All the work was done by students : wiki design , fundraising , policy and practices as all the cakes shown on social networks. The iGEM Bordeaux team created the Cur’d Vine project thanks to the original idea of Marie CECILLE, member of our team, who proposed to work on Downy Mildew since the beginning of team brainstorming sessions. We then divided the team in groups in order to work at the same time on different topics and read a large panel of publications. Most of figures on the wiki were made by Emilie GOUNIN,member of our team, during incubation times. During the internship, all of the designs, constructs, and experiments were made by us but the synthesis of DNA was made by IDT technologies. Our protocols are based on many publications (all cited in each protocols files) or generously given by our host lab. Between June to August, nine students stayed at lab and worked on two specified studies; one on bacteria and one on yeast. On yeast work, Derek McCUSKER, PhD Group Leader, CNRSIBGC - UMR 5095 (CNRS-UBS) helped us by explaining the homologous repair to express the FKS1 gene and on the design of primers for this gene. Denis DUPUY PhD Group Leader of INSERM/Université Bordeaux Segalen (U869); ARN: Régulations naturelle et artificielle" (ARNA) ; our supervisor ; led us for the Gibson chew back assembly technique and Jonathan MILLET, Denis DUPUY’s PhD student, gave us some advices for PCR troubleshooting. On bacteria work, Camila PARROT member of INSERM / Université Bordeaux Segalen (Unit 869) NA: Natural and Artificial Regulation” (ARNA) led by Martin TEICHMANN, helped to optimize our purification of curdlan. Lionel BEAUREPAIRE, INSERM engineer, gave many advices on clonage. On Plant tests Anthony Bellee member of INRA/ (unit SAVE) Michel Hernould researcher at INRA for all the precious devices. Concerning our wiki, it was based on Evry's 2014 team wiki. We decided to adapt their code for our wiki. We also received a great help of Nacim BENALI, student in informatics, for the design of the wiki. The comics were designed by Caroline GAGNANT, one of our classmates. On Ethics section, M. Jacques FAUCHER’s interview helped us to understand the utility of ethics committees.
During our entire project, many people helped us to accomplish our project Cur’d Vine. First, we want to thank the European Institute of Chemistry and Biology for hosting us all the year and during our internships. Denis DUPUY, our supervisor who gives us many judicious advices and Jonathan MILLET who succeeded to coexist with many team members of iGEM Bordeaux during all this summer. Our team had to use several machines which not belong to our lab. That’s why we had to collaborate many times with the neighbor labs. We hope that they enjoyed our cake as gift. ;) Thanks to: - Aurore DE RACHE, Amina BEDRAT, Nassima GUEDDOUDA and Oscar MENDOZA from Jean-Louis Mergny’s Group to lend us their spectrophometer, their NanoDrop instruments and their Fluorescence Reader. - Martin TEICHMANN's group to lend us incubators and the pHmeter - Natacha PEREBASKINE from Axel INNIS’ group, for the safety formation and for the lending of their Nanodrop instruments - Myriam MEDERIC for her hard work - Camila PARROT for her daily support Moreover, we want to thank Didier THORAVAL, teacher and researcher of "Laboratoire de Biogenèse Membranaire" gave us many resources for our work on yeast. Without this help, yeast work will never start. Also, we are grateful that M. McIntosh helped us by answering to our questions on Curdlan.
At the end, thanks to all other people who supported our project on social media (Facebook, Twitter, Linkedin and Youtube). Your interest had always touched us and we hope that we will support again for 2016 iGEM competition!
Figure 6: strains of agrobacterium producing curdlan (green) observed with a microscope using flurescence (???) SOURCE: Goemar
Register for iGEM, have a great summer, and attend the Giant Jamboree.✔ Complete Judging form.✔ Create a Team Wiki.✔ Present a poster and a talk at the iGEM Jamboree.✔ Create a page on your team wiki with clear attribution of each aspect of your project. ✔ Look at our Attributions Page Document at least one new standard BioBrick Part or Device central to your project and submit this part to the iGEM Registry. You may also document a new application of a BioBrick part from a previous iGEM year, adding that documentation to the part main page.✔
Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected. Document the characterization of this part in the Main Page section of that Part’s/Device’s Registry entry. This working part must be different to the part documented in bronze medal criteria✔ Submit this new part to the iGEM Parts Registry. Your part must be different to the submission for Bronze medal criteria #6.✔ iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, and intellectual property rights. Demonstrate how your team has identified, investigated and addressed one or more of these issues in the context of your project. Your activity could center around education, public engagement, public policy issues, public perception or other activities (See the human practices hub for more information and examples of previous teams exemplary work). ✔
iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, and intellectual property rights. Expand on your silver medal activity by demonstrating how you have integrated the investigated issues into the design and/or execution of your project OR demonstrate an innovative human practices activity that relates to your project (this typically involves educational, public engagement, and/or public perception activities; see the human practices hub for information and examples of previous teams comprehensive and innovative activities).✔ Convince the judges you have helped any registered iGEM team from a high-school, different track, another university, or institution in a significant way by, for example, mentoring a new team, characterizing a part, debugging a construct, modeling/simulating their system or helping validate a software/hardware solution to a synbio problem.✔ Improve the function OR characterization of an previously existing BioBrick Part or Device (created by another team or your own institution in a previous year of the iGEM competition) and enter this information in the Registry. Please see the Registry help page on how to document a contribution to an existing part. This part must not come from your 2015 part range.✔ Demonstrate a functional prototype of your project. Your prototype can derive from a previous project (that was not demonstrated to work) by your team or by another team. Show this system working under real-world conditions (biological materials may not be taken outside the lab).✔