Difference between revisions of "Template:Team:Groningen/CONTENT/LOGBOOK/tasA Ligation t22"

Latest revision as of 02:37, 18 September 2015

tasA K823023 Ligation (t22)

In this experiment tasA was ligated with the K823023 backbone to make transformation possible.

Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.

The K823023 backbone was ligated with tasA.

<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/Ligation">Ligation</a>

00:00, 3 June 2015 - 00:00, 3 June 2015

For the ligation of tasA in BBa_K823023, the following components were used.

Vector DNA (K823023)

2 µL

Insert DNA (tasA)

2 µL

10x buffer

2 µL

T4 ligase

1 µL

\( \mathrm{H_2O}\)

13 µL

Components used for ligation.

The ligation mixture was left 2 hours at room temperature.

Harm Ruesink

@@ Line 4: / Line 4: @@
 	|protocolurl=Ligation
 	|goal=<html>Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.</html>
-	|description=In this experiment tasA was ligated with the K823023 backbone to make transformation in Bacillus possible.
+	|description=In this experiment tasA was ligated with the K823023 backbone to make transformation possible.
 	|conclusion=The K823023 backbone was ligated with tasA.
 }}