Difference between revisions of "Template:Team:Groningen/CONTENT/EXPERIMENTS/Digestion BBa K823023 and BBa K823024 (t20)"
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<div class="text">The samples were digested for 2 hours at 37 °C. Afterwards they were loaded on 1% agarose gel with SERVA DNA stain G.</div> | <div class="text">The samples were digested for 2 hours at 37 °C. Afterwards they were loaded on 1% agarose gel with SERVA DNA stain G.</div> | ||
+ | |||
+ | |||
+ | <div class="object data" id="tbl1"> | ||
+ | <div class="wrapper"> | ||
+ | <div class="header"> | ||
+ | <div class="field fw1">#</div> | ||
+ | <div class="field fw3">Component</div> | ||
+ | <div class="field fw7">Amount</div> | ||
+ | </div> | ||
+ | <div class="record"> | ||
+ | <div class="field fw1">1</div> | ||
+ | <div class="field fw3">DNA (tasA 4.2)</div> | ||
+ | <div class="field fw7">20 µL</div> | ||
+ | </div> | ||
+ | <div class="record"> | ||
+ | <div class="field fw1"></div> | ||
+ | <div class="field fw3">6x buffer</div> | ||
+ | <div class="field fw7">4 µL</div> | ||
+ | </div> | ||
+ | <div class="record"> | ||
+ | <div class="field fw1">2</div> | ||
+ | <div class="field fw3">DNA (BBa_K823023)</div> | ||
+ | <div class="field fw7">25 µL</div> | ||
+ | </div> | ||
+ | <div class="record"> | ||
+ | <div class="field fw1"></div> | ||
+ | <div class="field fw3">6x buffer</div> | ||
+ | <div class="field fw7">5 µL</div> | ||
+ | </div> | ||
+ | <div class="record"> | ||
+ | <div class="field fw1">3</div> | ||
+ | <div class="field fw3">DNA (BBa_K823024)</div> | ||
+ | <div class="field fw7">25 µL</div> | ||
+ | </div> | ||
+ | <div class="record"> | ||
+ | <div class="field fw1"></div> | ||
+ | <div class="field fw3">6x buffer</div> | ||
+ | <div class="field fw7">5 µL</div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="caption">Samples for on the gel.</div> | ||
+ | </div> | ||
+ | |||
+ | <div class="text">The gel was run on 100 V for ∼1 hour and the following bands were visible: </div> | ||
+ | |||
+ | <div class="listing"> | ||
+ | <div class="item">tasA at ∼800-900 bp</div> | ||
+ | <div class="item">K823023 (∼5000 bp) including a band of its RFP insert (∼1100 bp)</div> | ||
+ | <div class="item">K823024 (∼6000 bp) including a band of its RFP insert (∼1100 bp)</div> | ||
+ | </div> | ||
+ | |||
+ | <div class="text">These band sizes match the in silico sizes. | ||
}} | }} |
Revision as of 02:10, 18 September 2015
00:00, 2 June 2015 - 00:00, 2 June 2015
The backbones BBa_K823023 and BBa_K823024 were digested and ligated with tasA by the following steps.
#
Component
Amount
1
Mastermix
28 µL
DNA (BBa_K823023)
2 µL
2
Mastermix
28 µL
DNA (BBa_K823024)
2 µL
The samples were digested for 2 hours at 37 °C. Afterwards they were loaded on 1% agarose gel with SERVA DNA stain G.
#
Component
Amount
1
DNA (tasA 4.2)
20 µL
6x buffer
4 µL
2
DNA (BBa_K823023)
25 µL
6x buffer
5 µL
3
DNA (BBa_K823024)
25 µL
6x buffer
5 µL
The gel was run on 100 V for ∼1 hour and the following bands were visible:
tasA at ∼800-900 bp
K823023 (∼5000 bp) including a band of its RFP insert (∼1100 bp)
K823024 (∼6000 bp) including a band of its RFP insert (∼1100 bp)
These band sizes match the in silico sizes.