Difference between revisions of "NJU-China-notebook.html"
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Here, C57BL/6J mice (n=6 for each group) were purchased from the Model Animal Research Centre (MARC) of Nanjing University (Nanjing, China). The animals received humane care according to the guidelines prepared by the National Institutes of Health Guide for the Care and Use of Laboratory Animals. A two-chamber CPP apparatus (Yishu Software Technology Co. Ltd., Shanghai, China) was used in this study. The two chambers were identical in size but differed in color and floor texture. The two distinct chambers were linked by a smaller intermediate compartment with a shutter on each side that allowed for control of access to either side of the chamber. One chamber had white walls with a barred floor and was illuminated, while the other had black walls with a dotted floor and was not illuminated. </br> | Here, C57BL/6J mice (n=6 for each group) were purchased from the Model Animal Research Centre (MARC) of Nanjing University (Nanjing, China). The animals received humane care according to the guidelines prepared by the National Institutes of Health Guide for the Care and Use of Laboratory Animals. A two-chamber CPP apparatus (Yishu Software Technology Co. Ltd., Shanghai, China) was used in this study. The two chambers were identical in size but differed in color and floor texture. The two distinct chambers were linked by a smaller intermediate compartment with a shutter on each side that allowed for control of access to either side of the chamber. One chamber had white walls with a barred floor and was illuminated, while the other had black walls with a dotted floor and was not illuminated. </br> | ||
Pre-conditioning (Day 1): Mice were allowed free access to both chambers for 30 min, and the number of crossings and the time spent in each chamber were recorded. As recommended by the manufacturer's instructions, animals with fewer than 20 crossings or less than 300 s spent in one chamber were excluded. </br> | Pre-conditioning (Day 1): Mice were allowed free access to both chambers for 30 min, and the number of crossings and the time spent in each chamber were recorded. As recommended by the manufacturer's instructions, animals with fewer than 20 crossings or less than 300 s spent in one chamber were excluded. </br> | ||
− | Conditioning (Days 2–11): On days 2, 4, 6, 8 and 10, mice received a morphine injection (10 mg/kg) before being placed in the white chamber for 30 min. On days 3, 5, 7, 9 and 11, mice received a saline injection (10 ml/kg) before being placed in the black chamber for 30 min. On day 12, all mice were allowed free access to both chambers for 30 min for the CPP test. | + | Conditioning (Days 2–11): On days 2, 4, 6, 8 and 10, mice received a morphine injection (10 mg/kg) before being placed in the white chamber for 30 min. On days 3, 5, 7, 9 and 11, mice received a saline injection (10 ml/kg) before being placed in the black chamber for 30 min. On day 12, all mice were allowed free access to both chambers for 30 min for the CPP test. </br> |
− | Extinction (Days 13-25): All mice were kept in their cages without any treatment. | + | Extinction (Days 13-25): All mice were kept in their cages without any treatment.</br> |
− | Exosome injection (Days 26-32): Before exosome injection, all mice underwent a CPP test. On days 26, 28, 30 and 32, mice were injected intravenously with saline, 200 µg exosomes loaded with MOR siRNA, or 200 µg RVG exosomes loaded with MOR siRNA, respectively. | + | Exosome injection (Days 26-32): Before exosome injection, all mice underwent a CPP test. On days 26, 28, 30 and 32, mice were injected intravenously with saline, 200 µg exosomes loaded with MOR siRNA, or 200 µg RVG exosomes loaded with MOR siRNA, respectively.</br> |
Reinstatement (Days 33-34): On day 33, before being placed in the white chamber for 30 min, all mice received morphine (10 mg/kg). On day 34, all mice underwent a 30 min CPP test. </br> | Reinstatement (Days 33-34): On day 33, before being placed in the white chamber for 30 min, all mice received morphine (10 mg/kg). On day 34, all mice underwent a 30 min CPP test. </br> | ||
<img src="https://static.igem.org/mediawiki/2015/d/d9/NJU-China_Notebook_method5.jpg" style="width:900px"> | <img src="https://static.igem.org/mediawiki/2015/d/d9/NJU-China_Notebook_method5.jpg" style="width:900px"> |
Revision as of 11:32, 18 September 2015
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Notebook
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