Difference between revisions of "Team:Nankai/Basic Part"
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<p style="position: relative; top: 0px; left: 20px; width: 700px; font-size: 18px; font-family: calibri,Arial, Helvetica, sans-serif; text-align: justify; line-height: 30px;"> According to our promoter strength assay (showed in Figure 1), <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628001">BBa_K1628001</a> (P<sub>bca</sub>), <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628003">BBa_K1628003</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (BJ27UP<sub></sub>), <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628004">BBa_K1628004</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (C2up<sub></sub>), <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628005">BBa_K1628005</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (A2up<sub></sub>), <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628006">BBa_K1628006</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (P43), and <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628007">BBa_K1628007</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (P<sub>amyA</sub>) show different properties. Promoter P<sub>bca</sub> is the original promoter of <em>pgsBCA</em> operon.Promoter BJ27UP C2up and A2up are an artificially synthesized promoters. Promoter P43 is a strong promoter in <em>Bacillus subtilis</em> 168. Promoter P<sub>amyA</sub> is a strong promoter in <em>Bacillus amyloliquefaciens</em> LL3.</p> | <p style="position: relative; top: 0px; left: 20px; width: 700px; font-size: 18px; font-family: calibri,Arial, Helvetica, sans-serif; text-align: justify; line-height: 30px;"> According to our promoter strength assay (showed in Figure 1), <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628001">BBa_K1628001</a> (P<sub>bca</sub>), <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628003">BBa_K1628003</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (BJ27UP<sub></sub>), <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628004">BBa_K1628004</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (C2up<sub></sub>), <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628005">BBa_K1628005</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (A2up<sub></sub>), <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628006">BBa_K1628006</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (P43), and <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628007">BBa_K1628007</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (P<sub>amyA</sub>) show different properties. Promoter P<sub>bca</sub> is the original promoter of <em>pgsBCA</em> operon.Promoter BJ27UP C2up and A2up are an artificially synthesized promoters. Promoter P43 is a strong promoter in <em>Bacillus subtilis</em> 168. Promoter P<sub>amyA</sub> is a strong promoter in <em>Bacillus amyloliquefaciens</em> LL3.</p> | ||
<p style="position: relative; top: 0px; left: 20px; width: 700px; font-size: 18px; font-family: calibri,Arial, Helvetica, sans-serif; text-align: justify; line-height: 30px;">Judging from the promoter strength assay, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628004">BBa_K1628004</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (C2up<sub></sub>)<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> is the strongest promoters we used in our project and <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628007">BBa_K1628007</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (P<sub>amyA</sub>) is the second strongest promoter in our project. The strength of <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628001">BBa_K1628001</a> (P<sub>bca</sub>) is very weak. While the strength of <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628003">BBa_K1628003</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (BJ27UP<sub></sub>) is stronger than P<sub>bca</sub>, it is still too weak compared with other promoters. What's more, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628005">BBa_K1628005</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (A2up<sub></sub>) is a very strong promoter and <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628006">BBa_K1628006</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (P43) is a weak promoter.