Difference between revisions of "Team:Brasil-USP/Notebook/Calendar"

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<h1 align=center><strong>Life Inside the LAB</strong></h1>
 
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<thead><h2 align=center>June</h2>
 
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</thead>
 
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<tbody>
 
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<table width="100%" border="1">
 
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  <tr>
 
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    <th style="background-color:#bfacc0">Monday</th>
 
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    <th style="background-color:#bfacc0">Tuesday</th>
 
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    <th style="background-color:#bfacc0">Wednesday</th>
 
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    <th style="background-color:#bfacc0">Thursday</th>
 
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    <th style="background-color:#bfacc0">Friday</th>
 
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    <th style="background-color:#bfacc0">Saturday</th>
 
−
    <th style="background-color:#bfacc0">Sunday</th>
 
−
  </tr> <tr>
 
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    <td> 02/06
 
−
    <ul>
 
−
      <li>  iGEM kit arrived! :D </li>
 
−
    </ul>
 
−
    </td>
 
−
    <td>01/07
 
−
    <ul>
 
−
      <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a> of lasR <a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> product </li>
 
−
    </ul>
 
−
    </td>
 
−
    <td>02/07
 
−
    <ul>
 
−
      <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the Biobricks:</li>
 
−
      <ul>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_K143012" style="color:#3ab473">BBa_K143012</a></li>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_K081001" style="color:#3ab473">BBa_K081001</a></li>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_E0840" style="color:#3ab473">BBa_E0840</a></li>
 
−
      </ul>
 
−
      <li>Inoculum of the Biobricks sent by iGEM HQ:</li>
 
−
      <ul>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037<a/></li>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_K316018" style="color:#3ab473">BBa_K316018<a/></li>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_K316015" style="color:#3ab473">BBa_K316015<a/></li>
 
−
      </ul>
 
−
    </ul>
 
−
    </td>
 
−
    <td>03/07
 
−
    <ul>
 
−
      <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the Biobricks sent by the Imperial College Team:</li>
 
−
      <ul>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016<a/></li>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_K143031" style="color:#3ab473">BBa_K143031<a/></li>
 
−
      </ul>
 
−
      <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, Quantification of the plasmidial DNA samples and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li>
 
−
      <ul>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037<a/></li>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_K316018" style="color:#3ab473">BBa_K316018<a/></li>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_K316015" style="color:#3ab473">BBa_K316015<a/></li>
 
−
      </ul>
 
−
    </ul>
 
−
    </td>
 
−
    <td>04/07</td>
 
−
    <td>05/07</td>
 
−
    <td>06/07
 
−
    <ul>
 
−
      <li>Inoculum:</li>
 
−
      <ul>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_K143012" style="color:#3ab473">BBa_K143012</a></li>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_K081001" style="color:#3ab473">BBa_K081001</a></li>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_E0840" style="color:#3ab473">BBa_E0840</a></li>
 
−
      </ul>
 
−
    </ul>
 
−
    </td>
 
−
  </tr>
 
−
<tr>
 
−
    <td>07/07
 
−
    <ul>
 
−
      <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, Quantification and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li>
 
−
      <ul>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_K143012" style="color:#3ab473">BBa_K143012</a></li>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_K081001" style="color:#3ab473">BBa_K081001</a></li>
 
−
        <li<a href="http://parts.igem.org/Part:BBa_E0840" style="color:#3ab473">BBa_E0840</a></li>
 
−
      </ul>
 
−
      <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li>
 
−
      <ul>
 
−
        <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
 
−
      </ul>
 
−
    </ul>
 
−
    </td>
 
−
    <td>08/07</td>
 
−
    <td>09/07
 
−
    <ul>
 
−
    <li>Inoculum:</li>
 
−
      <ul>
 
−
        <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
 
−
      </ul>
 
−
    </ul>
 
−
    </td>
 
−
    <td>10/07
 
−
    <ul>
 
−
      <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, Quantification and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li>
 
−
      <ul>
 
−
        <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a> (restriction analysis fail, repeat)</li>
 
