Revision as of 11:19, 30 June 2015

iGEM Kent 2015

@@ Line 20: / Line 20: @@
 <a name="Transformation Protocol"></a><h5> Transformation Protocol </h5>
 <a href="#Overview">Overview</a> <br>
-<p>    <li> The Miniprep is for purification of molecular biology grade plasmid DNA </li>
-       <li> This provides a rapid method to purify plasmid DNA using silica membrane column  </li>
-</p>
 <a href="#Materials">Materials</a> <br>
 <a href="#Procedure">Procedure</a> <br>
@@ Line 29: / Line 26: @@
 <a name="Miniprep"></a><h5> Miniprep </h5>
 <a href="#Overview">Overview</a> <br>
+<p>    <li> The Miniprep is for purification of molecular biology grade plasmid DNA </li>
+       <li> This provides a rapid method to purify plasmid DNA using silica membrane column  </li>
+</p>
 <a href="#Materials">Materials</a> <br>
 <a href="#Procedure">Procedure</a> <br>
+<p>    <li> Add the provided RNase A solution to Buffer P1.</li>
+       <li> Mix the solution and store at 2–8°C </li>
+       <li> Add ethanol (96–100%) to Buffer PE before use </li>
+       # Pellet 1–5 ml bacterial overnight culture by centrifugation at >8000 rpm (6800 x g) for 3
+min at room temperature (15–25°C).
+       <li> </li>
+       <li> </li>
+       <li> </li>
+</p>
 <a href="#References">References</a> <br>

Difference between revisions of "Team:Kent/Experiments"