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| − | | + | {{:Team:Brasil-USP/Templates/Foot}} |
| − | </tbody>
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| − | | + | |
| − | <thead><h2 align=center>August</h2>
| + | |
| − | | + | |
| − | </thead>
| + | |
| − | | + | |
| − | </tbody>
| + | |
| − | | + | |
| − | <table style="width:100%">
| + | |
| − | <tr>
| + | |
| − | <th style="background-color:#bfacc0">Monday</th>
| + | |
| − | <th style="background-color:#bfacc0">Tuesday</th>
| + | |
| − | <th style="background-color:#bfacc0">Wednesday</th>
| + | |
| − | <th style="background-color:#bfacc0">Thursday</th>
| + | |
| − | <th style="background-color:#bfacc0">Friday</th>
| + | |
| − | <th style="background-color:#bfacc0">Saturday</th>
| + | |
| − | <th style="background-color:#bfacc0">Sunday</th>
| + | |
| − | </tr> <tr>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td>01/08
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
| + | |
| − | </ul>
| + | |
| − | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li>Glycerol Stock</li>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>02/08</td>
| + | |
| − | <td>03/08</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>04/08
| + | |
| − | <ul>
| + | |
| − | <li>Inoculum</li>
| + | |
| − | <ul>
| + | |
| − | <li>E. coli DH5-alpha</li>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a></li>
| + | |
| − | <li>Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
| + | |
| − | </ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li>
| + | |
| − | </ul>
| + | |
| − | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li>Prepare more inoculum</li>
| + | |
| − | <li>Repeat <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>05//08
| + | |
| − | <ul>
| + | |
| − | <li>Prepare assemblies <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a> for cytometry characterization</li>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li>Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
| + | |
| − | </ul>
| + | |
| − | <li>Inoculum</li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li>
| + | |
| − | </ul>
| + | |
| − | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
| + | |
| − | <li>And once again! <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> (this <a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a> is tough)</li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>06/08</td>
| + | |
| − | <td>07/08
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>08/08</td>
| + | |
| − | <td>09/08</td>
| + | |
| − | <td>10/08</td>
| + | |
| − | </tr> <tr>
| + | |
| − | <td>11/08
| + | |
| − | <ul>
| + | |
| − | <li>Prepare Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> for sequencing</li>
| + | |
| − | <li>Analyse the RBS+qteE and RBS+lasR sequencing file</li>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>12/08
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li>RBS+qteE</li>
| + | |
| − | <li>RBS+lasR</li>
| + | |
| − | <li>OBS: this digestion was performed so we clone this parts in the pSB1C3 vector to put them in Biobrick standard</li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>13/08
| + | |
| − | <ul>
| + | |
| − | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>14/08
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a> and <a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a> in pSB1C3 for BBiobrick standard:</li>
| + | |
| − | <ul>
| + | |
| − | <li>RBS+qteE</li>
| + | |
| − | <li>RBS+lasR</li>
| + | |
| − | </ul>
| + | |
| − | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li>
| + | |
| − | <li>OBS: we had some problems here with the purification and we lost all our digestions, so we're repeating the digestion</li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>15/08</td>
| + | |
| − | <td>16/08
| + | |
| − | <ul>
| + | |
| − | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>17/08
| + | |
| − | <ul>
| + | |
| − | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | </tr> <tr>
| + | |
| − | <td>18/08
| + | |
| − | <ul>
| + | |
| − | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
| + | |
| − | </ul>
| + | |
| − | <li>Prepare LB medium (liquid and solid)</li>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>19/08
| + | |
| − | <ul>
| + | |
| − | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>20/08
| + | |
| − | <ul>
| + | |
| − | <li>RBS+qteE, RBS+lasR and Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li>Inoculum</li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>21/08
| + | |
| − | <ul>
| + | |
| − | <li>RBS+qteE, RBS+lasR and Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li>Glycerol Stock</li>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf">Miniprep</a></li>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>22/08
| + | |
| − | <ul>
| + | |
| − | <li>Prepare LB medium (liquid and solid)</li>
| + | |
| − | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li>Repeat <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">restriction analysis</a> adding the enzyme NdeI.</li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>23/08</td>
| + | |
| − | <td>24/08</td>
| + | |
| − | </tr> <tr>
| + | |
| − | <td>25/08
| + | |