−
      </ul>
 
−
      <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> the qteE gene for amplification. We also added the RBS (<a href="http://parts.igem.org/Part:BBa_K143021" style="color:#3ab473">BBa_K143021,</a>) using the foward primer. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this biobrick will be called <a href="http://parts.igem.org/Part:BBa_K1521000" style="color:#3ab473">BBa_K1521000</a>.</li>
 
−
      <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> of RBS+lasR in PTZ57R/T vector (instaclone kit)</li>
 
−
    </ul>
 
−
    </td>
 
−
    <td>11/07
 
−
    <ul>
 
−
      <li>Prepare 40% glycerol solution</li>
 
−
      <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li>
 
−
      <ul>
 
−
        <li>RBS + lasR (<a href="http://parts.igem.org/Part:BBa_C0079"style="color:#3ab473">BBa_C0079</a>)</li>
 
−
      </ul>
 
−
      <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a>:</li>
 
−
      <ul>
 
−
        <li>RBS + qteE</li>
 
−
      </ul>
 
−
    </ul>
 
−
    </td>
 
−
    <td>12/07</td>
 
−
    <td>13/07</td>
 
−
  </tr> <tr>
 
−
    <td>14/07
 
−
    <ul>
 
−
      <li>Assembly:<a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a></li>
 
−
      <ul>
 
−
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 
−
      </ul>
 
−
      <li>Prepare X-gal</li>
 
−
      <li>Prepare more LB medium (solid + liquid)</li>
 
−
    </ul>
 
−
    </td>
 
−
    <td>15/07
 
−
    <ul>
 
−
      <li>Assembly:<a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a></li>
 
−
      <ul>
 
−
        <li>Quantification of digestion EXSP products</li>
 
−
      </ul>
 
−
      <li>Replate RBS+lasR</li>
 
−
      <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> of <a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> qteE in pGEM T-easy vector</li>
 
−
      <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li>
 
−
      <ul>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> <a href="" style="color:#3ab473"> BBa_J04450</a></a></li>
 
−
      </ul>
 
−
      <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li>
 
−
      <ul>
 
−
        <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
 
−
      </ul>
 
−
    </ul>
 
−
    </td>
 
−
    <td>16/07
 
−
    <ul>
 
−
    <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151">Transformation</a>:</li>
 
−
      <ul>
 
−
      <li><a href="http://parts.igem.org/Part:BBa_K823003" style="color:#3ab473">BBa_K823003</a></li>
 
−
      <li>RBS+qteE</li>
 
−
      </ul>
 
−
    <li>Electrophoresis analysis:</li>
 
−
      <ul>
 
−
      <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
 
−
      </ul>
 
−
    </ul>
 
−
    </td>
 
−
    <td>17/07
 
−
    <ul>
 
−
      <li>Glycerol Stock and <a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:<li>
 
−
      <ul>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a></li>
 
−
        <li>RBS+lasR</li>
 
−
        <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
 
−
      </ul>
 
−
      <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a>:</li>
 
−
      <ul>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (EP)</li>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (XS)</li>
 
−
      </ul>
 
−
      <li>Inoculum:</li>
 
−
      <ul>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_K823003" style="color:#3ab473">BBa_K823003</a></li>
 
−
      </ul>
 
−
      <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
 
−
      <ul>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
 
−
      </ul>
 
−
    </ul>
 
−
    </td>
 
−
    <td>18/07
 
−
    <ul>
 
−
      <li>Glycerol Stock and <a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:</li>
 
−
      <ul>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a></li>
 
−
        <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
 
−
      </ul>
 
−
      <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a>:</li>
 
−
      <ul>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (EP)</li>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (XS)</li>
 
−
      </ul>
 
−
      <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li>
 
−
      <ul>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_K823003" style="color:#3ab473">BBa_K823003</a></a></li>
 
