Difference between revisions of "Team:TU Darmstadt/Notebook/sec1/K1602028"

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<figcaption><br><b>Figure 3</b> Colony PCR of B0034-Prefix-His-Tag-GFP-Si4 (1). Colony 1 contained the insert of around  1.1 kbp. DNA marker: Quick-Load Purple 2-Log DNA Ladder.
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<figcaption><br><b>Figure 1</b> Colony PCR of B0034-Prefix-His-Tag-GFP-Si4 (1). Colony 1 contained the insert of around  1.1 kbp. DNA marker: Quick-Load Purple 2-Log DNA Ladder.
 
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Revision as of 20:56, 16 September 2015

K1602028 - B0034-HistidinTag-Green fluorescent protein-SilicaTag4 (B0034-His-GFP-Si4)


Bba_K1602027 was digested with XbaI and PstI and ligated into B0034-pSB1A2 and transformed into E. coli Top 10. Colonies were screened by colony PCR with oligonucleotides VF2 and VR. Positive colonies were inoculated and the plasmids were extracted

Figure 1 Colony PCR of B0034-Prefix-His-Tag-GFP-Si4 (1). Colony 1 contained the insert of around 1.1 kbp. DNA marker: Quick-Load Purple 2-Log DNA Ladder.