Difference between revisions of "Team:Paris Saclay/Notebook/July/24"

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Agarose gel 1%, 110V
 
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<html><p><i>Verification of purification, from left to right: 1. DNA Ladder, 2. BBa_K1707003#4, 3. BBa_K1399005</i></p></html>
 
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<html><p><i>Quantification, from left to right: 1. DNA Ladder, 2. BBa_K1707003#4, 3. BBa_K1399005, 4. BBa_B0015, 5. BBa_J23101, 6. Empty, 7. Empty</i></p></html>
 
===Transformation===
 
===Transformation===
 
''by Seong-Koo''
 
''by Seong-Koo''

Revision as of 10:03, 18 September 2015


Friday 24th July

Lab Work

Digestion

by Pauline

Biobricks:

  • BBa_K1707003 #4
  • BBa_K1399005
  • BBa_B0015
  • BBa_J23101

In each tube:

  • 10 µL plasmid
  • 1µL of each enzyme
  • 2 µL Buffer FastDigest
  • 6µL H2O

Digestions:

  • BBa_K1707003: XbaI + PstI
  • BBa_K1399005: EcoRI + SpeI
  • BBa_B0015: EcoRI + XbaI
  • BBa_J23101: SpeI + PstI

Purification

by Pauline

Biobricks:

  • BBa_K1707003
  • BBa_K1399005

Agarose gel 1%, 110V

ParisSaclay 24.07.15-vérif purif.jpg

Verification of purification, from left to right: 1. DNA Ladder, 2. BBa_K1707003#4, 3. BBa_K1399005

We cut bands with a scalpel

Purification

by Audrey

Biobricks:

  • BBa_K1707003
  • BBa_K1399005
  • BBa_B0015
  • BBa_J23101

With PCR Clean-up/Gel extraction kit from Macherey-Nigel

Quantification

Biobricks:

  • BBa_K1707003
  • BBa_K1399005
  • BBa_B0015
  • BBa_J23101

Agarose gel 1% Migration 110V

ParisSaclay 24.07.2015 Quantification.jpg

Quantification, from left to right: 1. DNA Ladder, 2. BBa_K1707003#4, 3. BBa_K1399005, 4. BBa_B0015, 5. BBa_J23101, 6. Empty, 7. Empty

Transformation

by Seong-Koo

Biobricks:

  • BBa_K1707005
  • BBa_K1707006
  • BBa_K1707007

Soil experiment

by Seong Koo

Plates observation and count of CFU:

  • Strain 1696:
    • Dilution 1: 424 CFU
    • Dilution 0.1: 75 CFU
    • Dilution 0.01: 1 CFU
  • Strain 1693:
    • Dilution 1: 524 CFU
    • Dilution 0.1: 35 CFU
    • Dilution 0.01: 0 CFU
  • Strain 1320:
    • Dilution 1: 111 CFU
    • Dilution 0.1: 13 CFU
    • Dilution 0.01: 0 CFU

We take 1g of contaminated soil and treat it like yesterday to spread it on specific plates Incubation ON, 37°C

Interlab study: Cytometer

by Johan

https://2015.igem.org/Team:Paris_Saclay/Measurement#24th_July

Member present:

  • Instructors: Alice
  • Students: Coralie, Audrey, Pauline, Johan and Seong-Koo

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