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Revision as of 14:33, 15 September 2015

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Modeling

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3 models are used to measure our project.

Bacteria scale

We use logistic equation to fit the mono-restrict factor growth of bacteria, and the bacteria scale itself is the factor.

dX/dt=Vmax*(1-X/Xmax)*X

In this equation, ‘X’ means the bacteria scale, it’s the function of‘t’, time. 'Vmax' represents the possible max relative growth speed of the bacteria . 'Xmax' is the upper limit of the growth. In this function, we can find out that the instantaneous growth speed is determined by the relative growth speed, upper limit of the growth, and the instantaneous scale. With time passing by, the scale grows larger, while the speed turns down.

Product scale

We assume that our product comes from two parts, one is during the growth, and the other is through their livelihood.

dP/dt=α*dX/dt+β*X

In this equation, ‘X’ and ‘t’ are the bacteria scale and time. ‘P’ means the product. We can see the familiar ‘X’ and ‘t’, that is how we base the production on the bacteria growth. 'α' and 'β' indicates the proportion of the two processes. With these parameters, we can find out when is the most important part of time in the whole fermentation process. This is quite useful, because we can decide the length of fermentation and decide the timing to do some changes.

Substrate scale

Bacteria use the provided carbon source to propagate, maintain their livelihood and synthesize the product.

dS/dt=-γ*dX/dt-δ*X-ε*dP/dt

In this equation, 'S' is the substrate, 'γ', 'δ',and 'ε' are the ratio of the three outlets. Our genetic operation is aimed to make the bacteria put more energy into the product. In a way, these parameters can show whether our strategies are effective or not.

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