1. What is your chassis organism?

Check all species you are genetically modifying in your project.

• E. coli
• Yeast (Saccharomyces)
• Lactobacillus
• B. subtilis
• Others:
• No chassis organism

Comments:

2. Do you plan to experiment with any other organisms, besides your chassis?

What organisms, and what experiments will you do? Please explain briefly. Please include the names of species / cell lines / strains.

Example answers:

• "Our bacteria is meant to live on plant leaves, so we will test them on tobacco (Nicotiana benthamiana) in a lab greenhouse."
• "We want to use a protein from ants, but its sequence is unknown. So we will capture ants (Camponotus spp.) to extract DNA and RNA to find the sequence of the protein we want."
• "Our bacteria need to interact with human cells for a medical application. We will test them in human cell culture using the HEK293 cell line."

3. How will your project work?

Describe the goal of your project: what is your engineered organism supposed to do? Please include specific technical details and names of important parts. (Even though your project might change, please describe the main project idea you are working on right now. See the example answers for help.)

Good example answers:

• "Our bacteria will live inside a human body. They will detect tumor cells that express biomarkers for liver cancer. They will use invasin to enter the tumor cells, and then secrete apoptin to kill the tumor cells."
• "Our algae will receive the exhaust from a factory, which is high in CO2. We will increase their expression of Photosystem II proteins to make them absorb more CO2, reducing the factory's emissions."

Bad example answers (not enough detail):

• "We are engineering E. coli to cure liver cancer."
• "Climate change is a very important problem. Our algae will reduce CO2 emissions and fight climate change."

4. How would your project be used in the real world?

Imagine that your project were fully developed into a real product that real people could use. How would people use it? Check all appropriate boxes.

(Note: iGEM teams should not release modified organisms into the natural environment.)

• Our project is foundational / we do not have a specific real-world application in mind
  (Examples: library of standardized promoters, system for communication between cells)
• Only in the lab
  (Examples: reporter strain for measuring the strength of promoters)
• In a factory
  (Examples: cells that make a flavor chemical for food, cells that make biofuel)
• In a consumer product that ordinary people buy
  (Examples: cells that clean your clothes, bread made with engineered yeast)
• In agriculture / on a farm
  (Examples: cells that guard against pests, engineered rice plants, cells that promote growth of crop plants)
• In a small enclosed device
  (Examples: a bio-sensing strip with cells that detect arsenic)
• In the natural environment
  (Examples: cells that remove pollution from lakes, engineered forest trees that can resist drought)
• To be used in the human body, or in food
  (Examples: anti-cancer bacteria, bread made with engineered yeast, engineered rice plants)
• Other
  (Examples: bacteria that live on Mars)