−
      </ul>
 
−
    </ul>
 
−
    </td>
 
−
    <td>19/07</td>
 
−
    <td>20/07</td>
 
−
  </tr> <tr>
 
−
    <td>21/07
 
−
    <ul>
 
−
      <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li>
 
−
      <ul>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li>
 
−
      </ul>
 
−
      <li>Inoculum:</li>
 
−
      <ul>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
 
−
        <li>RBS+qteE</li>
 
−
      </ul>
 
−
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li>
 
−
      <ul>
 
−
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 
−
      </ul>
 
−
    </ul>
 
−
    </td>
 
−
    <td>22/07
 
−
    <ul>
 
−
      <li>Inoculum:</li>
 
−
      <ul>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li>
 
−
      </ul>
 
−
      <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a>:</li>
 
−
      <ul>
 
−
        <li>RBS+qteE</li>
 
−
      </ul>
 
−
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li>
 
−
      <ul>
 
−
        <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
 
−
        <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
 
−
      </ul>
 
−
    </ul>
 
−
    </td>
 
−
    <td>23/07
 
−
    <ul>
 
−
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li>
 
−
      <ul>
 
−
        <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
 
−
      </ul>
 
−
      <li>Prepare more <a href="https://static.igem.org/mediawiki/2014/9/96/Competent_Cells_using_Calcium_Chloride.pdf" style="color:#f05151"> competent cells</a></li>
 
−
      <li>Prepare LB medium (solid and liquid)</li>
 
−
      <li>Inoculum</li>
 
−
      <ul>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li>
 
−
      </ul>
 
−
      <li>Prepare RBS+lasR and RBS+qteE for sequencing</li>
 
−
    </ul>
 
−
    </td>
 
−
    <td>24/07
 
−
    <ul>
 
−
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li>
 
−
      <ul>
 
−
        <li>Inoculum</li>
 
−
      </ul>
 
−
      <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:</li>
 
−
      <ul>
 
−
        <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li>
 
−
      </ul>
 
−
    </ul>
 
−
    </td>
 
−
    <td>25/07
 
−
    <ul>
 
−
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li>
 
−
      <ul>
 
−
        <li>Glycerol Stock</li>
 
−
        <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
 
−
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
 
−
      </ul>
 
−
    </ul>
 
−
    </td>
 
−
    <td>26/07</td>
 
−
    <td>27/07</td>
 
−
  </tr> <tr>
 
−
    <td>28/07
 
−
    <ul>
 
−
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a>:</li>
 
−
      <ul>
 
−
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> (+NdeI enzyme)</li>
 
−
        <li>OBS: This analysis was reapeted because we had problems confirming the assembly. The issue was that both the construction and the pSB1C3 vector had similar size.</li>
 
−
      </ul>
 
−
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>,
 
−
          <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a> and
 
−
          <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li>
 
−
      <ul>
 
−
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 
−
      </ul>
 
−
    </ul>
 
−
    </td>
 
−
    <td>29/07
 
−
    <ul>
 
−
      <li>>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>,
 
−
          <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a> and
 
−
          <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li>
 
−
      <ul>
 
−
        <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
 
−
        <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
 
−
      </ul>
 
−
    </ul>
 
−
    </td>
 
−
    <td>30/07
 
−
    <ul>
 
−
      <li>>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>,
 
−
          <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a> and
 
−
          <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li>
 
−
      <ul>
 
−
        <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
 
−
      </ul>
 
−
    </ul>   
 
−
    </td>
 
−
    <td>31/07
 
−
    <ul>
 
−
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>,
 
−
          <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a> and
 
−
          <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li>
 
−
      <ul>
 
−
        <li>Inoculum</li>
 
−
      </ul>
 
−
      <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> for RBS removal</li>
 
−
      <li>OBS: This was one of the parts sent by the Imperial College Team. Because of a comunication failure we assumed that this part was comming without the RBS, so we included a RBS in the parts we were going to use with this  Lac promotor :) </li>
 
−
      <li>PCR of Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> was followed by a <a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a> and <a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> in the PUC19 vector</li>
 
−
    </ul>
 
−
    </td>
 
−
    <td></td>
 
−
    <td></td>
 
−
    <td></td>
 
−
  </tr>
 
−
</table>
 
  
 
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