Difference between revisions of "Template:SJTU-BioX-Shanghai/Notebook/Transformation/w7"
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====pre-experiment of pdark-HR==== | ====pre-experiment of pdark-HR==== | ||
| − | + | =====In the evening of Aug. 20===== | |
Inoculated 16 wildtype & 16 pdark-HR (use pdark-HR from electro-transformation), added 11g/L or 22g/L NaCl & retinene at the beginning | Inoculated 16 wildtype & 16 pdark-HR (use pdark-HR from electro-transformation), added 11g/L or 22g/L NaCl & retinene at the beginning | ||
| − | + | =====In the evening of Aug. 22===== | |
Induced with darkness, took the first 4 samples at 9pm | Induced with darkness, took the first 4 samples at 9pm | ||
| − | + | =====In the evening of Aug. 23===== | |
Cultivated with white light, took the second 4 samples at 9pm. | Cultivated with white light, took the second 4 samples at 9pm. | ||
Latest revision as of 08:33, 16 September 2015
Contents
Transformation
pdark natural transformation
In the evening of Aug 18
We picked 4 colonies (marked with 1,2,3 & 4) from the pdark-HR plate and spread them on another BG11 plate in parallel seprately to do solid segregation
In the evening of Aug. 20
We scraped the grown cell from the plate, which is 1,3 & 4 to do colony PCR, 1st liquid segregation and solid storage
pre-experiment of pdark-HR
In the evening of Aug. 20
Inoculated 16 wildtype & 16 pdark-HR (use pdark-HR from electro-transformation), added 11g/L or 22g/L NaCl & retinene at the beginning
In the evening of Aug. 22
Induced with darkness, took the first 4 samples at 9pm
In the evening of Aug. 23
Cultivated with white light, took the second 4 samples at 9pm.