<figcaption><br><b>Figure 1</b> SOE PCR of Prefix-His-Tag-GFP. The size of the amplified product 1 was around 0.9 kbp. DNA marker: Quick-Load Purple 2-Log DNA Ladder.</figcaption>
<figcaption><br><b>Figure 1</b> SOE PCR of Prefix-His-Tag-GFP. The size of the amplified product 1 was around 0.9 kbp. DNA marker: Quick-Load Purple 2-Log DNA Ladder.</figcaption>
<figcaption><br><b>Figure 2</b> SOE PCR of Prefix-His-Tag-GFP-Si4. The size of the amplified product 2 was around 1.1 kbp. DNA marker: Quick-Load Purple 2-Log DNA Ladder.</figcaption>
<figcaption><br><b>Figure 3</b> Colony PCR of B0034-Prefix-His-Tag-GFP-Si4. Colony 1 contained the insert of around 1.1 kbp. DNA marker: Quick-Load Purple 2-Log DNA Ladder.</figcaption>
Bba_K1602025 was used as template for SOE-PCR with oligonucleotides VF2 and 33. The silica-tag Si4 was added using another SOE-PCR with oligonucleotides VF2 and VR. A PCR with oligonucleotides 1 and VR added the RBS to the construct. The PCR product was purified and digested with EcoRI and PstI. The construct was ligated into pSB1C3 and transformed into E. coli Top 10 via heat shock. Colonies were screened by colony PCR with VF2 and VR. The plasmids were extracted and digested with XbaI and PstI and ligated into B0034-pSB1A2 and transformed into E. coli Top 10. Colonies were screened by colony PCR with oligonucleotides VF2 and VR. Positive colonies were inoculated and the plasmids were extracted
Figure 1 SOE PCR of Prefix-His-Tag-GFP. The size of the amplified product 1 was around 0.9 kbp. DNA marker: Quick-Load Purple 2-Log DNA Ladder.
