Difference between revisions of "Team:Gifu/Experiment-page"
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<img src="https://static.igem.org/mediawiki/2015/3/3c/Gifu-kinou2.png"width="346" height="137" align="right" vspace="25" hspace="80"></img><br> | <img src="https://static.igem.org/mediawiki/2015/3/3c/Gifu-kinou2.png"width="346" height="137" align="right" vspace="25" hspace="80"></img><br> | ||
The requirements for amino acids that can be good linkers are less of steric effects and strong in hydropathy. Glycine and serine fulfill this requirements. Glycine’ side chain has almost no steric effects. On the other hand, serine has some steric effects but it has powerful hydropathy because of its hydroxyl side chain. According to Mr. Yabe, because histidine has quite strong hydropathy, though it has big steric effects, it is possible that histidine can work as a linker.<br></p> | The requirements for amino acids that can be good linkers are less of steric effects and strong in hydropathy. Glycine and serine fulfill this requirements. Glycine’ side chain has almost no steric effects. On the other hand, serine has some steric effects but it has powerful hydropathy because of its hydroxyl side chain. According to Mr. Yabe, because histidine has quite strong hydropathy, though it has big steric effects, it is possible that histidine can work as a linker.<br></p> | ||
− | <img src="https://static.igem.org/mediawiki/2015/b/b0/Kinouichiran.png"width="400" height="157" align="right" vspace="25" hspace="50"></img | + | <img src="https://static.igem.org/mediawiki/2015/b/b0/Kinouichiran.png"width="400" height="157" align="right" vspace="25" hspace="50"></img> |
Then, we designed some linkers [ see right ]. "GGSGGS", "GSGSGS", and "HHHHHH" were designed as linkers. We combined these sequence and inserted. The pattern of combination was shown right. | Then, we designed some linkers [ see right ]. "GGSGGS", "GSGSGS", and "HHHHHH" were designed as linkers. We combined these sequence and inserted. The pattern of combination was shown right. | ||
− | <br clear="right"><img src="https://static.igem.org/mediawiki/2015/e/e0/Gifu-kinou3.png"width="432" height="224" align="right" vspace="25" hspace="50"></img | + | <br clear="right"><img src="https://static.igem.org/mediawiki/2015/e/e0/Gifu-kinou3.png"width="432" height="224" align="right" vspace="25" hspace="50"></img></br> |
<p> | <p> | ||
Amino acid chains synthesized from circular mRNA are constructed from repetition in amino acid of target protein, amino acid from ribozyme and amino acid from RBS. In this experiment, we inserted linker sequences into inside of both splicing sites. Therefore, protein chains synthesized from circular messenger RNA that include linker sequences repeat linker sequence, amino acid from RBS, target protein, linker sequence and amino acid from ribozyme.<br> | Amino acid chains synthesized from circular mRNA are constructed from repetition in amino acid of target protein, amino acid from ribozyme and amino acid from RBS. In this experiment, we inserted linker sequences into inside of both splicing sites. Therefore, protein chains synthesized from circular messenger RNA that include linker sequences repeat linker sequence, amino acid from RBS, target protein, linker sequence and amino acid from ribozyme.<br> |
Revision as of 04:04, 17 September 2015