Difference between revisions of "Template:Team:Groningen/CONTENT/EXPERIMENTS/Digestion BBa K823023 and BBa K823024 (t20)"

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<div class="text">The samples were digested for 2 hours at 37 °C. Afterwards they were loaded on 1% agarose gel with SERVA DNA stain G.</div>
 
<div class="text">The samples were digested for 2 hours at 37 °C. Afterwards they were loaded on 1% agarose gel with SERVA DNA stain G.</div>
  
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<div class="object data" id="tbl1">
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<div class="wrapper">
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<div class="header">
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<div class="field fw1">#</div>
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<div class="field fw3">Component</div>
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<div class="field fw7">Amount</div>
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</div>
 +
<div class="record">
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<div class="field fw1">1</div>
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<div class="field fw3">DNA (tasA 4.2)</div>
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<div class="field fw7">20 µL</div>
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</div>
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<div class="record">
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<div class="field fw1"></div>
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<div class="field fw3">6x buffer</div>
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<div class="field fw7">4 µL</div>
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</div>
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<div class="record">
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<div class="field fw1">2</div>
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<div class="field fw3">DNA (BBa_K823023)</div>
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<div class="field fw7">25 µL</div>
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</div>
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<div class="record">
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<div class="field fw1"></div>
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<div class="field fw3">6x buffer</div>
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<div class="field fw7">5 µL</div>
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</div>
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<div class="record">
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<div class="field fw1">3</div>
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<div class="field fw3">DNA (BBa_K823024)</div>
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<div class="field fw7">25 µL</div>
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</div>
 +
<div class="record">
 +
<div class="field fw1"></div>
 +
<div class="field fw3">6x buffer</div>
 +
<div class="field fw7">5 µL</div>
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</div>
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</div>
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<div class="caption">Samples for on the gel.</div>
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</div>
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<div class="text">The gel was run on 100 V for ∼1 hour and the following bands were visible: </div>
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<div class="listing">
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<div class="item">tasA at ∼800-900 bp</div>
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<div class="item">K823023 (∼5000 bp) including a band of its RFP insert (∼1100 bp)</div>
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<div class="item">K823024 (∼6000 bp) including a band of its RFP insert (∼1100 bp)</div>
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</div>
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<div class="text">These band sizes match the in silico sizes.
 
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Revision as of 02:10, 18 September 2015

00:00, 2 June 2015 - 00:00, 2 June 2015
The backbones BBa_K823023 and BBa_K823024 were digested and ligated with tasA by the following steps.
#
Component
Amount
1
Mastermix
28 µL
DNA (BBa_K823023)
2 µL
2
Mastermix
28 µL
DNA (BBa_K823024)
2 µL
Digestion mixtures.
The samples were digested for 2 hours at 37 °C. Afterwards they were loaded on 1% agarose gel with SERVA DNA stain G.


#
Component
Amount
1
DNA (tasA 4.2)
20 µL
6x buffer
4 µL
2
DNA (BBa_K823023)
25 µL
6x buffer
5 µL
3
DNA (BBa_K823024)
25 µL
6x buffer
5 µL
Samples for on the gel.
The gel was run on 100 V for ∼1 hour and the following bands were visible:
tasA at ∼800-900 bp
K823023 (∼5000 bp) including a band of its RFP insert (∼1100 bp)
K823024 (∼6000 bp) including a band of its RFP insert (∼1100 bp)
These band sizes match the in silico sizes.
Harm Ruesink