Difference between revisions of "Team:TU Darmstadt/Notebook/sec1/K1602029"

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<figcaption><br><b>Figure 1</b> Colony PCR of T7-B0034-Prefix-His-Tag-GFP-Si4. All colonies (1-3) contained the insert of around 1.1 kbp. DNA marker: Quick-Load Purple 2-Log DNA Ladder..</figcaption>
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<figcaption><br><b>Figure 1</b> Colony PCR of T7-B0034-Prefix-His-Tag-GFP-Si4. All colonies (1-3) contained the insert of around 1.1 kbp. DNA marker: Quick-Load Purple 2-Log DNA Ladder.</figcaption>
 
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Revision as of 19:16, 16 September 2015

K1602029 - T7-B0034-HistidinTag-Green fluorescent protein-SilicaTag4 (T7-B0034-His-GFP-Si4)


Bba_K1602028 was digested with XbaI and PstI and ligated into T7-pSB1A2. Competent E. coli Top 10 were transformed via heat shock. Colonies were screened using a colony PCR with VF2 and VR.

Figure 1 Colony PCR of T7-B0034-Prefix-His-Tag-GFP-Si4. All colonies (1-3) contained the insert of around 1.1 kbp. DNA marker: Quick-Load Purple 2-Log DNA Ladder.