Difference between revisions of "Team:KU Leuven/InterLabStudy/Results"
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The minipreped samples of the given devices were validated by restriction mapping using the enzymes NcoI and XhoI. These restriction enzymes left us with bands around 267, 364, 625 and 1724 basepairs. This was validated by a gel electrophoresis (figure 1). The numbers 101, 106 and 117 stand for the devices containing the promoters J23101, J23106 and J23117 respectively. | The minipreped samples of the given devices were validated by restriction mapping using the enzymes NcoI and XhoI. These restriction enzymes left us with bands around 267, 364, 625 and 1724 basepairs. This was validated by a gel electrophoresis (figure 1). The numbers 101, 106 and 117 stand for the devices containing the promoters J23101, J23106 and J23117 respectively. | ||
</p> | </p> | ||
+ | |||
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+ | <div class="tg-wrap"><table class="tg" style="width:100%;border-collapse:separate"> | ||
+ | <tr> | ||
+ | <th class="tg-title">Protein</th> | ||
+ | <th class="tg-title">K<sub>d</sub> (molecules)</th> | ||
+ | <th class="tg-title">n</th> | ||
+ | <th class="tg-title">Source</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-dark">cI</td> | ||
+ | <td class="tg-dark">20</td> | ||
+ | <td class="tg-dark">2</td> | ||
+ | <td class="tg-dark"> 2009 iGEM Aberdeen and Wang et al. (2009) </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-light">LuxR/AHL</td> | ||
+ | <td class="tg-light">3.7</td> | ||
+ | <td class="tg-light">1</td> | ||
+ | <td class="tg-light"> 2007 iGEM ETH Zurich and Basu et al. (2005) </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-dark">PenI</td> | ||
+ | <td class="tg-dark">17</td> | ||
+ | <td class="tg-dark">2</td> | ||
+ | <td class="tg-dark">Wittman et al. (1993)</td> | ||
+ | </tr> | ||
+ | </table></div> | ||
+ | |||
+ | |||
+ | |||
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Revision as of 14:26, 15 September 2015
Interlab Results
The results of our Interlab study are discussed in this section The minipreped samples of the given devices were validated by restriction mapping using the enzymes NcoI and XhoI. These restriction enzymes left us with bands around 267, 364, 625 and 1724 basepairs. This was validated by a gel electrophoresis (figure 1). The numbers 101, 106 and 117 stand for the devices containing the promoters J23101, J23106 and J23117 respectively.
Protein | Kd (molecules) | n | Source |
---|---|---|---|
cI | 20 | 2 | 2009 iGEM Aberdeen and Wang et al. (2009) |
LuxR/AHL | 3.7 | 1 | 2007 iGEM ETH Zurich and Basu et al. (2005) |
PenI | 17 | 2 | Wittman et al. (1993) |
Figure 1
Signaling cascade for motility in E.coli
Contact
Address: Celestijnenlaan 200G room 00.08 - 3001 Heverlee
Telephone n°: +32(0)16 32 73 19
Mail: igem@chem.kuleuven.be