| − | <ul>
| + | |
| − | <li>Inoculum</li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a></li>
| + | |
| − | <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
| + | |
| − | </ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> with higher melting temperature.</li>
| + | |
| − | <ul>
| + | |
| − | </td>
| + | |
| − | <td>26/08
| + | |
| − | <ul>
| + | |
| − | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a></li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>27/08
| + | |
| − | <ul>
| + | |
| − | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a></li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>28/08</td>
| + | |
| − | <td>29/08</td>
| + | |
| − | <td>30/08</td>
| + | |
| − | <td>31/08</td>
| + | |
| − | </tr>
| + | |
| − | </table>
| + | |
| − | <br>
| + | |
| − | <br>
| + | |
| − | <br>
| + | |
| − | | + | |
| − | <thead><h2 align=center>September</h2>
| + | |
| − | | + | |
| − | </thead>
| + | |
| − | | + | |
| − | <tbody>
| + | |
| − | | + | |
| − | <table style="width:100%">
| + | |
| − | <tr>
| + | |
| − | <th style="background-color:#bfacc0">Monday</th>
| + | |
| − | <th style="background-color:#bfacc0">Tuesday</th>
| + | |
| − | <th style="background-color:#bfacc0">Wednesday</th>
| + | |
| − | <th style="background-color:#bfacc0">Thursday</th>
| + | |
| − | <th style="background-color:#bfacc0">Friday</th>
| + | |
| − | <th style="background-color:#bfacc0">Saturday</th>
| + | |
| − | <th style="background-color:#bfacc0">Sunday</th>
| + | |
| − | </tr> <tr>
| + | |
| − | <td>01/09
| + | |
| − | <ul>
| + | |
| − | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a> of the part BII with EcoRV.
| + | |
| − | </ul>
| + | |
| − | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>02/09
| + | |
| − | <ul>
| + | |
| − | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>03/09
| + | |
| − | <ul>
| + | |
| − | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li>Inoculum</li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>04/09
| + | |
| − | <ul>
| + | |
| − | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li>Glycerol Stock</li>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>05/09</td>
| + | |
| − | <td>06/09</td>
| + | |
| − | <td>07/09</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>08/09
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/6/63/QuickChange_PCR.pdf" style="color:#f05151">QuickChange PCR</a> to mutate the cleavage site of the <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037</a> to a Cathepsin S recognition site</li>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the QuickCHange PCR product</li>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>09/09</td>
| + | |
| − | <td>10/09
| + | |
| − | <ul>
| + | |
| − | <li>The QuickChange protocol didn't work well. So try again with more attention!</li>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/6/63/QuickChange_PCR.pdf" style="color:#f05151">QuickChange PCR</a> to mutate the cleavage site of the <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037</a> to a Cathepsin S recognition site</li>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the QuickCHange PCR product</li>
| + | |
| − | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>11/09
| + | |
| − | <ul>
| + | |
| − | <li>Prepare inoculum of the mutated <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037<a/></li>
| + | |
| − | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>12/09
| + | |
| − | <ul>
| + | |
| − | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151">Transformation</a></li>
| + | |
| − | </ul>
| + | |
| − | <li>We had far to many difficulties trying to remove the RBS from the <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a>, so we decided to synthesize it using PCR.</li>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/3/35/PCR_for_synthesis.pdf" style="color:#f05151">PCR for synthesis</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a> (<a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> without RBS)</li>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>13/09
| + | |
| − | <ul>
| + | |
| − | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a>
| + | |
| − | <ul>
| + | |
| − | <li>Inoculum</li>
| + | |
| − | </ul>
| + | |
| − | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li>Repeat <a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
| + | |
| − | </ul>
| + | |
| − | <li>Prepare LB medium (solid and liquid)</li>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>14/09
| + | |
| − | <ul>
| + | |
| − | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a>
| + | |
| − | <ul>
| + | |
| − | <li>Glycerol Stock</li>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
| + | |
| − | </ul>
| + | |
| − | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | </tr> <tr>
| + | |
| − | <td>15/09
| + | |
| − | <ul>
| + | |
| − | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
| + | |
| − | </ul>
| + | |
| − | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li>Inoculum</li>
| + | |
| − | </ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>16/09
| + | |
| − | <ul>
| + | |
| − | <li>Prepare 40% glycerol solution</li>
| + | |
| − | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li>Glycerol Stock</li>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>17/09
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> of the synthesized <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a> in TOPO vector followed by <a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
| + | |
| − | </ul>
| + | |
| − | | + | |
| − | <ul>
| + | |
| − | <li>Measurement Interlab Study - Samples growth biobrick devices 1, 2 and 3</li>
| + | |
| − | </ul>
| + | |
| − |
| + | |
| − | </td>
| + | |
| − | <td>18/09
| + | |
| − | <ul>
| + | |
| − | <li>Replate <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a>. We forgot the X-gal :(</li>
| + | |
| − | </ul>
| + | |
| − | <ul>
| + | |
| − | <li>Measurement Interlab Study - Sample preparation and characterization by Flow Cytometry and Fluorometry</li>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>19/09</td>
| + | |
| − | <td>20/09</td>
| + | |
| − | <td>21/09</td>
| + | |
| − | </tr> <tr>
| + | |
| − | <td>22/09
| + | |
| − | <ul>
| + | |
| − | <li>Inoculum of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li>
| + | |
| − | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − |
| + | |
| − | </td>
| + | |
| − | <td>23/09
| + | |
| − | <ul>
| + | |
| − | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
| + | |
| − | </ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf">Miniprep</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>24/09
| + | |
| − | <ul>
| + | |
| − | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>25/09
| + | |
| − | <ul>
| + | |
| − | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li>Inoculum</li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>26/09
| + | |
| − | <ul>
| + | |
| − | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li>Glycerol Stock</li>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
| + | |
| − | </ul>
| + | |
| − | <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>27/09</td>
| + | |
| − | <td>28/09</td>
| + | |
| − | </tr> <tr>
| + | |
| − | <td>29/09
| + | |
| − | <ul>
| + | |
| − | <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>30/09
| + | |
| − | <ul>
| + | |
| − | <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li>Inoculum</li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | </tr>
| + | |
| − | </table>
| + | |
| − | <br>
| + | |
| − | <br>
| + | |
| − | <br>
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | <thead><h2 align=center>October</h2>
| + | |
| − | | + | |
| − | </thead>
| + | |
| − | | + | |
| − | <tbody>
| + | |
| − | | + | |
| − | <table style="width:100%">
| + | |
| − | <tr>
| + | |
| − | <th style="background-color:#bfacc0">Monday</th>
| + | |
| − | <th style="background-color:#bfacc0">Tuesday</th>
| + | |
| − | <th style="background-color:#bfacc0">Wednesday</th>
| + | |
| − | <th style="background-color:#bfacc0">Thursday</th>
| + | |
| − | <th style="background-color:#bfacc0">Friday</th>
| + | |
| − | <th style="background-color:#bfacc0">Saturday</th>
| + | |
| − | <th style="background-color:#bfacc0">Sunday</th>
| + | |
| − | </tr> <tr>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td>01/10
| + | |
| − | <ul>
| + | |
| − | <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li>Glycerol Stock</li>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>02/10</td>
| + | |
| − | <td>03/10</td>
| + | |
| − | <td>04/10</td>
| + | |
| − | <td>05/10</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>06/10
| + | |
| − | <ul>
| + | |
| − | <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
| + | |
| − | </ul>
| + | |
| − | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
| + | |
| − | <ahref="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>07/10
| + | |
| − | <ul>
| + | |
| − | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>08/10
| + | |
| − | <ul>
| + | |
| − | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
| + | |
| − | </ul>
| + | |
| − | <ul>
| + | |
| − | </td>
| + | |
| − | <td>09/10
| + | |
| − | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
| + | |
| − | <ul>
| + | |
| − | <li>Inoculum</li>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>10/10
| + | |
| − | <ul>
| + | |
| − | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
| + | |
| − | <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
| + | |
| − | </ul>
| + | |
| − | </ul>
| + | |
| − | </td>
| + | |
| − | <td>11/10</td>
| + | |
| − | <td>12/10</td>
| + | |
| − | </tr> <tr>
| + | |
| − | <td>13/10</td>
| + | |
| − | <td>14/10</td>
| + | |
| − | <td>15/10</td>
| + | |
| − | <td>16/10</td>
| + | |
| − | <td>17/10</td>
| + | |
| − | <td>18/10</td>
| + | |
| − | <td>19/10</td>
| + | |
| − | </tr> <tr>
| + | |
| − | <td>20/10</td>
| + | |
| − | <td>21/10</td>
| + | |
| − | <td>22/10</td>
| + | |
| − | <td>23/10</td>
| + | |
| − | <td>24/10</td>
| + | |
| − | <td>25/10</td>
| + | |
| − | <td>26/10</td>
| + | |
| − | </tr> <tr>
| + | |
| − | <td>27/10</td>
| + | |
| − | <td>28/10</td>
| + | |
| − | <td>29/10</td>
| + | |
| − | <td>30/10</td>
| + | |
| − | <td>31/10</td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | </tr>
| + | |
| − | </table>
| + | |
| − | | + | |
| − | </html>
| + | |
