrey'' | ||
− | + | * BBa_K1707037 #1 to #10 | |
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===Electrophoresis=== | ===Electrophoresis=== | ||
''by Audrey'' | ''by Audrey'' | ||
− | |||
* BBa_K1707004 #5 (digested by SpeI + EcoRI) | * BBa_K1707004 #5 (digested by SpeI + EcoRI) | ||
* BBa_K1707022 (digested by XbaI + PstI) | * BBa_K1707022 (digested by XbaI + PstI) | ||
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''by Audrey'' | ''by Audrey'' | ||
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* BBa_K1707004 #5 and BBa_K1707023 #1: | * BBa_K1707004 #5 and BBa_K1707023 #1: | ||
** 10 µL plasmid | ** 10 µL plasmid | ||
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** 3 µL Buffer FasrDigest 10x | ** 3 µL Buffer FasrDigest 10x | ||
** 5 µL H2O | ** 5 µL H2O | ||
− | |||
Incubation 1h30, 37°C | Incubation 1h30, 37°C | ||
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'''Member present:''' | '''Member present:''' |
Latest revision as of 07:37, 15 September 2015
Monday 24th August
Lab Work
New culture
by Audrey
- BBa_K1707037 #1 to #10
Electrophoresis
by Audrey
- BBa_K1707004 #5 (digested by SpeI + EcoRI)
- BBa_K1707022 (digested by XbaI + PstI)
Agarose gel 1%, Migration 90V
We don't have any gel picture because the gel broked. We should do this electrophoresis again.
Digestion
by Audrey
- BBa_K1707004 #5 and BBa_K1707023 #1:
- 10 µL plasmid
- 1 µL SpeI
- 1 µL EcoRI
- 2 µL Buffer 2 NEB
- 6 µL H2O
- BBa_K1707004 #5 and BBa_K1707023 #1:
- 10 µL plasmid
- 1 µL SpeI
- 1 µL PstI
- 2 µL Buffer 2 NEB
- 6 µL H2O
- BBa_K1707022 #1:
- 15 µL plasmid
- 1 µL XbaI
- 1 µL PstI
- 2 µL Buffer FastDigest 10x
- 1 µL H2O
- BBa_R0040:
- 20 µL plasmid
- 1 µL XbaI
- 1 µL EcoRI
- 3 µL Buffer FasrDigest 10x
- 5 µL H2O
Incubation 1h30, 37°C
Member present:
- Instructors: Claire
- Students: Audrey