</p> | <p style="position: relative; top: 0px; left: 20px; width: 700px; font-size: 18px; font-family: calibri,Arial, Helvetica, sans-serif; text-align: justify; line-height: 30px;">Judging from the promoter strength assay, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628004">BBa_K1628004</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (C2up<sub></sub>)<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> is the strongest promoters we used in our project and <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628007">BBa_K1628007</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (P<sub>amyA</sub>) is the second strongest promoter in our project. The strength of <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628001">BBa_K1628001</a> (P<sub>bca</sub>) is very weak. While the strength of <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628003">BBa_K1628003</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (BJ27UP<sub></sub>) is stronger than P<sub>bca</sub>, it is still too weak compared with other promoters. What's more, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628005">BBa_K1628005</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (A2up<sub></sub>) is a very strong promoter and <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628006">BBa_K1628006</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (P43) is a weak promoter.</p> | ||
| − | <img src="https://static.igem.org/mediawiki/2015/c/c6/Nankai_parts_figure1_blue.jpeg | + | <img src="https://static.igem.org/mediawiki/2015/c/c6/Nankai_parts_figure1_blue.jpeg"> |
| − | < | + | <h6> Figure 1. Promoter strength assay in <em>Bacillus amyloliquefaciens</em> NK-1.</h6> |
<p style="position: relative; top: 0px; left: 20px; width: 700px; font-size: 18px; font-family: calibri,Arial, Helvetica, sans-serif; text-align: justify; line-height: 30px; font-weight: bold;">Coding genes</p> | <p style="position: relative; top: 0px; left: 20px; width: 700px; font-size: 18px; font-family: calibri,Arial, Helvetica, sans-serif; text-align: justify; line-height: 30px; font-weight: bold;">Coding genes</p> | ||
<p style="position: relative; top: 0px; left: 20px; width: 700px; font-size: 18px; font-family: calibri,Arial, Helvetica, sans-serif; text-align: justify; line-height: 30px;"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628101">BBa_K1628101</a> (<em>pgsB</em>) and <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628102">BBa_K1628102</a> (<em>pgsAC</em>) are genes in <em>pgsBCA</em> operon. <em>pgsB</em> is a gene responsible for γ-PGA synthesis.Protein PgsBCA is a membrane protein and subunit PgsB’s main function is gathering substrate glutamic acid for γ-PGA synthesis (showed in Figure 3). Subunit <em>PgsC</em> is responsible for glutamic acid’s polymerization and subunit <em>PgsA</em> is responsible for the secretion of γ-PGA.</p> | <p style="position: relative; top: 0px; left: 20px; width: 700px; font-size: 18px; font-family: calibri,Arial, Helvetica, sans-serif; text-align: justify; line-height: 30px;"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628101">BBa_K1628101</a> (<em>pgsB</em>) and <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628102">BBa_K1628102</a> (<em>pgsAC</em>) are genes in <em>pgsBCA</em> operon. <em>pgsB</em> is a gene responsible for γ-PGA synthesis.Protein PgsBCA is a membrane protein and subunit PgsB’s main function is gathering substrate glutamic acid for γ-PGA synthesis (showed in Figure 3). Subunit <em>PgsC</em> is responsible for glutamic acid’s polymerization and subunit <em>PgsA</em> is responsible for the secretion of γ-PGA.</p> | ||
| − | + | <img src="https://static.igem.org/mediawiki/2015/2/25/Parts_figure3.jpeg"> | |
| − | + | <h6>Figure 3. The synthetic pathway of γ-PGA</h6> | |
<p> </p> | <p> </p> | ||
<p> </p> | <p> </p> | ||
Revision as of 05:15, 18 September 2015
Basic Parts
Promoters
According to our promoter strength assay (showed in Figure 1), BBa_K1628001 (Pbca), BBa_K1628003 (BJ27UP), BBa_K1628004 (C2up), BBa_K1628005 (A2up), BBa_K1628006 (P43), and BBa_K1628007 (PamyA) show different properties. Promoter Pbca is the original promoter of pgsBCA operon.Promoter BJ27UP C2up and A2up are an artificially synthesized promoters. Promoter P43 is a strong promoter in Bacillus subtilis 168. Promoter PamyA is a strong promoter in Bacillus amyloliquefaciens LL3.
Judging from the promoter strength assay, BBa_K1628004 (C2up) is the strongest promoters we used in our project and BBa_K1628007 (PamyA) is the second strongest promoter in our project. The strength of BBa_K1628001 (Pbca) is very weak. While the strength of BBa_K1628003 (BJ27UP) is stronger than Pbca, it is still too weak compared with other promoters. What's more, BBa_K1628005 (A2up) is a very strong promoter and BBa_K1628006 (P43) is a weak promoter.
Figure 1. Promoter strength assay in Bacillus amyloliquefaciens NK-1.
Coding genes
BBa_K1628101 (pgsB) and BBa_K1628102 (pgsAC) are genes in pgsBCA operon. pgsB is a gene responsible for γ-PGA synthesis.Protein PgsBCA is a membrane protein and subunit PgsB’s main function is gathering substrate glutamic acid for γ-PGA synthesis (showed in Figure 3). Subunit PgsC is responsible for glutamic acid’s polymerization and subunit PgsA is responsible for the secretion of γ-PGA.
Figure 3. The synthetic pathway of γ-PGA
